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34 results about "Protein need" patented technology

How much protein you need depends on a few factors, but one of the most important is your activity level. The basic recommendation for protein intake is 0.8 grams per kilogram (or around 0.36 g per pound) of body mass in untrained, generally healthy adults.

Gadus dried meat floss cake

A gadus dried meat floss cake comprises, by weight, 180-190 parts of wheat flour, 30-40 parts of lotus root starch, 40-50 parts of rice flour, 10-20 parts of black soybean flour, 20-30 parts of konjaku flour, 8-10 parts of yeast powder, 50-60 parts of egg, 40-50 parts of codfish, 10-20 parts of eel meat, 50-60 parts of dried beef floss, 4-5 parts of calculus bovis, 6-7 parts of pearl, 5-6 parts of albizzia flower, 4-5 parts of radices hemerocallis, 5-6 parts of marigold, 4-5 parts of konjak leaves, 40-50 parts of yellow rice or millet wine, 20-30 parts of white vinegar, 10-20 parts of fresh ginger slice, 10-60 parts of white granulated sugar and appropriate water. According to the gadus dried meat floss cake, a plurality of kinds of powders such as the konjaku flour and the lotus root starch are used as the cake pig trotter materials, nutrition is abundant, the flavor is good, gadus and other meat containing animal protein are added, and protein needed by the human body can be supplemented. Meanwhile, albizzia flower and other extracts of Chinese herbal medicines with the function of soothing the nerves are especially added, and therefore the gadus dried meat floss cake has the very good function of soothing the nerves and is suitable for most people.
Owner:重庆华仁实业有限公司

Ecologically balanced and nutritional adult turtle feed formula

InactiveCN104273384ABalanced nutritional intakeSame nutritional costAnimal feeding stuffVitamin B12Pantothenic acid
The invention discloses an ecologically balanced and nutritional adult turtle feed formula. The mass ratios of the raw materials are as follows: 41-50 parts of white fishmeal, 19-30 parts of red fishmeal, 3-5 parts of corn protein powder, 1-3 parts of fermented soybean meal, 1-4 parts of wheat gluten powder, 2-4 parts of beer yeast, 2-5 parts of soybean protein concentrate, 15-30 parts of starch, 0.1-0.2 part of calcium dihydrogenphoshate, 2-4 parts of premix and 0.2-0.4 part of phytoxanthin; the mass ratios of the raw materials of the multidimensional premix are as follows: 5000-6000 parts of vitamin A, 2100-3200 parts of vitamin D, 85-165 parts of vitamin E, 8-17 parts of vitamin K, 19-28 parts of vitamin B1, 17-33 parts of vitamin B2, 22-35 parts of vitamin B6, 0.06-0.15 part of vitamin B12, 190-330 parts of nicotinic acid, 28-44 parts of D-pantothenic acid, 4-16 parts of folic acid, 220-260 parts of biotin and 270-360 parts of inositol. The ecologically balanced and nutritional adult turtle feed formula disclosed by the invention can be used for effectively supplementing various minerals, trace elements and protein needed in the growth process of turtles, and can be used for ensuring that the artificial feeding turtles have similar nutritional value with that of the wild turtles and ensuring the cooking taste of the artificial feeding turtles same as that of the wild turtles.
Owner:ZHEJIANG JINDADI FEED

Enzymatic hydrolysis peptide map analysis method for polyethylene glycol modified protein medicine

The invention relates to a peptide map analysis method for polyethylene glycol modified protein. According to the method, firstly, a high-order structure of the protein needs to be destroyed to fullyexpose restriction enzyme cutting site, and then proteolysis is carried out; secondly, by selecting an appropriate chromatographic packing aperture, the peptide map analysis method with ideal analysisperformance for PEG-modified proteins is obtained. Through effective use of the method, a PEG-modified peptide segment in a peptide map can be effectively detected, and the technical problem that modified protein and unmodified protein cannot be distinguished by conventional methods is solved.
Owner:ZONHON BIOPHARMA INST

Method for fixing protein on surface of polydimethylsiloxane

The invention provides a method for fixing a protein on the surface of polydimethylsiloxane. The method is characterized in that a biotinylated polydimethylsiloxane film is prepared on the solidified surface of polydimethylsiloxane, a protein is fixed on the surface of polydimethylsiloxane by chemical technologies of biotin-avidin and water-soluble carbodiimide and a technology of 3-mercaptopropionic acid stabilized gold nano-particles, and beta-D-dodecyl-N-maltose and bovine serum albumin are utilized respectively for blockading the non-specific adsorption of a protein on the surface of polydimethylsiloxane and activated residual carboxylic groups on the surface of a 3-mercaptopropionic acid stabilized gold nano-particle. In the invention, the surface of a 3-mercaptopropionic acid stabilized gold nano-particle contains multiple carboxylic groups which can combine molecules of multiple proteins needing to be fixed thus multiple proteins needing to be fixed can be fixed on the surface of polydimethylsiloxane and a sensitivity of a subsequent enzyme-linked immuno sorbent assay (ELISA) is improved effectively.
Owner:SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY

Food formula beneficial to weight control and reduction of three highs (high blood pressure, high blood fat and high sugar)

The invention discloses a food formula benficial to weight control and reduction of three highs (hihg blood pressure, high blood fat and high sugar). The food formula comprises, by weight, 18-22 partsof inulin, 16-20 parts of whey protein powder, 10-12 parts of skimmed milk powder, 9-11 parts of soybeam dietary fiber, 9-11 parts of oat meal, 5.4-5.8 parts of maltodextrin, 2.5-3.5 parts of maca powder, 1.8-2.2 parts of barley leave green juice, 1.3-1.7 parts of konjac powder, 1.4-1.6 parts of psylliumseedhusk, 1.4-1.6 parts of potassium citrate, 0.4-0.6 part of chia seeds, 0.03-0.05 part of stevioside, 0.4-0.6 part of concentrated vanilla and 0.3-0.5 part of fresh milk flavored essence. By the above technical scheme, the inulin, oat meal, soybean dietary fiber, barley leave green juice, konjac powder and psylliumseedhusk which are rich in dietary fiber have the functions of relaxing bowls, regulating constipation, lowering blood cholesterol and triglycerides, controlling body weight and blood sugar and the like, protein needed by the body can be implemented by the addition of the whey protein powder and skinned milk powder which are rich in good protein, and the balanced nutritional diet is achieved in the premise of weight control and reduction of the three highs.
Owner:杭州上医御本医药保健品科技开发有限公司

Novel mycobacterium tuberculosis extracellular nuclease and application thereof

The invention discloses novel mycobacterium tuberculosis extracellular nuclease and application of the novel mycobacterium tuberculosis extracellular nuclease, and proves that Rv (Rotavirus) 0888 is extracellular nuclease of mycobacterium tuberculosis. Based on a sequence analysis, no homology exists between the Rv0888 nuclease and known extracellular nuclease, so that the Rv0888 is proved to be novel extracellular nuclease; activity exerting of Rv0888 protein needs the assistance of divalent ions, and the optimal temperature and the optimal pH (Potential of Hydrogen) of the Rv0888 protein are respective 41 DEG C and 6.5. The invention also provides application of four kinds of traditional Chinese medicine monomers of oleuropein, 6-gingerol, corylifolinin and acteoside for inhibiting the novel mycobacterium tuberculosis extracellular nuclease. Site-directed mutagenesis research shows that a catalytic action of H353 residue, D387 residue and D438 residue on the activity of the Rv0888 is achieved; an in-vivo infection experiment proves that the persistence capacity of recombined mycobacterium smegmatis Rv0888 NS / MS and Rv0888 S / MS in a lung of a mouse is obviously higher than that of pMV262 / MS, obvious pathological change can be generated in the lung of the mouse by the Rv0888 NS / MS and the Rv0888 S / MS, and the Rv0888 is proved to be necessary for mycobacterium infection and pathogenicity.
Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI

Method of efficiently and conveniently purifying proteins

The invention provides a convenient and efficient purification method only requiring centrifugation and cracking steps. Traditional technologies of using column chromatography for purifying recombinant proteins are high in production cost and cumbersome in step; and by adopting non-chromatographic purification methods based on affinity tags, costs can be reduced, the yield of target proteins is increased, but the cost of tag removal is high. In order to solve the problems, the convenient and efficient purification method only requiring the centrifugation and cracking steps is provided. According to the convenient and efficient purification method, an inclusion-body protein CipA and an intein protein (Synechocystis sp.PCC6803 DnaB, Ssp DnaB) are adopted, a self-assembly function of the CipAand a self-cracking function of the DnaB under weak-acid conditions are utilized for achieving protein purification. A target protein can be any protein needing to be expressed and purified, and thepurity of the purified target protein can achieve 95% or above. Besides, according to the convenient and efficient purification method, a connection peptide between the CipA and the DnaB is also optimized, therefore, the cracking efficiency of the DnaB is further improved, and meanwhile influences of the CipA on activities of the target protein are reduced. The convenient and efficient purification method only requiring the centrifugation and cracking steps has the potential of being applied to industrial production of enzymes. Keywords include the protein purification method, the inclusion-body protein CipA, and the self-cracking protein DnaB.
Owner:BEIJING INSTITUTE OF TECHNOLOGYGY

Germination accelerating method for purple carrot seeds

The invention mainly relates to the technical field of planting and discloses a germination accelerating method for purple carrot seeds. The germination accelerating method for the purple carrot seeds comprises the steps of seed dipping, seed fermentation, seed enzymolysis and germination acceleration of the seeds. The germination accelerating method for the purple carrot seeds is simple and specifically comprises the steps that the purple carrot seeds are dipped in an edible alkali solution, so that the seed skin is rapidly softened; seed fermentation is conducted through lactobacillus acidophilus, remaining alkali is removed, the seed skin structures are destroyed, micromolecule nutritional ingredients are generated and promoted to enter the seed skin, and the activity of embryos is improved; after enzymolysis, seed skin external impurities come off, a great number of proteins needed by growth of the seeds is provided at the same time, and the germination rate is improved. In the seed germination accelerating process, through spraying of a germination accelerating agent, nutrients can be provided, insect resisting and sterilizing are achieved, the humidity is kept, the stress resistance of the embryos is improved, growth is accelerated, the germination rate reaches 93%, the germination accelerating time is shortened to 3-4 days, and the planting cost is saved by 15.7%. The components of the germination accelerating agent are simple and wide in source, the utilization ways of grape flowers are increased, and the economic income of each peasant household planting purple carrots is increased by 8.3%.
Owner:杨珊洪

High-protein milk tea powder and preparation method thereof

The invention discloses high-protein milk tea powder and a preparation method thereof. The high-protein milk tea powder comprises vegetable fat powder, protein powder, white granulated sugar, whole milk powder, malt extract, whole egg powder, black tea powder and food additives. The preparation method of the high-protein milk tea powder includes the steps that the home-made protein powder, the vegetable fat powder, the white granulated sugar, the whole milk powder, the malt extract, the whole egg powder, the black tea powder and the food additives are thrown into a mixing pot to be mixed for 20-30 minutes, and the mixed materials are sealed to be packaged in a quantitative mode. By the adoption of the home-made protein powder, the absorption efficiency of protein in the milk tea powder can be effectively improved; meanwhile, due to the fact that the home-made protein powder is combined with the ingredients such as the whole egg powder, nutrients of milk tea are greatly increased, and the acceptable daily intake of the protein needed by a human body is guaranteed.
Owner:魏志华

Health-caring green chicken cakes and preparation method thereof

The invention discloses health-caring green chicken cakes and a preparation method thereof. The health-caring green chicken cakes are prepared from the following raw materials in parts by weight: 30-50 parts of asparagus, 5-10 parts of cucumbers, 10-20 parts of sweet potato powder, 5-10 parts of broad bean powder, 5-10 parts of Chinese yam powder, 5-10 parts of herrings, 2-5 parts of chicken breast meat, 5-10 parts of eggs, 1-3 parts of honey, and 0.5-1 part of edible salt; and the health-caring green chicken cakes are prepared by the following steps of juicing the raw materials, carrying outmixing, carrying out shaping, and carrying out steaming. The health-caring green chicken cakes contain a large amount of amino acids, vitamins and proteins needed by the human body, and have the health-caring values of strengthening the spleen and the stomach and preventing cancer, as well as anti-cancer activity; moreover, the health-caring green chicken cakes are green, clear, refreshing in aroma, palatable, unique in taste, and adaptive to demands of people for green and healthy foods.
Owner:刘宣东

Food-grade fish protein powder and preparation method thereof

The invention discloses a food-grade fish protein powder prepared by deodorizing and degreasing and other processes with low-value fishes as a raw material, and a preparation method thereof. The method mainly comprises that the food-grade fish protein powder is prepared by pretreatment, degreasing, deodorization, cooking, squeezing, drying and crushing of low-value fish flesh. The prepared food-grade fish protein powder has fishy smell, low fat content and high protein content, and can effectively supplement the daily protein needed by a body. Moreover, the preparation method is simple and convenient, the production cost is low, the product quality is high, the economic value of the low-value fishes is effectively increased, and the waste problem of protein resources is solved.
Owner:ZHEJIANG WANLI UNIV

Perch feed formula and preparation method thereof

The invention discloses a perch feed formula and a preparation method thereof, and relates to the technical field of fish farming. In the perch feed formula, imported white fish meal, spray-dried blood globulin powder and antarctic krill powder and other high-quality protein raw materials are added, so that the digestion and absorption rate of perch is high, the growth rate is improved, and the protein needs are met; vitamins, probiotic, and broken yeast powder are comprehensively complemented to enhance the body constitution of perch, improve immunity, promote digestion and absorption and reduce the occurrence of enteritis; the feed formula is treated by an expansion process to effectively improve the absorption and utilization rate of a feed, an expander used is simple in structure and convenient to use, and has high expansion efficiency, and a communicating pipe of the expander can be effectively prevented from being clogged by the feed; the preparation process of the feed formula has the advantages of reducing the damage to the nutrient composition of the feed, ensuring the particle size and mixing uniformity of the feed, increasing the energy level in the feed, and having obvious economic and environmental benefits.
Owner:刘忠宁

Compound feed used for polyculture of bighead silver carps and herrings and preparation method thereof

The invention discloses a compound feed used for polyculture of bighead silver carps and herrings and a preparation method thereof, and relates to the field of fish breeding; and the compound feed used for polyculture of the bighead silver carps and the herrings is composed of a floating feed and a sinking feed. The sinking feed is mainly used for feeding the herrings which live at middle and lower layers of the water; and various animal and plant proteins are used in cooperation so as to provide proteins needed for herring growth. The floating feed is mainly used for feeding the bighead silver carps which live at an upper layer of the water; the floating feed is relatively low in protein content, and is added with various Chinese herbal medicines; and a main purpose of the floating feed is to improve disease resistance of the bighead silver carps. The compound feed adopts different formulas for different fishes so as to avoid nutrient waste; moreover, the compound feed improves survival rate and growth rate of the fishes in polyculture mode so as to increase yield. According to the preparation method of the compound feed used for polyculture of the bighead silver carps and the herrings, differential treatments are carried out during preparation processes according to different feeding habits of the bighead silver carps and the herrings; a fermentation process is added into the preparation processes of the floating feed according to the characteristic of the bighead silver carps, namely fancy on sour rotten foods; and differential feeding of the bighead silver carps and the herrings are ensured.
Owner:黄平县龙井河生态鱼业开发有限责任公司

Method for marking protein

The invention discloses a method for marking protein. The method comprises the following steps: selecting a protein needing to be marked, using a functional molecule with carboxyl or thiocarboxyl as a substrate, using unsaturated sulfonium salt as a carboxyl activator, and carrying out a solid-phase peptide bond generation reaction on the marked protein in an alkaline buffer solution; in a buffer solution, carrying out amine ester exchange reaction activated by unsaturated sulfonium salt and carrying out specific modification reaction on amino groups on the protein; and identifying the specific modification condition by means of western blot or mass spectrum. Functional molecules containing carboxyl or thiocarboxyl are used as substrates, unsaturated sulfonium salt is used as a carboxyl activating agent, and in different types of buffer solutions, different types of various proteins can be marked through amine ester exchange reaction. According to the method, safe and low-toxicity sulfonium salt activated ester is used as a labeling molecule, protein can be labeled efficiently and selectively, and the method can be used for activity-based protein analysis and labeling of antibody molecules in a drug-coupled antibody.
Owner:SHENZHEN BAY LAB PINGSHAN TRANSLATIONAL MEDICINE CENT +2

Health-care protein powder with effect of improving body immunity and preparation method thereof

The invention provides health-care protein powder with the effect of improving the body immunity. The health-care protein powder is prepared by the following components in percentage by weight: 65-85% of soybean isolated protein, 10-15% of whey protein powder, 5-10% of egg yolk immunoglobulin powder, 1-5% of bovine colostrums immunoglubin powder, 2-4% of sesame powder, 0.5-3% of soybean lecithin, 0.5-1% of silicon dioxide, 2-4% of starch, 0.001-0.002% of bifidobacterium, 1-3% of scale extract and 0.001-0.1% of aspartame, and the sum of the percent of the components is 100%. The invention researches and develops a protein type health-care powder with the effect of improving the body immunity; by optimization of the formula and the process, the obtained protein powder is comprehensive in nutrition, not only can supplement the protein needed by a human body and maintain normal immunologic mechanism of the body, but also is low in content of fat in protein type materials, can not cause the cardiovascular disease due to excessive intake of the fat, can be used as a modern health-care food and has wide market prospect.
Owner:GUANGZHOU WANKANG HEALTH CARE PROD CO LTD

Feed for breeding chicks and preparation method of feed

InactiveCN106721451AHelps achieve weight goalsFood processingAnimal feeding stuffEggshellGourd
The invention discloses feed for breeding chicks and a preparation method of the feed. The feed is prepared from raw materials in parts by mass as follows: 25-35 parts of soybean meal, 16-18 parts of shell powder, 8-12 parts of seaweed meal, 8-18 parts of distillers' grains, 13-23 parts of bran powder, 20-35 parts of eggshells, 19-22 parts of corn straw, 30-45 parts of carrots, 25-45 parts of spinach leaves, 25-45 parts of green radish, 8-18 parts of lalang grass rhizome, 30-50 parts of white gourds, 8-18 parts of fructus lycii, 13-15 parts of sweet potato vines and the balance of corn flour. According to the feed for breeding the chicks, the corn and the soybean meal are taken as main materials, a small quantity of antioxidant is added, energy and protein needed for chick growth are provided by the corn and the soybean meal respectively, the 5-week-old chicks are helped to reach the weight standard, and a foundation is laid for follow-up growth.
Owner:唐传猷

Chick feed and preparation method thereof

The invention discloses chick feed and a preparation method thereof, and belongs to the technical field of feed. The feed is prepared from, by weight, 85-95 parts of corn, 30-40 parts of wild buckwheat, 5-10 parts of zeolite powder, 10-20 parts of soybean meal, 5-15 parts of rapeseed meal, 3-8 parts of eggshells, 1-3 parts of pumpkin powder, 5-10 parts of distillers' grains, 1-3 parts of sugarcaneleaves, 2-6 parts of wood vinegar, 0.5-1.5 parts of phytase and 0.5-1.5 parts of diaminocaproic acid. Raw materials of the chick feed are simply and reasonable matched, energy and protein needed by growth of chicken can be completely met, microelements are balanced, and healthy growth of the chicken is facilitated.
Owner:柳州市宏华生物科技有限责任公司

Health drink

The invention relates to a health drink, in particular to a health drink capable of supplying key nutrient elements such as a plurality of microelements and proteins needed by a human body and improving the health conditions of the human body. The health drink mainly aims to solve the problem that the various conventional drinks are all prepared to meet the requirement of human beings on palatability and little attention is paid to the health of consumers. Natural soy bean, which contains rich proteins and microelements and has high output and low price and is well stocked, is taken as the main raw material. The preparation method comprises the following steps of: cleaning and immersing the soy bean, sprouting the soy bean, and crushing the soy bean; adding carrot juice, pumpkin juice, longan juice, medlar juice and shaddock juice; and preparing the mixed raw juice drink according to the modern drink processing technology. The health drink has the advantages of supplying the key nutrient elements such as the plurality of microelements and proteins needed by the human body and improving the health conditions of the human body.
Owner:王吉广

Cultivation method for soybean root nitrogen fixation pleurotus eryngii

The invention discloses a cultivation method for soybean root nitrogen fixation pleurotus eryngii, and belongs to the technical field of fungus cultivation. A pleurotus eryngii culture medium comprises, by mass, 70-80% of soybean straw root smashing fibers, 10-20% of soybean straw smashing fibers, 5-8% of soybean dregs, 1% of lime powder and 1% of gypsum powder. The cultivation method for the soybean root nitrogen fixation pleurotus eryngii has the advantages that soybean roots have the higher nitrogen fixation capacity and can supply sufficient nitrogen sources to pleurotus eryngii strains for growth, soybean straw and the soybean dregs are added to supply lignin and protein needed by the growth of the stains, and the lime powder and the gypsum powder are beneficial for absorption and growth of the pleurotus eryngii; the pleurotus eryngii produced by the culture medium cultivating strains is good in quality, short in cycle, high in yield and good in mouthfeel, the waste soybean roots and straw are used as cultivation raw materials, the waste materials are changed into things of value, therefore, the production cost is saved, economic sustainable development is achieved, and the method has a good development prospect in the field of pleurotus eryngii cultivation.
Owner:CHANGZHOU SIYU ENVIRONMENTAL PROTECTION MATERIAL SCI & TECH

Feed used for juvenile prawns

The invention discloses a feed used for juvenile prawns. The feed comprises, by weight, 25-40 parts of fish meal, 10-20 parts of soybean meal, 10-15 parts of corn flour, 5-10 parts of wheat gluten flour, 5-15 parts of peanut bran, 3-8 parts of soyabean lecithin, 3-5 parts of yeast powder, 3-8 parts of shrimp powder, 0.1-0.5 parts of edible salt and 1-4 parts of a premix. The feed has balanced nutrition, contains various energy and proteins needed by growth of the prawns, and has the advantages of high conversion efficiency, high stability, improvement of the digestion and absorption ability of the prawns, and feed investment reduction, so the production cost is reduced, the disease prevention ability of the prawns is improved, and guaranteeing is provided for producing green and nuisance-free prawns.
Owner:太仓市璜泾新海农机专业合作社

Biscuit with fat-reducing efficacy, and preparation method thereof

The invention relates to a biscuit with fat-reducing efficacy. The formula of the biscuit comprises wheat flour, edible vegetable oil, fresh eggs, oat protein, drinking water, milk powder, green coffee bean extract, edible processing yeast, edible salt, soya lecithin, xylitol, polydextrose, vitamin C, ammonium bicarbonate, calcium dihydrogen phosphate, sodium carbonate, citric acid and tea polyphenol. The biscuit with the fat-reducing efficacy is rich in high-quality protein needed by a human body, carbohydrate with a low glycemic index and water-soluble dietary fibers, has comprehensive and balanced nutrients such as multiple plant active substances facilitating metabolism of superfluous energy inside the body, vitamins, mineral matters and the like, has a low heat content, is rich in Prebiotics optimizing intestinal flora, is free from cane sugar, does not contain trans fatty acids, artificial colors and forbidden medicaments, guarantees that no toxic and side effects exist while reducing heat intake and internal fat accumulation, and enables a user to keep fit while the user enjoys delicious food.
Owner:姜世奎

Maggot larva nutrient solution

The invention discloses a maggot larva nutrient solution. The maggot larva nutrient solution is prepared from 10-20% of brown sugar, 2-3% of calcium nitrate, 3-5% of potassium nitrate, 15-30% of infant milk powder and 42-70% of warm water. The maggot larva nutrient solution contains enough moisture, energy and protein needed by maggots; by adopting the maggot larva nutrient solution, the growth speed of maggot larvae is increased, the spawning quantity of maggot imagoes is increased, and the spawning period is regular.
Owner:XUYI ZHENGTAIYONG BIOLOGICAL ENVIRONMENT TECH CO LTD

Animal feed and preparation method thereof

The invention patent relates to the technical field of feed processing, and discloses an animal feed and a preparation method thereof. The animal feed, according to the mass percentage, comprises thefollowing components: bean cake 20-30%, straw 10-20%, cottonseed hull 5-10%, rapeseed 8-12%, purslane 3-5%, dandelions 4-6%, waste tea 1-4%, and earthworm powder 0.5-1.2%. The animal feed provided bythe invention is simple in preparation, low in cost, rich in nutrients, safe and reliable, has no toxic or side effects; raw materials of natural plants including the purslane, dandelions and waste tea have a balanced arrangement and sufficient nutrients; and the dandelions, purslane and waste tea are also rich in vitamins, mineral amino acids and other substances beneficial to animal growth, andimmunity improvement of the animals; and compared with the traditional animal feed, the feed is added with the high protein earthworm powder to ensure the protein needed for animal growth and is moreconducive to improving the quality of animal meat products.
Owner:孙奕明

Attractive feed for goldfishes and preparation method thereof

The invention provides an attractive feed for goldfishes and a preparation method thereof. The attractive feed for the goldfishes is characterized by being composed of the following ingredients in parts by weight: 10-20 parts of fish meal, 10-20 parts of soybean meal, 3-6 parts of rapeseed meal, 6-12 parts of rice protein powder, 5-10 parts of corn, 10-20 parts of alpha-starch, 5-8 parts of kiwi fruit powder, 3-6 parts of hawthorn fruit powder, 2-5 parts of fish oil, 1-3 parts of calcium dihydrogen phosphate, 2-4 parts of a premix, 10-15 parts of shrimp pulp, 2-8 parts of DMPT, and 2-6 parts of betaine. By using a natural feed raw material, namely the shrimp pulp, as an ingredient of an attractant, the attractive feed is capable of ensuring daily supplement of proteins needed by the goldfishes, as well as attracting the goldfishes to eat by the smell; moreover, the shrimp pulp, the DMPT and the betaine are used together as attractant ingredients, so that synergistic effect can be achieved so as to enhance the attracting effects of the feed.
Owner:TIANJIN CHENHUI FEED

A facile protein purification method for localizing recombinant proteins to the cell surface

This application discloses a simple protein purification method for positioning recombinant proteins on the cell surface, which is characterized in that, by constructing a gene encoding a signal peptide, a chemical cleavage site, and a target protein into an expression vector, the target protein is expressed Locate to the surface of the cells; add the buffer containing the cleavage reagent to the collected cells to resuspend, and the supernatant after centrifugation is the target protein solution. The simple protein purification method of the present application utilizes the localization function of the signal peptide, so that the recombinant protein is secreted and localized to the surface of the cell after being expressed in the cell. And taking advantage of the chemical cleavage site in the middle of the recombinant protein, you only need to add a cleavage reagent to the buffer used to resuspend the cells, and centrifuge to obtain a high-purity target protein very easily. It solves the cumbersome steps of lysing cells and using chromatographic columns of various properties to obtain the target protein in the prior art for protein purification, and the operation is simple, which greatly saves time and cost.
Owner:BIOINTRON BIOLOGICAL INC

Preparation method of recombinant protein

InactiveCN102719476AReached production volumeGood natural activityDepsipeptidesVector-based foreign material introductionTransgeneBioreactor
The invention discloses a preparation method of recombinant protein. In existing methods, some methods are high in cost and low in output, some methods are both high in technical requirements and cost, and preparing new protein needs long period, and is high in risk and poor in flexibility. The invention is characterized by cloning a recombinant protein gene into a eukaryotic expression vector and transfecting the vector into a mice ascites cell line, building a stabilized transfection transgene cell line through screening in vitro and cloning, and injecting the cell line into a mice abdominal cavity; and using the mice abdominal cavity as a transgene bioreactor for the transgene cell line to express the recombinant protein, extracting ascites after the ascites is formed and extracting and purifying the recombinant protein from the ascites. According to the method, the preparation of the recombinant protein is not only simple, highly efficient, quick and well applicable, but also low in cost, high in yield and good in natural activity of the recombinant protein.
Owner:ZHEJIANG CHINESE MEDICAL UNIVERSITY

Natural protein substance compound lotion

The invention discloses a natural protein substance compound lotion. The natural protein substance compound lotion is prepared from chlorhexidine gluconate and peptidoglycan; generally, the natural protein substance compound lotion is added into water so as to be prepared into the lotion, the concentration of the chlorhexidine gluconate in the lotion is 0.1 to 1 percent g / ml, and the concentration of the peptidoglycan in the lotion is 0.05 to 1 percent g / ml. The natural protein substance compound lotion disclosed by the invention is used for restraining bacillus coli, staphylococcus aureus and tritirachium album. Generation of obvious bioactivity between the chlorhexidine gluconate and the peptidoglycan is promoted through combination of the chlorhexidine gluconate and the peptidoglycan, the excellent treatment effect is generated, peripheral blood cells can be increased while the bacteriostatic activity of the chlorhexidine gluconate is expressed, various protein needed for skin repairing can be compounded, macrophage and lymphocyte can be activated.
Owner:TIANJIN SIBANGSHI BIOTECH

Jumble bead meat strips and production method thereof

The invention provides jumble bead meat strips. The jumble bead meat strips comprise main materials, auxiliary materials and food additives, wherein the main materials comprise 45%-50% of lean meat, 15%-20% of marbled meat, 10%-15% of chicken meat and 13%-16% of jumble beads; the auxiliary materials comprise 5%-8% of white granulated sugar, 2%-2.5% of monosodium glutamate, 1%-1.2% of table salt and 0.5%-0.8% of sorbitol; the food additives comprise 0.15%-0.2% of composite phosphate, 0.02%-0.05% of d-sodium erythorbate, 0.03%-0.04% of ethyl maltol, 0.006%-0.008% of sodium nitrite and 0.2%-0.4% of spices; and all the percentages are weight percentages. The jumble bead meat strips have abundant nutrition and excellent taste; the lean meat, the marbled meat, the chicken meat and the jumble beads contain protein needed by a human body, and the four materials are combined to meet the intake amount of the protein of the human body; the jumble beads also contain other substances needed by the human body, such as sugar, vitamin B group, potassium, ferrum and phosphorus, so that the activation on heart blood vessels can be promoted. The marbled meat and the chicken meat can be used for improving the mouth feel of the jumble bead meat strips; and after being cooked, the jumble beads can dissipate soft and sweet aroma, so that the product is relatively easily favored by a lot of people.
Owner:JINJIANG QINGWEIYUAN FOOD CO LTD
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