The invention discloses a method for sterile seeding and rapid propagation of
bougainvillea speetabilis. The method comprises the steps of conducting sterile seeding and induction
germination on seeds, wherein the seeds are sterilized, then a
shoot induction medium is inoculated with the sterilized seeds, and
shoot induction culture is conducted at proper temperature with proper illumination time and illumination intensity; conducting
seedling breeding through
bud expanding propagation and rooting reinduction, wherein a sprout
enrichment culture medium is inoculated with germinated buds, and
bud propagation subculture is conducted at proper temperature with proper illumination time and illumination intensity; inoculating a rooting medium with the
bud propagation subcultured bud seedlings, and conducting rooting breeding of seedlings at proper temperature with proper illumination time and illumination intensity. According to the method, seeds are used as breeding explant,
seed treatment is easy,
pollution rate is low, the
germination rate of seeds reaches 100%, bud
propagation rate reaches 5.22 times within 30 days, rooting rate is 95.6%, and
seedling breeding is not limited by time or season so that whole-year production can be conducted.