43results about How to "Culture conditions can be controlled" patented technology

The invention discloses an induced culture method of tender shoots of aronia melanocarpa. The method comprises steps as follows: a to-be-cultured aronia melanocarpa plant is selected, healthy and strong twigs free from disease and pest damage are sheared in the growing period and subjected to asepsis treatment, an induced culture medium is inoculated with the twigs, induced culture is performed under the proper light and temperature conditions, sterile sprouts in vitro are formed through callus induction and induction of clustered shoots, a primary culture system under the sterile condition isestablished, and a base material is provided for next subculture. The materials are simple and easy to obtain, each link is easy to operate, the culture conditions are controllable, the repeatabilityof result is high, and the method is low in cost, favorable for popularization and input production and has great practical significance for promoting rapid propagation of good seedlings of aronia melanocarpa.

The invention discloses saccharomyces cerevisiae and application thereof in the fermentative production of S-adenosylmethionine. The strain of saccharomyces cerevisiae is classified and named as (saccharomyces cerevisiae) ty-93620 and is preserved in China Microbial Culture Collection Administration General Microbiology Center, with the preservation number of CGMCC No. 13760. The invention also discloses a method for producing S-adenosylmethionine by utilizing the saccharomyces cerevisiae strain capable of achieving high production of S-adenosylmethionine. Compared with a chemical method, the method disclosed by the invention has the advantages of being low in production cost, low in energy consumption, free of pollution, free of crude raw materials, such as non-fossil resources, as raw materials, free of ATP as a catalyst inducer, high in yield, strong in stability, clear and simple in process and easy to operate. Compared with original bacteria, the strain in the invention has fewer types of fermentation products, high purity, good stability and obviously increased yield.

The invention provides a method for producing influenza viruses in large scale by using a bioreactor, which comprises the following steps of: (1) inoculating passage cell suspension into a vector tank, and setting the parameters of the bioreactor to perform a cell adsorption and culture process, wherein a serum culture medium is used in the cell adsorption and culture process; (2) when cells are grown to proper density, rinsing the cells to remove serum components, inoculating influenza virus liquid into the vector tank, and setting the parameters of the bioreactor to perform a virus adsorption and propagation process, wherein a serum-free culture medium containing TPCK (tosyl-L-phenylalanine chloromethyl ketone) pancreatin is used in the virus adsorption and propagation process; and (3) harvesting the virus liquid. The method has the advantage that: the high-titer virus liquid can be obtained by adopting the tide type bioreactor and using the passage cells for propagating the influenza viruses. The method overcomes the defects of the traditional transfer bottle culture and other bioreactors, and can continuously produce high-titer viruses in large scale.

The invention discloses a method for increasing the adhesion rate of juvenile mytilus coruscus. The method for increasing the adhesion rate of the juvenile mytilus coruscus mainly comprises the following steps of (1) obtaining algae species; (2) carrying out primary culture; (3) treating a substratum with benthic diatom; (4) carrying out large-scale culture; and (5) carrying out seedling throwing and seedling raising. The method for increasing the adhesion rate of the juvenile mytilus coruscus is simple in step and convenient to operate, culture conditions are easy to control, after proper algae species (algae species) are selected for culture, a polyethylene film is treated, a layer of firm microbial film capable of inducing attachment of mytilus coruscus planktonic larvae is formed on the surface of the polyethylene film, and the seedling culture conditions are strictly controlled, so that the adhesion rate of the juvenile mytilus coruscus is effectively increased, and a theoretical and practical basis is provided for seedling culture of the mytilus coruscus.

The invention discloses a culture medium and a culture method for increasing the yield of pleurotus eryngii. The culture medium is prepared from the following raw materials in parts by mass: 3 parts of soybean meal, 0.1 part of yeast extract, 3 parts of glucose, 0.05 part of magnesium sulfate, 0.01 part of ferric sulfate, 0.05 part of monopotassium phosphate, 0.001 part of vitamin B1 and 100 parts of water. The method comprises the following steps of pleurotus eryngii hyphae are subjected to passage activation in a solid plate, and hypha blocks with consistent growth conditions are dug from the solid plate by using an inoculation shovel and inoculated into the culture medium; pH, temperature, liquid loading amount and rotating speed are set, and shaking culture is performed on a shaking table for 7 days; all fermentation liquor is filtered by using a 40-mesh screen, supernate is filtered out, vacuum suction filtration is carried out by using weighed qualitative filter paper, the supernate is filtered out, mycelium is washed with water for 3 times, drained, collected, dried in an air dry oven and weighed to constant weight, and the operation is repeated for 3 times; by adopting the fermentation conditions, the strain grows quickly under the process, and the quality of the pleurotus eryngii can be improved.

The invention discloses a method for producing catalpol by using suspension cultured rehmannia stem cambium stem cells, which specifically comprises: using rehmannia plant stems as explants, establishing stable growth of rehmannia root through induction culture, separation culture and subculture layer stem cell culture system; then add combined inducer β-CD and methyl jasmonate in the bioreactor for suspension culture, and then obtain high-purity catalpa after purification by ultrasonic extraction, macroporous resin adsorption, and high-performance liquid phase alcohol. The plant stem cell culture system utilized in the present invention has excellent characteristics such as synchronous growth, stable genetic traits, fast growth rate, and stable synthesis of catalpol, and it provides a new method for the preparation of catalpol as a raw material for preparing catalpol; in addition, Compared with chemical synthesis, the present invention uses bioreactor culture technology combined with plant stem cell culture technology to produce high-purity catalpol, which has the advantages of short production cycle, high safety, low cost, high yield, and low pollution.

The invention relates to an inducing technology of a test tube lotus root, which comprises the steps of: using terminal buds or lateral buds of the lotus root as explants; carrying out the starting of stem tips, successive proliferation and rooting; finally culturing and inducing by using a solid-liquid culture base to form the test tube lotus root. The method for inducing the test tube lotus root comprises the following steps of: cutting the stem tips of the lotus root and inoculating the stem tips on a stem tip starting culture base for culturing so as to grow up to become buds or a cluster of buds; transferring the buds or the cluster of buds to a successive culture base and carrying out successive proliferation; segmenting the cluster of buds into single bud and transferring to a rooting culture base and inducing to root to form tube seedlings; and rooting and culturing for 30d, pouring the liquid culture base into a culture container and inducing to form the test tube lotus root. In the process, the culture temperature is between 24 and 26 DEG C, the illumination intensity is 2000-25001x and the illumination is carried out for 10h every day. Through the stem tip culture and quick reproduction of the lotus root, the test tube lotus root is formed by inducing so as to quicken the reproduction speed of the lotus root, improve the transplant survival rate and industrially produce.

The invention discloses a method for producing soybean isoflavone aglycone through microbe's enzyme method, and is culturing Aspergillus oryzae with soybean isoflavone powder as raw materials for fermentation to produce beta-glucosidase, and converting soybean isoflavone to soybean isoflavone aglycone. The method is characterized in comprising the following preparation steps: the said raw materials are 2-90% soybean isoflavone powder, the said strain is Aspergillus oryzae 3042, preparing seed culture liquid with the strain, preparing solid fermentation medium, fermenting to obtain fermenting product containing beta-glucosidase, adding soybean isoflavone powder for preparing enzyme converting solution, centrifuging for separation to obtain deposit, drying at low temperature to obtain soybean isoflavone aglycone. The invention avoids the restriction of the raw materials purity, converting rata can reach to over 90%. The invention has the advantages of short procedure, low cost, high bioavailability.