Gel injection combining molecular targeted drug and cytotoxic drug
A technology of molecular targeted drugs and gel injections, which is applied in the direction of drug combinations, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., to achieve low toxicity, high therapeutic effect, and good stability
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Embodiment 1
[0052] Embodiment 1, preparation of lapatinib microparticles and paclitaxel nanoparticle gel injection
[0053] (1) Preparation of lapatinib microparticle suspension: Weigh 60 mg of lapatinib and dissolve it in 1 ml DMSO, take 200 μl, and inject the drug solution into distilled water under ice bath under the condition of ultrasonic probe to prepare After the obtained medicinal solution is suction-filtered, it is re-suspended with an appropriate amount of distilled water (final concentration is 8 mg / ml), and ultrasonically dispersed with a short-term probe to obtain a lapatinib microparticle solution.
[0054] (2) Preparation of Paclitaxel Nanoparticles Suspension: Weigh 32mg Paclitaxel and 160mg Pluronic F127 respectively, add 4ml of chloroform, and ultrasonically dissolve it completely, blow the organic solvent to dryness by nitrogen at a constant flow rate, and place 25°C in a vacuum oven for 12 hours to remove residual organic solvents. Add 8ml of deionized water to the dr...
Embodiment 2
[0056] Embodiment 2, the preparation of imatinib liposome and Taxol gel injection
[0057] (1) Preparation of imatinib liposomes: take a certain amount of lipid material (EPC: Chol: DSPE-PEG2000 = 57:38:5, m / m / m) dissolved in chloroform, and at 37 ° C Vacuum rotary evaporation film formation. Afterwards, a certain amount of 300 mM ammonium sulfate solution was added for hydration, and a water bath was sonicated at 37° C. for 20 min to prepare blank liposomes. Pass through the column to remove free ammonium sulfate, and then incubate with a certain amount of imatinib (lipid material: imatinib=15:1, w / w) at 37°C for 60 min, pass the unencapsulated free drug to remove . Prepare a final concentration of 2 mg / ml.
[0058] (2) It is a commercial preparation of PTX, the concentration is 6mg / ml, and the solvent is ethanol:polyoxyethylene castor oil=1:1.
[0059] (3) Preparation of mixed drug gel injection: Weigh 0.5g Pluronic F127, add to 1ml imatinib liposome solution, 0.5ml ...
Embodiment 3
[0060] Example 3, Preparation of lapatinib and camptothecin mixed polyelectrolyte nanocapsule gel injection
[0061] (1) Preparation of lapatinib and camptothecin mixed polyelectrolyte nanocapsules: camptothecin (40mg), chitosan (6mg) and ammonium bicarbonate (40mg) were added to 30ml of distilled water, stirred for 5min, and cooled The mixed nano-core of camptothecin / chitosan was prepared by ultrasonication in a water bath for 45 min. Then add 4ml of alginic acid (1mg / ml) and sonicate for 25min. Add chitosan and alginic acid solution repeatedly 3 times to prepare camptothecin / (chitosan / alginic acid) 3 Composition, then successively add 20ml of lapatinib (0.5mg / ml) and alginic acid solution under continuous ultrasound to prepare camptothecin / (chitosan / alginic acid) 3 / -lapatinib / alginic acid sample, centrifuge at 10000rpm at high speed for 10min, remove the supernatant, add appropriate amount of solvent to resuspend to an appropriate concentration (camptothecin 4mg / ml, lapat...
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