Culture medium and culture method for increasing yield of pleurotus eryngii

A culturing method and a technology for eryngium eryngii are applied in the directions of mushroom cultivation, botanical equipment and methods, cultivation, etc., can solve problems such as poor quality of strains, and achieve the effects of being conducive to popularization and application, low price, and increased yield

Inactive Publication Date: 2021-11-02
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to meet the needs of more people, the current production and planting technology of Pleurotus eryngii is relatively simple, and the quality of the strain is not good. It is necessary to adopt biological optimization technology to further improve the quality of Pleurotus eryngii

Method used

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  • Culture medium and culture method for increasing yield of pleurotus eryngii
  • Culture medium and culture method for increasing yield of pleurotus eryngii
  • Culture medium and culture method for increasing yield of pleurotus eryngii

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Effect test

Embodiment 1

[0036] The present embodiment provides a kind of culture medium and culture method that improves Pleurotus eryngii output, and concrete steps are as follows:

[0037] The first step is to prepare the fermentation medium: accurately weigh 30g / L of soybean powder, 1g / L of yeast extract, 30g / L of glucose, 0.5g / L of magnesium sulfate, 0.1g / L of iron sulfate, and 0.5g / L of potassium dihydrogen phosphate. L. Vitamin B10.01g / L, divide 80mL fermentation broth into 250mL Erlenmeyer flasks, sterilize in a sterilizer at 121°C for 30min, and use after cooling;

[0038] The second step, shake flask fermentation operation process: Passage and activate the mycelia of Pleurotus eryngii on a solid plate, and dig out 0.5×1.5cm in the solid plate with an inoculation shovel 2 The mycelium blocks with the same growth status were inserted into the above-mentioned culture medium;

[0039] The third step: set the pH to 5, 6, 7, 8, 9, and other conditions are the same, each batch is repeated 3 times,...

Embodiment 2

[0043] The present embodiment provides a kind of culture medium and culture method that improves Pleurotus eryngii output, and concrete steps are as follows:

[0044] The first step, prepare fermentation medium: with embodiment 1;

[0045] The second step, shake flask fermentation operation process: with embodiment 1;

[0046] Step 3: Set the pH to 6, set the temperature to 21°C, 23°C, 25°C, 27°C, 29°C, and other conditions are the same, each batch is repeated 3 times, and the shaker is shaken for 7 days;

[0047] The 4th step, thalline biomass determination: with embodiment 1;

[0048] Step 5: The final test found that when the temperature was 27°C, the biomass of Pleurotus eryngii reached the maximum value, which was 11.86g / L.

Embodiment 3

[0050] The present embodiment provides a kind of culture medium and culture method that improves Pleurotus eryngii output, and concrete steps are as follows:

[0051] The first step, prepare fermentation medium: with embodiment 1;

[0052] The second step, shake flask fermentation operation process: with embodiment 1;

[0053] Step 3: Set the pH to 6, the temperature to 27°C, and the liquid volume to 80mL, 90mL, 105mL, 120mL, and 135mL. Other conditions are the same. Each batch is replicated 3 times, and cultured on a shaking table for 7 days;

[0054] The 4th step, thalline biomass determination: with embodiment 1;

[0055]The fifth step: the final test found that when the liquid volume was 90mL, the biomass of Pleurotus eryngii reached the maximum value, which was 12.26g / L.

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Abstract

The invention discloses a culture medium and a culture method for increasing the yield of pleurotus eryngii. The culture medium is prepared from the following raw materials in parts by mass: 3 parts of soybean meal, 0.1 part of yeast extract, 3 parts of glucose, 0.05 part of magnesium sulfate, 0.01 part of ferric sulfate, 0.05 part of monopotassium phosphate, 0.001 part of vitamin B1 and 100 parts of water. The method comprises the following steps of pleurotus eryngii hyphae are subjected to passage activation in a solid plate, and hypha blocks with consistent growth conditions are dug from the solid plate by using an inoculation shovel and inoculated into the culture medium; pH, temperature, liquid loading amount and rotating speed are set, and shaking culture is performed on a shaking table for 7 days; all fermentation liquor is filtered by using a 40-mesh screen, supernate is filtered out, vacuum suction filtration is carried out by using weighed qualitative filter paper, the supernate is filtered out, mycelium is washed with water for 3 times, drained, collected, dried in an air dry oven and weighed to constant weight, and the operation is repeated for 3 times; by adopting the fermentation conditions, the strain grows quickly under the process, and the quality of the pleurotus eryngii can be improved.

Description

technical field [0001] The invention relates to the technical field of Pleurotus eryngii cultivation, in particular to a culture medium and a cultivation method for improving the yield of Pleurotus eryngii. Background technique [0002] Pleurotus eryngii has high nutritional value, and its protein content is as high as 25%. It contains a variety of amino acids and polysaccharides, and has a variety of biological activities, such as anti-cancer and anti-cancer and immunomodulatory activities. At the same time, it contains a large number of small molecular natural products, such as sterols, which have good cytotoxicity, anti-osteoporosis, antioxidant and anti-inflammatory effects. [0003] The flesh of Pleurotus eryngii mushroom is thick, crisp and tender, especially the stipe is dense, firm and milky white, and can be eaten entirely, and the stipe is more crispy and refreshing than the cap. ", with a pleasant almond aroma and abalone-like taste, suitable for preservation and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20
CPCA01G18/00A01G18/20
Inventor 刘长青冯嘉洁王思敏曹逸申玉香张银文黄应瑞丁兵发
Owner YANCHENG INST OF TECH
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