57 results about "Chemical Cleavage" patented technology

Methods and compositions are provided which inhibit the apoptotic activity associated with oxidative stress in many disease states. According to the invention, inhibition of chemical cleavage of PKCδ by caspase-3 results in reduction of apoptosis. Novel peptide inhibitors with the amino acid motif Asp Ile Pro Asp (SEQ ID NO:5) are also disclosed. The peptides are useful as inhibitors of PKCδ-mediated apoptosis and oxidative stress, and other diseases regulated by a catalytically active PKCδ.

Disclosed is a highly sensitive photothermographic material containing on a support a silver salt of an organic acid, a photosensitive silver halide, a reducing agent, a binder and, for example, a compound of which one-electron oxidized derivative produced by one electron oxidation of the compound is capable of releasing two or more electrons with a bond cleavage.

The present invention relates to methods of employing a metal-organic framework (MOF) as a catalyst for cleaving chemical bonds. In particular instances, the MOF results in selective bond cleavage that results in hydrogenolyzis. Furthermore, the MOF catalyst can be reused in multiple cycles. Such MOF-based catalysts can be useful, e.g., to convert biomass components.

Disclosed is a highly sensitive photothermographic material containing on a support a silver salt of an organic acid, a photosensitive silver halide, a reducing agent, a binder and, for example, a compound of which one-electron oxidized derivative produced by one electron oxidation of the compound is capable of releasing two or more electrons with a bond cleavage.

A silver halide photosensitive material containing a compound selected from Types 1-4 below which is capable of undergoing a one-electron oxidation to form a one-electron oxidation product (OEOP), and a reducing compound (Type 1) the OEOP is capable of releasing further two or more electrons accompanying a subsequent bond cleavage reaction, (Type 2) the OEOP is capable of releasing further one electron accompanying a subsequent bond cleavage reaction, and the compound having, in its molecule, two or more groups adsorptive to silver halide, (Type 3) the OEOP is capable of releasing further one or more electrons after going through a subsequent bond forming process, and (Type 4) the OEOP is capable of releasing further one or more electrons after going through a subsequent intramolecular ring cleavage reaction.

This invention relates to a group of novel sapogenins, their use in anti-cancer applications, and to a process for their production. More particularly, this invention pertains to a novel group of dammarane sapogenins, PAM-120, PBM-110 and PBM-100 (the dammarance sapogenine structure is specifically clean of any sugar moieties (glycons) at any position and hydroxyl at C-20) and PAN-20 and PAN-30 (the dammarance sapogenin structure has sugar moieties but is free of hydroxyl at C-20), obtained by chemical cleavage of dammarane saponins. The invention also includes a novel application of the said sapogenins for anti-cancer treatment by using them separately or together, and / or jointly with other drugs, as well as to the process of producing these novel sapogenins. Said novel dammarane sapogenins show surprising anti-cancer effect when applied, particularly against multi-drug resistant cancers.

The invention includes a method of sample profiling for identification. The method includes the steps of performing less than four nucleotide-specific chemical cleavage reactions to obtain nucleotide sequence fragments, performing size separation on the fragments, detecting the fragments' separation, generating a profile based on the detection, and comparing the profile to a data base to identify the sample.

This invention provides a photographic element comprising at least one silver halide emulsion layer in which the silver halide is sensitized with a compound of the formula (a):wherein DELTA is protective group that is eliminated during development of the photographic element, t is a timing group, m is an integer from 0 to 3, and XY' is a fragmentable electron donor moiety in which X is an electron donor group and Y' is a leaving proton H or a leaving group Y, with the proviso that if Y' is a proton, a base, beta-, is present in the emulsion or is covalently linked directly or indirectly to X, and wherein:1) X-Y' has an oxidation potential between 0 and about 1.4 V;2) the oxidized form of X-Y' undergoes a bond cleavage reaction to give the radical X. and the leaving fragment Y', and3) the radical X. has an oxidation potential <=-0.7V.

Disclosed is a silver halide photographic light-sensitive material comprising at least one silver halide emulsion layer on a support, which contains a compound of which one-electron oxidized derivative produced by one electron oxidation of the compound is capable of releasing two or more electrons with a bond cleavage and has a characteristic curve drawn in orthogonal coordinates of logarithm of light exposure (x-axis) and optical density (y-axis) using equal unit lengths for the both axes, on which gamma is 5.0 or more for the optical density range of 0.3-3.0. This silver halide photographic light-sensitive material shows high contrast and high sensitivity.

The present invention discloses an improved process for the production of desired recombinant peptides from bacterial cells by using G-CSF as a novel fusion partner for their high level expression in these cells. The invention further provides an expression system comprising the fusion protein wherein the G-CSF is operatively linked to the peptide of interest via an enzymatic or chemical cleavage site which can be used to separate the fusion partner from the said peptide.

The invention discloses a method for extracting nucleic acid from activated carbon biological membranes in drinking water treatment and relates to the technical field of microbiology of biological activated carbon process units in environmental engineering drinking water deep treatment. The method includes: firstly, subjecting activated carbon particles to dehumification by the aid of dehumification buffer solution; and then performing water bath and ultrasonic elution. The method specifically includes: subjecting activated carbon to dehumification by the aid of the dehumification buffer solution obtained by mixing NaOH solution or NaOH with polyvinylpyrrolidone, and extracting the microbial nucleic acid on the biological activated carbon after ultrasonic elution and chemical cleavage. The total DNA (deoxyribonucleic acid) length is larger than 20 Kb so as to completely meet the requirements of PCR (polymerase chain reaction) analysis. According to the method, the problem of difficulty in elution resulted from low biomass of the biological activated carbon and close combination of the biological activated carbon with carrier particles is solved, and adverse effect of humic acid on microbial nucleic acid extraction is weakened, so that microbial cells are sufficient in cleavage. The method is simple and convenient to operate and high in DNA output and purity.

The invention relates to a preparation method of pyrimidine dione compounds. According to the preparation method, the pyrimidine dione compounds are efficiently synthesized in a solvent by taking cyclic oxime ester compounds and isocyan compounds as reaction raw materials under the protection of inert gases and the catalytic action of univalent silver compounds. The preparation method provided by the invention has the advantages of low price of the raw materials, easy obtaining of the raw materials, short steps, high atom economy (100%) and mild reaction conditions, and can realize large-scale industrial application; by using cyclic oxime ester N-O bond cleavage, the pyrimidine dione compounds are efficiently constructed in one step. The breakthrough progress in chemical synthesis of a system is realized, and the deep expansion of pharmaceutical chemistry studies related to the system is promoted.

A method for increasing peptide fragmentation by labeling the peptide at the C-terminal end with a guanidinium group or other basic functional group and distinguishing isobaric amino acids and amino acid combinations of asparagine and glycine-glycine; glutamine and glycine-alanine; and / or glutamine and alanine-glycine, during polypeptide sequencing. The method involves: obtaining a peptide of interest and / or digesting a polypeptide of interest with a protease, such as pepsin, chymotrypsin or trypsin, or by chemical cleavage to produce shorter peptides; reacting the obtained and / or generated peptides with a coupling reagent to derivatize the free C-terminal carboxylic acid function of the peptides, thus adding a basic functional group rendering C-terminal peptide fragment ions detectable by mass spectrometry; selecting a charge state of 2+ or more, and fragmenting the derivatized peptides in a mass spectrometer under conditions effective to generate at least w ions; and detecting the w ions by mass spectrometry, and identifying derivatized peptides which incorporate the additional mass of the basic functional group.

The invention discloses an ether bond breaking method of phenyl alkyl ether. The ether bond breaking method comprises the following step: in an organic solvent, in the presence of aluminum triiodide and an inorganic acid scavenger, performing an ether bond breaking reaction on the phenyl alkyl ether from -20 DEG C to the refluxing temperature to produce phenol and derivatives thereof. The ether bond breaking method is mild in condition, easy to operate and high in yield, and is suitable for a wide range of phenyl alkyl ether.

A silver halide color photosensitive material comprises a blue-sensitive layer, a green-sensitive layer, a red-sensitive layer and a non-light-sensitive layer on a support. The silver halide color photosensitive material contains a compound selected from the following type 1 and type 2 compounds, and wherein the blue-sensitive layer meets the relationship of the following formula (I):SB(370 nm) / SB(420 nm)<0.7  (I)wherein SB(λ) represents a spectral sensitivity at a wavelength of λ,(type 1)a compound capable of undergoing a one-electron oxidation to thereby form a one-electron oxidation product thereof, wherein the one-electron oxidation product is capable of releasing further one or more electrons accompanying a subsequent bond cleavage reaction, and(type 2)a compound capable of undergoing a one-electron oxidation to thereby form a one-electron oxidation product thereof, wherein the one-electron oxidation product is capable of releasing further one or more electrons accompanying a subsequent bond-forming reaction.