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Inducing technology of test tube lotus root

A technology of test-tube lotus root and disease-free lotus root, which is applied in the field of agricultural biology, can solve the problems such as the difficulty in breeding lotus root seeds, and achieve the effects of long-distance transportation, high quality, and high reproduction coefficient

Active Publication Date: 2012-05-23
WUHAN VEGETABLE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention overcomes the technical problem of difficulty in the reproduction of lotus root seeds in the conventional production of lotus root, and provides a test-tube lotus root induction method on the basis of in-depth research on the shoot tip culture and virus-free rapid propagation of lotus root, which is a tissue culture technique for lotus root Applied to production practice provides a more effective method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] In the present invention, the terminal bud of the lotus lotus variety Elian No. 5 is used as an explant, and the test tube lotus root is induced to form a test-tube lotus after stem tip initiation, sub-generation proliferation, rooting and induction. Including the following steps:

[0022] 1. Stem apex start: take the cleaned and disease-free terminal buds of Elian No. 5, soak them in 75% alcohol for 30 seconds, and use 0.1% HgCl 2 Soak the solution for 3 to 12 minutes, and then rinse with sterile water 3 times for 3 to 5 minutes each time. After finishing the above surface disinfection treatment procedure, peel off the outer leaf sheath with a scalpel, cut the 0.3-0.6cm long stem tip, and inoculate it in the lotus root stem tip differentiation medium 1 / 2MS+6-BA2.0mg / L+NAA0.5mg / L+3.0% sugar, at a temperature of 24 to 26°C, a light intensity of 2000 to 2500 lx, light for 10 hours a day, and buds or clusters of buds will be produced after 30 to 45 days. The pH value of lot...

Embodiment 2

[0027] The apical buds of the Zilian variety Space 3 were used as explants, which were initiated by stem apex, proliferated, rooted, and induced to form a test tube lotus root. Including the following steps:

[0028] 1. Stem apex start: Take Space No. 3 as the test material, take clean and clean and disease-free Space No. 3 top buds, soak them in 75% alcohol for 30 seconds, and use 0.1% HgCl 2 Soak the solution for 3 to 12 minutes, and then rinse with sterile water 3 times for 3 to 5 minutes each time. After finishing the above surface disinfection treatment procedure, peel off the outer leaf sheath with a scalpel, cut the 0.3-0.6cm long stem tip, and inoculate it in the lotus root stem tip differentiation medium 1 / 2MS+6-BA2.0mg / L+NAA0.5mg / L+3.0% sugar, at a temperature of 24 to 26°C, a light intensity of 2000 to 2500 lx, light for 10 hours a day, and buds or clusters of buds will be produced after 30 to 45 days. The pH value of lotus root stem tip differentiation medium is 5.8...

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Abstract

The invention relates to an inducing technology of a test tube lotus root, which comprises the steps of: using terminal buds or lateral buds of the lotus root as explants; carrying out the starting of stem tips, successive proliferation and rooting; finally culturing and inducing by using a solid-liquid culture base to form the test tube lotus root. The method for inducing the test tube lotus root comprises the following steps of: cutting the stem tips of the lotus root and inoculating the stem tips on a stem tip starting culture base for culturing so as to grow up to become buds or a cluster of buds; transferring the buds or the cluster of buds to a successive culture base and carrying out successive proliferation; segmenting the cluster of buds into single bud and transferring to a rooting culture base and inducing to root to form tube seedlings; and rooting and culturing for 30d, pouring the liquid culture base into a culture container and inducing to form the test tube lotus root. In the process, the culture temperature is between 24 and 26 DEG C, the illumination intensity is 2000-25001x and the illumination is carried out for 10h every day. Through the stem tip culture and quick reproduction of the lotus root, the test tube lotus root is formed by inducing so as to quicken the reproduction speed of the lotus root, improve the transplant survival rate and industrially produce.

Description

Technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a test tube lotus root induction method. Background technique [0002] my country is one of the origin centers of lotus root. Lotus root is widely distributed and cultivated in my country. It is the largest aquatic vegetable cultivated in my country. It is very common in the Yellow River Basin and its south. With the adjustment of the rural industrial structure, the demand for lotus root species has increased sharply. However, because lotus root is a crop that reproduces mainly vegetatively, the reproduction coefficient is low (1∶10), and the reproduction speed of excellent and new lotus root species is far from meeting market demand. Moreover, conventional lotus root seeds have problems such as large amount of seeds, high transportation cost, large loss, and easy to carry diseases, which makes the development of lotus root industry subject to certain restrictions. Therefor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 柯卫东彭静刘玉平刘义满黄新芳叶元英李双梅李峰朱红莲黄来春赵春李明华
Owner WUHAN VEGETABLE RES INST
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