Mesenchymal stem cell and biomaterial combined microsphere and intelligent spraying system

Abstract

The invention discloses a mesenchymal stem cell and biomaterial combined microsphere and an intelligent spraying system. Firstly, sodium alginate is selected as a carrier biomaterial and subjected topartial oxidation treatment, protein components with free amino groups are immobilized through hydrazone bonds, and lithium hydroxide and salicylaldehyde are added into an immobilization process to effectively improve protein component immobilization efficiency of the material; then the hydrazone bonds are reduced by sodium borohydride to obtain C-N single bonds; finally, the protein component immobilized material and stem cells are mixed to prepare cell 3D culture microspheres, the survival rate is effectively increased after stem cell transplantation, and skin wound curative effects of stemcells are improved. Due to the fact that cell products are extremely prone to damages and loss in a transplantation process due to vulnerability, a spray dosing system matched with the microsphere isfurther provided and capable of accurately and efficiently spraying the microsphere to wounded parts, and unnecessary loss and waste are avoided.

Description

technical field [0001] The present invention relates to the biomedical industry, specifically for the collaborative innovation of medical device technology and medical biotechnology, and for the treatment of large-area skin defects, which is a frequently-occurring disease that seriously affects the quality of life, and provides a mesenchymal stem cell combined biological Material microspheres and intelligent spraying system. Background technique [0002] Wound healing is a complex dynamic process that includes distinct and overlapping stages of inflammation, hyperplasia, and remodeling. The harm of a large area of ​​skin defect: first, the primary disease or the large defect area will make the wound difficult to heal and cause complications such as secondary infection; second, chronic inflammation and other factors will lead to epithelial to mesenchymal transition transition) (Li et aL., 2016), localized fibrosis and scarring occur, affecting the appearance and normal funct...

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Problems solved by technology

[0004] However, the general problem of cell replacement therapy is that the cell differentiation efficiency and survival rate after transplantation are low. The main influencing factors are: 1) the change from 2D culture conditions in vitro to 3D tissue microenvironment and tissue composition in vivo; 2) the body's immunity to the graft Rejection and inflammatory reactions; 3) Lack of interaction between cells and cells or extracellular matrix during two-dimensional culture in vitro; 4) Enzymatic digestion or mechanical damage during cell harvesting, etc.

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