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Saccharomyces cerevisiae and application thereof to fermentative production of S-adenosylmethionine

A technology of adenosylmethionine and Saccharomyces cerevisiae strains, which is applied in the field of microorganisms, can solve the problems of increasing the burden of S-adenosylmethionine production condition control, affecting the production of S-adenosylmethionine, etc., achieving a clear and simple process, easy to control, and simple culture conditions Ease of control and increased output

Active Publication Date: 2017-11-07
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of fermenting and producing S-adenosylmethionine, the original Saccharomyces cerevisiae strains have strict requirements on the control of temperature and pH, which seriously affects the production of S-adenosylmethionine, and increases the production condition control of S-adenosylmethionine. big burden

Method used

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  • Saccharomyces cerevisiae and application thereof to fermentative production of S-adenosylmethionine

Examples

Experimental program
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Effect test

Embodiment 1

[0031] The inventor used low-energy ion beam implantation technology to screen the Saccharomyces cerevisiae that can obtain the product S-adenosylmethionine under the induction of L-methionine in the soil; at 30°C, the screened Saccharomyces cerevisiae Inoculate into YPD culture medium and cultivate for 4 hours, centrifuge to remove the supernatant, add appropriate amount of normal saline to make S. After dilution, the strained bacterial suspension was spread on the YPD solid medium for culture; a single strain with good growth was selected and inoculated in the liquid fermentation medium for culture, and after three rounds of screening, the stable genetic traits capable of high-yielding S-adenosine were selected. Saccharomyces cerevisiae strains of methionine, that is, Saccharomyces cerevisiae strains with high production of S-adenosylmethionine.

Embodiment 2

[0033]Plate medium: 20 g / L peptone, 10 g / L yeast extract, 20 g / L glucose, 20 g / L agar, natural pH.

[0034] Aerobic fermentation medium: glucose 37.5 g / L, corn steep liquor 45 g / L, malt powder 15 g / L, K2HPO4•3H2O 1.6 g / L, KH2PO4 1.25 g / L, MgSO4•7H2O 3.75 g / L, initial pH 5.0. 500mL Erlenmeyer bottle with a volume of 30mL, sterilized at 121°C for 15 minutes.

[0035] The initial strain and CGMCC No. 13760 bacteria were firstly activated on the plate at 30°C, and after 24 hours, two rings of well-grown bacteria were put into 30mL fermentation medium, cultured at 30°C, 200rpm for 12h, and then 3% (v / v ) the inoculum amount is transferred to a 30ml fermentation broth / 500ml Erlenmeyer flask, cultured at 30°C, and the rotation speed is 200rpm. After the bacteria grow to the mid-logarithmic growth phase (that is, fermentation for 48 hours), add 0.1 g of D / L-methionine to the fermentation broth. After 30 hours of fermentation, the fermentation broth of CGMCC No. 13760 contained 2.95g...

Embodiment 3

[0037] Plate culture medium: with embodiment 1;

[0038] Aerobic fermentation medium: glucose 60g / L, corn steep liquor 10g / L, malt extract powder 5g / L, yeast powder 15g / L, acidified molasses 40 g / L, K2HPO4•3H2O1.6 g / L, KH2PO4 1.25 g / L, MgSO4•7H2O 3.75 g / L, FeSO4•7H2O 10mg / L, (NH 4 ) 2 HPO 4 5 g / L, initial pH 5.0. 7 L fermenter with 3 L volume, sterilized at 121°C for 15 minutes.

[0039] The initial strain and CGMCC No.13760 bacteria were firstly activated on the plate at 30°C, and after 24 hours, two rings of well-grown bacteria were put into 30mL fermentation medium, cultivated at 30°C, 200rpm for 12h, and then according to 10% (v / v ) inoculum amount was transferred into 3L fermentation medium, ventilated with air at 2v / vm, ​​rotated at 800rpm, and cultured at 30°C. After 16 hours of fermentation, start to add glucose, and control the concentration of glucose to 10-15g / L. After the bacteria grow to the mid-logarithmic growth stage (ie, 36 hours of fermentation), add 10...

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Abstract

The invention discloses saccharomyces cerevisiae and application thereof in the fermentative production of S-adenosylmethionine. The strain of saccharomyces cerevisiae is classified and named as (saccharomyces cerevisiae) ty-93620 and is preserved in China Microbial Culture Collection Administration General Microbiology Center, with the preservation number of CGMCC No. 13760. The invention also discloses a method for producing S-adenosylmethionine by utilizing the saccharomyces cerevisiae strain capable of achieving high production of S-adenosylmethionine. Compared with a chemical method, the method disclosed by the invention has the advantages of being low in production cost, low in energy consumption, free of pollution, free of crude raw materials, such as non-fossil resources, as raw materials, free of ATP as a catalyst inducer, high in yield, strong in stability, clear and simple in process and easy to operate. Compared with original bacteria, the strain in the invention has fewer types of fermentation products, high purity, good stability and obviously increased yield.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Saccharomyces cerevisiae strain with high yield of S-adenosylmethionine and its application in fermentative production of S-adenosylmethionine. Background technique [0002] S-adenosyl-methionine (SAM for short) is an important metabolic intermediate widely present in organisms and an activated form of methionine (Methionine, Met). It was discovered by Cantoni in 1952. In biology, S-adenosylmethionine is an important methyl donor, which has three functions of transmethylation, transsulfurization and transaminopropylation. Clinically, S-adenosylmethionine was used as an anti-inflammatory painkiller for treating arthritis in Europe in the late 1970s, as a psychotropic drug for antidepressant in Europe in the mid-1980s, and as an antidepressant and liver protection drug in the United States in the 1990s. Over-the-counter drugs and nutraceuticals have been used ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/18C12P19/40C12R1/865
CPCC12P19/40C12R2001/865C12N1/185
Inventor 郝宁谭玉岩郭格格刘兆星
Owner NANJING TECH UNIV
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