The invention belongs to the field of biomedical materials, and particularly relates to photo-curing hydrogel for multi-
cell sorting and
stem cell area-selecting differentiation and a preparation method thereof. The photo-curing hydrogel is prepared by taking modified high-
polymer materials with the photo-curing performance as raw materials and are divided into multiple different areas by straight-stripe-shaped or net-stripe-shaped or annular-stripe-shaped micro-pattern structures, the
hardness of the photo-curing hydrogel in the same area is same, the
hardness of the photo-curing hydrogel in the different areas is same or different, and the overall photo-curing hydrogel at least has two kinds of the
hardness. According to the photo-curing hydrogel for multi-
cell sorting and
stem cell area-selecting differentiation and the preparation method thereof, on one hand, the elasticity moduli of all the areas of the photo-curing hydrogel are different by controlling the
ultraviolet radiation curing degree; on the other hand, all the areas with the different elasticity moduli present the micro-pattern structures on the photo-curing hydrogel, selective adhesion, proliferation and differentiation of cells on the photo-curing hydrogel can be achieved by combining the elasticity moduli with the micro-pattern structures, and therefore controllable
cell sorting or
stem cell area-selecting differentiation can be achieved.