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Probiotic feed additive for realizing zero discharge of manure waste and preparation method thereof

A feed additive and probiotic technology, applied in the field of probiotic feed additive and its preparation, can solve the problems of not reaching the intestinal tract, limited application range, affecting the proliferation of bacterial species, etc., so as to increase digestion and utilization rate, and solve the discharge of feces. Pollution and the effect of improving feed utilization

Inactive Publication Date: 2019-03-29
共 放鸣
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The number of viable bacteria is the key to the function of microbial preparations. In actual industrial production, the number of viable bacteria is low, the function of the bacteria is single, and the stability is poor and strongly restricts the scale and industrialization of probiotic products.
[0004] The existing microbial additives have the following defects: 1) The number of viable bacteria is small, especially the poor environmental resistance, such as poor gastric acid resistance, so that they cannot reach the intestinal tract and fail to achieve the effect
2) Long cultivation cycle and long production cycle
3) The effective shelf life of the product is short
4) The microbial additives made of existing compound strains have complex components, and the requirements for the living environment of various beneficial microorganisms are not the same. If it is difficult to control the cultivation conditions during production and use, the quality is difficult to guarantee
5) The scope of application of existing microbial feed additives is limited, and there is no case of combining with other technical fields
The production process is complex, with many intermediate steps, high operation requirements and long production cycle
Difficult management, difficult to achieve large-scale production, high operating costs
Moreover, the direct mixing of multiple strains causes inhibition between the strains and affects the proliferation of the strains
In addition, the solid auxiliary material has a single component and only plays the role of diluting the bacterial powder

Method used

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  • Probiotic feed additive for realizing zero discharge of manure waste and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] refer to figure 1 , a kind of preparation method of the probiotics feed additive that is used for realizing zero-discharge farming of excrement, concrete steps are as follows:

[0041] S1 Add 3~5g of medical stone to every 100g of water, heat and keep the temperature above 60°C for 10-15 minutes, after cooling to a suitable temperature, filter and recover medical stone, and the filtered water is used as water for culture medium.

[0042] S1-1 Preparation of Candida utilis culture medium: Cut 100g of potatoes into small pieces, add 400g of water soaked in medical stone, maintain the temperature at 78-82°C for 60 minutes, filter and replenish the water loss to the total mass 500g to obtain potato dipping juice and store for future use. Take 1000ml of potato juice and add 20.0g of glucose; autoclave at 121°C for 20min.

[0043] S1-2 Preparation of bacterial culture solution of Bacillus licheniformis and Bacillus lentus: add 2.0g of glucose, 1.5g of peptone, 0.5g of sodiu...

Embodiment 2

[0056] refer to figure 1 , a kind of preparation method of the probiotics feed additive that is used for realizing zero-discharge farming of excrement, concrete steps are as follows:

[0057] S1-1 Candida utilis culture medium: cut 100g of potatoes into small pieces, add 400g of water, keep the temperature at 78-82°C for 60 minutes, filter and make up the water loss until the total mass is 500g, and obtain potato juice , stored for later use. Add 2.0 g of glucose to every 100 ml of potato juice; autoclave at 121° C. for 20 min.

[0058] S1-2 Culture solution of Bacillus licheniformis and Bacillus lentus: add 2.2g glucose, 1.7g peptone, 0.6g sodium chloride, 0.06g beef extract per 100ml of water, and autoclave at 121°C for 20min.

[0059] S1-3 Culture solution of Lactobacillus delbrueckii, Lactobacillus plantarum and Lactobacillus reuteri: Add 1.2g peptone, 1.2g beef extract, 0.7g yeast extract, 0.22g diamine hydrogen citrate, 0.22 g glucose, 1.2ml Tween 80, 0.7g sodium acet...

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Abstract

The invention discloses a probiotic feed additive for realizing zero discharge of manure waste. The feed additive for realizing zero discharge of manure waste is characterized by comprising Candida utilis, Bacillus lentus, Bacillus licheniformis, Lactobacillus delbrueckii, Lactobacillus plantarum, Lactobacillus reuteri, and Aspergillus oryzae. The bacterial strain matching used by the probiotic feed additive of the invention has the advantages of improving animal immunity and reducing the risk of disease caused by livestock, does not use antibiotics, and is healthy and environmentally friendly.

Description

technical field [0001] The invention relates to a feed additive and a preparation method thereof, in particular to a probiotic feed additive for realizing zero-discharge farming of manure and a preparation method thereof. Background technique [0002] Animal probiotic feed additives are a type of product that directly feeds animals and regulates the intestinal microecological balance of animals to improve animal health, disease resistance, and digestion. It is an effective means to solve the epidemic of diseases, drug resistance of bacteria, decreased immunity, dependence on antibiotics, decreased survival rate, and decreased breeding benefits. The probiotic effect of probiotics is achieved through a series of reproduction and metabolism of beneficial microorganisms in animals, and its final effect is closely related to the amount of added probiotics. According to the conclusion of the experiment, if the concentration of a probiotic in the intestinal contents is lower than ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K50/30C12N1/20C12N1/14C12N1/16C12R1/72C12R1/07C12R1/10C12R1/225C12R1/25C12R1/69
CPCA23K10/18A23K50/30C12N1/14C12N1/16C12N1/20A23V2400/137A23V2400/173A23V2400/169Y02A40/70
Inventor 共放鸣钟国盛
Owner 共 放鸣
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