The invention discloses a high-resolution
biosensor, which includes an atomic-scale conductive material as a sensitive unit to achieve atomic-level detection resolution and a micro-nano fluid device to control the motion and morphological structure of detected molecules. The second electrophoretic
electrode or
micropump, the second storage room, the second micro-nano separation channel, the substrate, the first insulating layer, the sensitive functional layer, the second insulating layer, the first micro-nano separation channel, the first storage room, the first The electrophoretic electrodes or micropumps are placed in sequence, the center of the sensitive functional layer is provided with nanopores, the center of the first insulating layer is provided with openings in the first insulating layer, the center of the second insulating layer is provided with openings in the second insulating layer, and the substrate A substrate opening is arranged in the center of the substrate, and an electrical
contact layer for measuring electrical signals is arranged on the sensitive functional layer. The sensitive functional layer with an atomic
layer thickness of the present invention enables the resolution of the sensor to reach the atomic scale, and the integration with the micro-nano fluidic device can control the movement and structure of
DNA or
RNA, so that stable
signal detection can be obtained.