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Induced culture method of tender shoots of aronia melanocarpa

The technology of a kind of Sorbus nigrutica and its cultivation method is applied in horticultural methods, botanical equipment and methods, horticulture, etc., and can solve the problems such as the shortage of seedlings restricting the development of Sorbus nigrutica, and achieve the benefits of popularization and production, The effect of simple materials and controllable culture conditions

Inactive Publication Date: 2018-05-11
JILIN AGRI SCI & TECH COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many farmers and enterprises planting Sorbus black fruit in Northeast China, and the number of high-quality seedlings required is estimated to be more than tens of millions. The seedlings provided by ramets and cuttings cannot meet market demand, and the shortage of seedlings restricts Sorbus sago Catalpa industry development

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for inducing and cultivating young shoots of Arana nigruta, comprising the steps of:

[0021] The Sorbus adenocarpus plant to be multiplied is selected as the mother tree for collecting explants, and the 20 cm long branch of the top tip is cut during the growth period, rinsed under running water, cut into 3 cm long sections, and placed in an ultra-clean workbench , under aseptic conditions, cut the young shoots, with less branch xylem, disinfect in alcohol for 20 seconds, then soak in glutaraldehyde solution for 10 minutes, rinse with sterile water, inoculate into the medium, MS+sucrose 30g / L+agar powder 6g / L+6-BA (6-benzylpurine) 2.0g / L+NAA (naphthaleneacetic acid) 0.2g / L, induction culture was carried out in an intelligent incubator for 20 days.

Embodiment 2

[0023] A method for inducing and cultivating young shoots of Arana nigruta, comprising the steps of:

[0024] Select the Sorbus adenocarpus plant to be multiplied as the mother tree for collecting explants, cut 1-year-old branches with a length of 30 cm during the dormant period, and move them into the greenhouse for water insertion culture. The plastic film canopy is used to keep heat and keep moisture, and wait for the new buds to germinate Then cut it off, rinse it under running water, immerse it in sterile water and put it in an ultra-clean workbench. Under aseptic conditions, peel off the young shoots, with less xylem, disinfect them in alcohol for 20 seconds, and then put them in glutaraldehyde solution Soak in medium for 10 minutes, rinse with sterile water, inoculate into the culture medium, MS + sucrose 30g / L + agar powder 6g / L + 6-BA (6-benzylpurine) 2.0g / L + NAA (naphthaleneacetic acid) 0.2g / L , and induced culture for 20 days in an intelligent incubator.

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Abstract

The invention discloses an induced culture method of tender shoots of aronia melanocarpa. The method comprises steps as follows: a to-be-cultured aronia melanocarpa plant is selected, healthy and strong twigs free from disease and pest damage are sheared in the growing period and subjected to asepsis treatment, an induced culture medium is inoculated with the twigs, induced culture is performed under the proper light and temperature conditions, sterile sprouts in vitro are formed through callus induction and induction of clustered shoots, a primary culture system under the sterile condition isestablished, and a base material is provided for next subculture. The materials are simple and easy to obtain, each link is easy to operate, the culture conditions are controllable, the repeatabilityof result is high, and the method is low in cost, favorable for popularization and input production and has great practical significance for promoting rapid propagation of good seedlings of aronia melanocarpa.

Description

technical field [0001] The invention relates to the fields of agriculture and plant cultivation, in particular to a method for aseptically inducing clustered buds of Aroniame Lanocarpa. Background technique [0002] Sorbus adenocarpa is a tree species of the genus Aronia in the family Rosaceae. It is a tree species introduced from abroad. m, bushy tree shape, used for landscaping and berry cultivation. Sorbus nigra is suitable for the cold winter climate in Northeast China. In recent years, the cultivation area has been continuously expanding, and there is a shortage of seedlings, especially pure varieties. The rapid production of seedlings by plant tissue culture technology is the best choice to solve the problem of large demand for seedlings and insufficient reproduction in a short period of time. Due to the relatively short time of introduction of Sorbus nigra in China, the practical technology of group cultivation of seedlings is still in a blank state, especially the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 鞠志新陈君史春凤范文忠
Owner JILIN AGRI SCI & TECH COLLEGE
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