Domestication method of full suspension culture-type MDCK cell line
A full-suspension, cell line technology, applied in the field of cell culture, can solve the problems of inability to meet the needs of virus vaccine production, unsuitable for large-scale culture, and decreased virus yield, etc., to facilitate large-scale production, reduce site and labor input, reduce The effect of the chance of contamination
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment
[0030] 1. Experimental materials Frozen adherent cultured MDCK cell line, DMEM, F12, RPMI1640 medium, 0.25% EDTA trypsin, trypan blue staining solution, cell square flask, cell shaker flask, pipette.
[0031] 2. Instruments and equipment CO 2 Incubators, orbital shakers, inverted microscopes, Countstar cell counters, low speed refrigerated centrifuges.
[0032] 3. Method
[0033] 3.1 Acclimatization of MDCK cells in serum-free suspension
[0034] Step 1. Mix DMEM, F12 and RPMI1640 according to the ratio of 3:1:1, and use this as the basal medium, add 12%, 8%, 5%, 3%, 1%, 0% fetal bovine serum respectively Configured as DMEM-F12-RPMI1640 medium with serum or DMEM-F12-RPMI1640 medium without serum;
[0035]Step 2. Resuscitate an adherent cultured MDCK cell, inoculate and culture it with DMEM-F12-RPMI1640 containing 12% fetal bovine serum as described in step 1. The culture condition is 37°C, 5% CO 2 , pH7.2; after the cells adhered to the wall and the confluence of the monol...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com