37results about How to "Particle rules" patented technology

The invention provides EV71virus-like particles as well as preparation method and application of the EV71virus-like particles. The method comprises the following steps of: connecting a P1 protein gene and 3CD protease gene of an EV71 virus with predicated mean vote (PMV) plasmid to build PMV-P1-3CD recombinant expression plasmid; turning into hansenula polymorpha AU-0501 expression bacterial strain to obtain an AU-PMV-P1-3CD recombinant expression bacterial strain; fermenting and culturing the recombinant expression bacteria strain and performing inducible expression on EV71 virus-like particle proteins by using methanol; centrifugally gathering thallus to perform homogenate crushing at high pressure; and purifying the supernate through ion-exchange column chromatography, hydrophobic chromatography and sieve chromatography to obtain the EV71virus-like particles. EV71virus-like particle vaccines provided by the invention has high immunogenicity, security, immunological characteristics and biological activity; and the EV71virus-like particles can be prepared in a large scale and purified and used for preparing vaccines for preventing EV71 infection, and has good economic value and application prospect.

The invention provides hansenula polymorpha engineering bacteria for efficiently expressing CA10 virus-like particles and application thereof. The engineering bacteria comprise a recombinant vector carrying CA10 virus P1 and 3CD genes, and an original strain of the engineering bacteria is uracil-deficient hansenula polymorpha AU 0501; and the P1 and 3CD genes are optimized according to hansenula polymorpha preferred codons. The invention further provides a preparation method of the CA10 virus-like particles. The preparation method comprises the steps that the engineering bacteria are cultured,the CA10 virus-like particles are expressed, and the virus-like particles are separated and purified by adopting ultrafiltration and three-step chromatography processes. The CA10 virus-like particlesand a vaccine prepared from the CA10 virus-like particles have good immunogenicity, safety and biological activity, the process is simple, purification is conducted by adopting a chromatography method, large-scale preparation and purification can be achieved, a high-purity (greater than 99%) VLP protein stock solution is obtained, and the engineering bacteria can be used for preparing the vaccinefor preventing CA10 infection and have good economic value and application prospects.

The invention discloses a method for preparing high purity nano cobalt hydroxide, belonging to the technical field of battery energy material preparation. Homogeneous phase precipitation method is adopted to synthesize cobalt hydroxide, including that: cobaltous sulphate aqueous solution, sodium hydroxide aqueous solution, cobalt ion chelating agent aqueous solution and aqueous solution of reagent A which are in certain concentration are respectively, sequentially and continuously added into a reactor to be stirred according to flow ratio of 1: 0.68-0.74: 0.048-0.054: 0.058-0.064, reaction is taken place, appropriate amount of additive aqueous solution is added, and ageing, solid-liquid separation, washing and drying are carried out, thus obtaining the high purity nano cobalt hydroxide product. The product not only has high purity, regular shape, small grain size and uniform distribution, but also has high specific area, higher bulk density and good liquidity, dispersity and workability, thus being beneficial to separate processing or uniformly coating cobalt hydroxide on the surface of nickel hydroxide particle by adopting chemical plating, so that combination property of nickel-hydrogen battery is improved.

The invention discloses a preparation method of tri-phosphor double-peak fluorescent powders used for an energy-saving lamp, which has high heat stability and high brightness. The preparation method of the tri-phosphor double-peak blue fluorescent powders for the lamp comprises the steps of firstly weighing each chemical component according to a chemical expression of fluorescent material, then adding BaF2 being 0.1wt%-10.0wt% of the total weight of all chemical components, and CaF2 being 0.01wt%-4.0wt% of the total weight of the all chemical components, sufficiently and uniformly mixing the raw materials, and then burning and reacting sufficiently at 1350-1500 DEG C under the atmosphere of reduction gas and inert gas to obtain a final burning product, then grinding, washing, filtering, drying and screening the burned product to obtain the tri-phosphor double-peak blue fluorescent powders for the lamp. Due to the adoption of the method, the blue fluorescent powders with regular particle morphology, uniform particle size, relatively high brightness and high heat stability, and according to the preparation method, the preparation process is simple, a one-step reduction method is adopted, and the industrial large-scale production is facilitated.

The invention discloses a novel micron-scale up-conversion luminescent material, which is a compound with the following chemical formula: NaYF 4 : Yb, Ln, has the advantages of regular particle shape, uniform particle size, and good dispersibility; the present invention also discloses a novel preparation method for preparing micron-scale up-conversion luminescent materials, using impinging flow-precipitation method, the process involved The process is simple, the energy consumption is small, and the product yield is high, the particle size is small and uniformly distributed, and the luminous intensity is good, which can effectively solve the problems of low yield, complex process flow and poor dispersion of up-conversion luminescent materials in the prior art. The method solves the problems that current up-conversion luminescent materials are difficult to mass-produce, and is suitable for popularization and application.

The invention discloses a fine developer for large-format development and a preparation method thereof, which comprises 75-95% by mass of a bisphenol A crystalline polyester resin binder, 2-15% of an inorganic dye, 1-5 % of olefin oligomer, 0.5-5% of charge regulator, 0.2-5% of charge stabilizer, etc. are fully mixed and then added to a twin-screw extruder for high-temperature extrusion dispersion. The extrusion dispersion temperature is 100-150°C. Fine developer particles of 6-8 μm are obtained by pulverization and classification, and then 0.5-5% developer fluidization agent is mixed and distributed on the surface of the fine developer particles to finally produce large-format fine developer materials. The fine developing material particles obtained by the above method are regular, fine and uniform up to 6-8 μm, have high charge and stability at 20-30 μc / g, and good fluidity above 70%.

The present invention provides a human papilloma virus 16 type L1 protein HPV16L1-g and a coding gene and application thereof. The amino acid sequence of the HPV16L1-g protein is shown as SEQ ID No. 2, and the coding gene is shown as SEQ ID No. 1 in a sequence table. The present invention also provides an HPV16L1-g recombinant expression vector. The HPV16L1-g protein provided by the present invention has good immunogenicity, no potential carcinogenic risk, has good safety, immunological characteristics and biological activity, can be prepared and purified in a large scale, can be used for preparing a vaccine for preventing cervical cancer and drugs for treating cervical cancer, and has good economic values and application prospects.

The invention relates to a preparation method of a lithium iron phosphate / lithium vanadium phosphate composite material. The method comprises the following steps: mixing iron phosphate and vanadium pentoxide which are utilized as raw materials with a lithium salt compound; adding a chelating agent; grinding and mixing; performing heat treatment in an inert atmosphere, and then adding a carbon source material; and heating, roasting and cooling to room temperature to obtain the carbon-coated lithium iron phosphate / lithium vanadium phosphate composite material. The lithium iron phosphate / lithiumvanadium phosphate composite material can be assembled into a testing battery of which the discharge specific capacity can reach 144mAh / g with the current of 150mA / g at room temperature and can reach105mAh / g with the current of 30mA / g at the temperature of minus 20 DEG C, the crystal form is complete, the particle appearance is regular, and excellent normal-temperature and low-temperature electrochemical performances are shown. All the raw materials in the method are bulk industrial chemicals, the cost is low, the process route is simple, the industrial scale production is easy to realize and the application prospect is very broad.

The present invention provides a human papilloma virus 16 type L1 protein HPV16L1-h and a coding gene and application thereof. The amino acid sequence of the HPV16L1-h protein is shown as SEQ ID No. 2, and the coding gene is shown as SEQ ID No. 1 in a sequence table. The present invention also provides an HPV16L1-h recombinant expression vector. The HPV16L1-h protein provided by the present invention has good immunogenicity, no potential carcinogenic risk, has good safety, immunological characteristics and biological activity, can be prepared and purified in a large scale, can be used for preparing a vaccine for preventing cervical cancer and drugs for treating cervical cancer, and has good economic values and application prospects.