A kind of preparation method of notoginseng seed

A technology of Panax notoginseng seeds and Panax notoginseng, which is applied in the fields of botanical equipment and methods, gardening methods, gardening tools/equipment, etc., can solve the problems of easy browning, long production cycle, shortage of seedlings, etc. The effect of overcoming easy browning and stable character

Active Publication Date: 2020-11-24
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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Problems solved by technology

[0007] The object of the present invention is to provide a kind of preparation method of Panax notoginseng seeds, solve the situation of shortage of seedlings in the large-scale artificial cultivation of Panax notoginseng by utilizing modern biotechnology, the embryogenic cells of Panax notoginseng have the advantages of fast growth rate and stable character, overcome It solves the defects of easy browning, variation, long production cycle and high cost in the conventional tissue culture of Sanqi, and also solves the problems of less seeds and low reproduction rate of Sanqi, and opens up a new way for the cultivation of Panax notoginseng seeds. Realized the proliferation and differentiation of Panax notoginseng somatic embryos, and the germination rate of the finally prepared Panax notoginseng artificial seeds can reach more than 95%

Method used

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  • A kind of preparation method of notoginseng seed
  • A kind of preparation method of notoginseng seed
  • A kind of preparation method of notoginseng seed

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Experimental program
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Embodiment 1

[0037] The production method of Panax notoginseng artificial seeds is as follows:

[0038] (1) Proliferation of somatic embryos: connect the embryogenic callus to the proliferation medium, the medium consists of: SH+0.2mg / L 2,4-D+28g / L sucrose+5g / L agar, each Subculture once every 4 weeks on the same medium, and culture in dark at 24°C. Maintain the differentiation ability of embryogenic callus;

[0039] (2) Differentiation of somatic embryos: insert the embryogenic callus onto the somatic embryo differentiation medium, the medium composition is: SH+32g / L sucrose+7g / L agar, culture in dark, culture temperature is 30°C . Obtain mature cotyledon-shaped embryos (about 4mm in length) after 50 days;

[0040](3) Production of Panax notoginseng artificial seeds: use Panax notoginseng somatic embryos of about 4mm as embedded propagules, SH+1% sucrose as artificial endosperm, and 3wt% sodium alginate as artificial seed coat matrix. Artificial seed production by infusion method: on ...

Embodiment 2

[0042] The production method of Panax notoginseng artificial seeds is as follows:

[0043] (1) Proliferation of somatic embryos: connect the embryogenic callus to the proliferation medium, the medium consists of: SH+0.4mg / L 2,4-D+32g / L sucrose+8g / L agar, each Subculture once in the same medium every 6 weeks, and culture in dark at 20°C. Maintain the differentiation ability of embryogenic callus;

[0044] (2) Differentiation of somatic embryos: insert the embryogenic callus onto the somatic embryo differentiation medium, the medium composition is: SH+28g / L sucrose+5g / L agar, culture in dark, culture temperature is 35°C . After 50 days, mature cotyledon-shaped embryos (about 6 mm in length) were obtained;

[0045] (3) Production of Panax notoginseng artificial seeds: use Panax notoginseng somatic embryos of about 6 mm as embedded propagules, SH+1% sucrose as artificial endosperm, and 3wt% sodium alginate as artificial seed coat matrix. Artificial seed production by infusion ...

Embodiment 3

[0047] The production method of Panax notoginseng artificial seeds is as follows:

[0048] (1) Proliferation of somatic embryos: connect the embryogenic callus to the proliferation medium, the medium consists of: SH+0.3mg / L 2,4-D+30g / L sucrose+7g / L agar, each Subculture once every 5 weeks on the same medium, and culture in dark at 22°C. Maintain the differentiation ability of embryogenic callus;

[0049] (2) Differentiation of somatic embryos: insert the embryogenic callus onto the somatic embryo differentiation medium, the medium composition is: SH+30g / L sucrose+6g / L agar, culture in dark, culture temperature is 32°C . After 55 days, mature cotyledon-shaped embryos (about 5 mm in length) were obtained;

[0050] (3) Production of Panax notoginseng artificial seeds: Somatic embryos of Panax notoginseng with about 5 mm were used as embedding propagules, SH+1% sucrose was used as artificial endosperm, and 3wt% sodium alginate was used as artificial seed coat matrix. Artificia...

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Abstract

The invention provides a preparation method of Panax notoginseng seeds, comprising the following steps: differentiating and culturing the embryogenic callus of Panax notoginseng to obtain somatic embryos, and the composition of the culture medium is: SH+0.2-0.4mg / L 2,4-D +28‑32g / L sucrose+5‑8g / L agar; the somatic embryos are further differentiated and cultured to obtain mature cotyledon-shaped embryos. The medium composition is: SH+28‑32g / L sucrose+5‑7g / L agar; The cotyledon-shaped embryo is used as the embedded propagule, SH+1-2wt% sucrose is used as the artificial endosperm, and 3-5wt% sodium alginate is used as the artificial seed coat base, and the artificial seed is produced by dripping method. The preparation method of Panax notoginseng seeds in the embodiment of the present invention solves the shortage of seedlings in the large-scale artificial cultivation of Panax notoginseng by using modern biotechnology, and the embryogenic cells of Panax notoginseng have the advantages of fast growth and stable properties.

Description

technical field [0001] The invention relates to the field of notoginseng cultivation, in particular to a preparation method of notoginseng seeds. Background technique [0002] Panax notoginseng, also known as Tianqi, is an Umbelliferae Araliaceae plant, mainly distributed in Yunnan, Guangxi, Jiangxi, Sichuan and other places. Chinese herbal medicine Panax notoginseng uses its root as a medicinal part, which has the effects of dispelling blood stasis, stopping bleeding, reducing swelling and relieving pain. Indications for hemoptysis, hematemesis, epistaxis, hematochezia, metrorrhagia, traumatic bleeding, chest and abdomen tingling, tumbling pain. [0003] Panax notoginseng is a traditional precious Chinese herbal medicine in my country, one of the 40 kinds of large medicinal materials. It has a history of more than 400 years of application and has a long-standing reputation at home and abroad. Sanqi has been known as the "hemostatic medicine" since ancient times. Due to th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/006
Inventor 雷秀娟郑惠元宋娟姚丽娜王英平
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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