MD2 pineapple rooting culture medium and tissue culture seedling rapid propagation method thereof

A rooting medium and technology for rooting culture, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as inability to meet large-scale planting of seedlings, instability of excellent genetic traits, differences in tissue culture methods, etc. Achieve the effect of solving the shortage of seedlings, improving the survival rate of transplanting, and increasing the reproduction speed.

Inactive Publication Date: 2017-09-29
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are some reports on tissue culture of other pineapple varieties, but due to the large differences in genotypes between different varieties, there are large differences in tissue culture methods between different varieties
MD2 pineapple relies on the traditional vegetative propagation method. Its growth rate is slow and its reproduction coefficient is low. It cannot meet the needs of large-scale planting seedlings in a short period of time. Mother plant pests and diseases passed on to the next generation

Method used

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  • MD2 pineapple rooting culture medium and tissue culture seedling rapid propagation method thereof
  • MD2 pineapple rooting culture medium and tissue culture seedling rapid propagation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] An MD2 pineapple rooting medium, the MD2 pineapple rooting medium comprises 1 / 2MS+IBA0.5mg / L+paclobutrazol 1.0mg / L+sucrose 20g / L+agar 6.0g.

[0020] Utilize the MD2 pineapple tissue culture seedling rapid propagation method of above-mentioned MD2 pineapple rooting medium, comprise the following steps:

[0021] (1) Selection and disinfection of explants: select MD2 pineapple sucking buds growing robustly in fine weather, remove the leaves around the top meristem, sterilize with 0.1% mercuric chloride solution for 15 minutes, and then rinse with sterile water for 3 blot the remaining moisture on the surface with dry sterile paper towels.

[0022] (2) Suction bud pre-cultivation: longitudinally cut the apical meristem in step (1) into 2 uniform pieces, inoculate in MS+6-BA3.0mg / L+IBA0.2mg / L+sucrose 30g / L+agar 6.0g The solid medium was adjusted to a pH value of 5.5, cultured at 25°C for 10 days, and illuminated for 12 hours a day with a light intensity of 2000 lx.

[0023...

Embodiment 2

[0027] An MD2 pineapple rooting medium, the MD2 pineapple rooting medium comprises 1 / 2MS+IBA1.0mg / L+paclobutrazol 2.0mg / L+sucrose 20g / L+agar 7.0g.

[0028] Utilize the MD2 pineapple tissue culture seedling rapid propagation method of above-mentioned MD2 pineapple rooting medium, comprise the following steps:

[0029] (1) Selection and disinfection of explants: select MD2 pineapple sucking buds growing robustly in fine weather, remove the leaves around the top meristem, sterilize with 0.1% mercuric chloride solution for 20 minutes, and rinse with sterile water for 5 minutes. blot the remaining moisture on the surface with dry sterile paper towels.

[0030] (2) Suction bud pre-cultivation: longitudinally cut the apical meristem in step (1) into 2 uniform pieces, inoculate in MS+6-BA3.0mg / L+IBA0.2mg / L+sucrose 30g / L+agar 7.0g The solid medium was adjusted to a pH value of 6.0, cultured at 30°C for 15 days, and illuminated for 14 hours a day with a light intensity of 3000 lx.

[...

Embodiment 3

[0035]An MD2 pineapple rooting medium, the MD2 pineapple rooting medium comprises 1 / 2MS+IBA0.8mg / L+paclobutrazol 1.5mg / L+sucrose 20g / L+agar 6.5g.

[0036] Utilize the MD2 pineapple tissue culture seedling rapid propagation method of above-mentioned MD2 pineapple rooting medium, comprise the following steps:

[0037] (1) Selection and disinfection of explants: select MD2 pineapple sucking buds growing robustly in sunny weather, remove the leaves around the top meristem, sterilize with 0.1% mercuric chloride solution for 18 minutes, and rinse with sterile water for 4 minutes. blot the remaining moisture on the surface with dry sterile paper towels.

[0038] (2) Suction bud pre-cultivation: longitudinally cut the apical meristem in step (1) into 2 uniform pieces, inoculate in MS+6-BA3.0mg / L+IBA0.2mg / L+sucrose 30g / L+agar 6.5g The solid medium was adjusted to a pH value of 5.8, cultured at 28°C for 10 days, and illuminated for 13 hours a day with a light intensity of 2500 lx.

[...

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Abstract

The invention discloses a MD2 pineapple rooting culture medium and a tissue culture seedling rapid propagation method thereof. The MD2 pineapple rooting culture medium contains paclobutrazol. The MD2 pineapple tissue culture seedling rapid propagation method comprises the following steps of: firstly, selecting a MD2 pineapple water sprout and disinfecting the same; and then performing pre-culture, multiplication culture, rooting culture and transplanting thereon, wherein the rooting culture medium is 0.5-1.0mg/L 1/2MS+1BA+1.0-2.0mg/L paclobutrazol+20g/L saccharose+6.0-7.0g of agar, and the pH value is 5.5-6.0. The MD2 pineapple seedling is rapidly propagated by way of tissue culture; in the MD2 pineapple seedling rooting culture, after the paclobutrazol is added, the roots are more orderly and more robust, and are better to absorb nutrients; photosynthesis of blades is enhanced, the lobus cardiacus thickness and hardness are increased, water of the blades can be better kept after transplanting, invasion of bacteria and fungi is resisted, the stress resistance of the tissue culture seedling is enhanced, and the transplanting survival rate is also increased to a relatively great extent; rooting culture can be performed by using natural light, so that electric energy is saved; and the rooting seedling cultured under natural lighting can be directly transplanted to a nursery for temporary planting, so that the manual cost is lowered.

Description

technical field [0001] The invention relates to the technical field of pineapple seedling cultivation, in particular to an MD2 pineapple rooting medium and a method for rapid propagation of tissue culture seedlings thereof. Background technique [0002] The weight of MD2 golden pineapple is generally 1-2 kg, cylindrical, with no thorns on the leaf edge, slender leaves with smooth surface, and the number of leaves is 10% more than other varieties, with thin peel and shallow flower cavity. The pulp is golden yellow, fine in texture, medium in fiber, dense in taste, melts in the mouth, rich in flavor and good in taste. In particular, the skin is thin and there is no need to cut the skin, and the sweet taste does not need to be soaked in salt water. It can be eaten directly after peeling, which is very convenient to eat. MD2 golden pineapple is fragrant and juicy, and has a long storage period. If it is stored at 7.5 degrees Celsius, it can be stored for up to 25 days. Therefor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 刘兴地胡兰莫坤联王向社李明芳郑学勤
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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