58 results about "Infection experiment" patented technology

The invention provides a method for producing porcine circovirus type II capsid protein by utilizing a silkworm bioreactor, and belongs to the field of biotechnology. In the method, by taking a bombyx nuclear polyhedrosis virus as a vector, porcine circovirus type II capsid protein genes are integrated into a polyhedrosis promoter of the bombyx nuclear polyhedrosis virus so as to express target protein by a mode of the homologous recombination of a homologous arm of the nuclear polyhedrosis virus in a transfer vector and bombyx nuclear polyhedrosis virus (BmNPV) genes; a recombinant bombyx nuclear polyhedrosis virus containing target protein genes is obtained by a plaque sieving method, and the target protein is expressed in large scale by using a bombyx bioreactor so as to prepare a subunit vaccine containing recombinant porcine circovirus type II capsid protein; and piglet infection experiments verify that the subunit vaccine has the excellent immune protective effect. The method has the characteristics of high expression efficiency, good activity of the target protein, low production cost and the like, and is suitable for large-scale production.

The invention provides a bacterial infection experiment device for cell culture dishes. The device comprises an airtight bacterial infection cabin mounted on a movable support leg frame, wherein the airtight bacterial infection cabin is provided with a sealing cabin door and a cabin door handle lock on the front surface, the sealing cabin door can be opened / closed and locked, a plurality of in-cabin trays are arranged on the cabin rear wall in the bacterial infection cabin and can accommodate the cell culture dishes to be exposed in the infection experiment environment, an atomization diffuser is connected to the upper part of the bacterial infection cabin, atomizes and diffuses a solution containing bacteria and conveys the solution to the cell culture dishes for an exposure infection experiment, the lower end of the bacterial infection cabin is connected to a waste gas treater in a sealed manner, the waste gas treater disinfects, sterilizes and efficiently filters waste gas from the bacterial infection cabin, safe emission of exhaust is guaranteed, and an ultraviolet disinfection lamp in the bacterial infection cabin is used for sterilization and disinfection after the experiment is completed. The bacterial infection experiment device has the benefits as follows: a safe and convenient bacterial infection experiment device having a simple structure and used for the cell culture dishes is developed, and the difficulty of automatic operation and sealed isolation in bacterial infection research work of the cell culture dishes is solved.

The invention provides a mouse fixing device for schistosome infection experiments and an experiment method. The mouse fixing device for schistosome infection experiments comprises a base plate; the upper end surface of the base plate is provided with a working area used for placing a mouse; each of the two sides of the working area is provided with a plurality of holes, in which two holes are locating holes; elastic pull bands penetrate through the locating holes; the upper ends of the elastic pull bands are fixed to limbs of the mouse and the other ends penetrate through the locating holes and are fixed to the base plate via limiting members to press the limbs of the mouse to the base plate tightly; the base plate is also provided with a clamp used for fixing and pressing the neck of the mouse to the base plate. The mouse fixing device for schistosome infection experiments is simple in structure, can fix the four limbs and the neck of the mice of different sizes in the experimental process, can prevent the mice from get injured and prevent the mice from biting workers in the experimental process, and enables the workers to record the data of the mice in the schistosome infection experiment process timely and perform timed statistics on the data for progress of later experiments.

The invention discloses an application of secoisolariciresinol diglucoside in preparation of a drug with resistance to eimeria tenella. Powder is prepared from the secoisolariciresinol diglucoside and acceptable auxiliary materials, is added to feed and is mixed uniformly, and chickens are fed according to the daily amount of feed; or water-soluble secoisolariciresinol diglucoside powder is prepared from the secoisolariciresinol diglucoside and dissolved in water, and the chickens are fed according to the daily drinking amount. Animal infection experiments prove that the ACI (anti-coccidial index) of the secoisolariciresinol diglucoside can be up to 165 or higher, and the secoisolariciresinol diglucoside has a better eimeria tenella resistant effect. Compared with a conventional anti-coccidial drug, namely, sulfachloropyrazin, the secoisolariciresinol diglucoside has a remarkable eimeria tenella resistant effect. According to the application of the secoisolariciresinol diglucoside, the problem of severe drug tolerance produced through adoption of the anti-coccidial drug in the current breeding industry can be effectively solved, the research and development speed of a new drug can be increased on the basis, and great benefits are provided for green and healthy development of the breeding industry.

The invention relates to a cream medicine composition for treating and preventing foot superficial fungal infection and a preparation method thereof. The cream is prepared from the following components: melaleucaleucadendron essential oil, L-menthyl lactate, camphor, menthol, laurocapram and cream substrates, wherein the cream substrates comprise vaseline, octadecanol, atoleine, dehydrated sorbitan monostearate, GMS (Glycerin Monostrearate), dimethyl silicone oil, fatty alcohol polyoxyethylene ether, sodium lauryl sulfate, propylene glycol, emulsified silicone oil and the like. The cream has a scientific formula and has the effects of preventing the foot superficial fungal infection and relieving foot skin itch. Proved by experiments, the melaleucaleucadendron essential oil in the cream can highly penetrate into the skin surface, has a remarkable effect of preventing the pathogenic foot superficial fungal infection and is an effective preparation for preventing the foot superficial fungal infection. Proved by a mouse infection experiment, the effective ratio of the cream disclosed by the invention to prevent the foot superficial fungal infection of a mouse is up to 90.5%. The preparation process flow of the preparation is reasonable, chemical structures of various raw materials and a penetration auxiliary agent are not broken, and the operation is easy to carry out.

The invention discloses novel mycobacterium tuberculosis extracellular nuclease and application of the novel mycobacterium tuberculosis extracellular nuclease, and proves that Rv (Rotavirus) 0888 is extracellular nuclease of mycobacterium tuberculosis. Based on a sequence analysis, no homology exists between the Rv0888 nuclease and known extracellular nuclease, so that the Rv0888 is proved to be novel extracellular nuclease; activity exerting of Rv0888 protein needs the assistance of divalent ions, and the optimal temperature and the optimal pH (Potential of Hydrogen) of the Rv0888 protein are respective 41 DEG C and 6.5. The invention also provides application of four kinds of traditional Chinese medicine monomers of oleuropein, 6-gingerol, corylifolinin and acteoside for inhibiting the novel mycobacterium tuberculosis extracellular nuclease. Site-directed mutagenesis research shows that a catalytic action of H353 residue, D387 residue and D438 residue on the activity of the Rv0888 is achieved; an in-vivo infection experiment proves that the persistence capacity of recombined mycobacterium smegmatis Rv0888 NS / MS and Rv0888 S / MS in a lung of a mouse is obviously higher than that of pMV262 / MS, obvious pathological change can be generated in the lung of the mouse by the Rv0888 NS / MS and the Rv0888 S / MS, and the Rv0888 is proved to be necessary for mycobacterium infection and pathogenicity.

The invention relates to a silver crucian carp back fin cell line. The silver crucian carp back fin cell line is obtained through primary culture and subculture conducted on the back fin of a silver crucian carp, and a used cell culture fluid is prepared by using an M199 culture medium. The invention further provides an establishing method and application of the silver crucian carp back fin cell line. The silver crucian carp back fin cell line has the advantages that the established silver crucian carp back fin cell line can be subjected to continuous passage, and a large amount of silver crucian carp back fin cell lines can be provided. The silver crucian carp back fin cell line can be directly applied to research on silver crucian carp immunity related function genes and can be applied to a silver crucian carp virus infection experiment, a large amount of manpower and material resources are saved, and effect is better. The silver crucian carp back fin cell line can be subjected to multigelation and can be applied to important research in cell biology, virology, molecular biology, genetics, immunology and the like.

The invention discloses application of paeonol in preparing a medicine for resisting eimeria tenella. Paeonol and acceptable auxiliaries are prepared to form powder; the powder is added into a feed, is uniformly mixed, and is used for feeding chicken according to a daily feeding amount; or paeonol water-soluble powder is prepared from paeonol, can be dissolved in water, and can be used for feeding chicken according to a daily feeding amount. Animal infection experiments prove that the anticoccidial index (ACI) of paeonol can be over 180, and paeonol has a good effect of resisting eimeria tenella.

The invention discloses a biological safety negative pressure isolator for poultry. The biological safety negative pressure isolator comprises an operation box body, wherein an equipment layer and a bracket are respectively arranged on the upper and lower sides of the operation box body; an air supply and filter device and a first-stage air exhaust and filter device are respectively mounted at the left and right ends of the bottom of the equipment layer; the top of the operation box body communicates with the first-stage air exhaust and filter device and the air supply and filter device; a second-stage air exhaust and filter device communicating with the first-stage air exhaust and filter device is mounted at the bottom of the bracket; the left end of the second-stage air exhaust and filter device communicates with a gas disinfectant discharging outlet and a fan; the operation box body is provided with a pair of operation gloves; an equipment door is mounted on the left side wall of the operation box body and a first air tight door is mounted on the right side wall of the operation box body; the first air tight door communicates with a detachable transmission device with a second air tight door; and a liquid trough transmission device is arranged at the left end of the bracket. The biological safety negative pressure isolator for the poultry can enable experiment personnel to carry out various isolation operations such as poultry infection experiment, breeding and pestilence inspection and quarantine, is easy and convenient to operate, is high in air tightness, and can transmit an experimental object conveniently and safely.

The invention discloses an application of rhodosin in preparing anti-eimeria tenella drug. The application comprises the following steps: preparing powder from the rhodosin and acceptable auxiliary materials, adding the powder into a feed, uniformly mixing the mixture, and feeding chicken according to a daily feed amount, wherein the adding amount of rhodosin is 5g in every 100kg of feed; or preparing rhodosin into rhodosin water soluble powder, dissolving the powder in water, feeding chicken according to daily water consumption, wherein the adding amount of rhodosin is 2.5g dissolved in every 100kg of water. Animal infection experiments verify that the anti-coccidia index (ACI) of the rhodosin can reach 175 above, and the rhodosin has relatively good anti-eimeria tenella action and is expected to be applied to preparing the anti-eimeria tenella drug.

The invention discloses an II-type grass carp reovirus cold-adapted strain GCRV-GD108ca and application thereof, and belongs to the technical field of prevention and treatment of viral hemorrhagic diseases of fishes. The cold-adapted attenuated vaccine strain GCRV-GD108ca disclosed by the invention has a good protection effect on viral hemorrhagic disease of grass carp, and can effectively resist attack of homologous or heterologous type II grass carp reovirus. A safety experiment shows that the GCRV-GD108ca strain is safe to a target animal, is injected into grass carps in an over-dose manner, and does not cause morbidity and death phenomena of experimental fishes. Continuous passage is carried out on cells and fish bodies and susceptible animal toxicity attacking experiments are carried out, wherein the results show that the attenuated strain is stable in toxicity and free of toxicity enhancement; besides, symbiosis infection experiments show that the cold-adapted strain is safe to different grass carp polyculture varieties such as silver carp and crucian carp, does not generate horizontal propagation phenomenon and has no side effect. The invention provides a technical scheme for preventing and treating viral hemorrhagic disease of grass carp, and has important economic value and social significance.

The invention discloses a cell culture dish bacterium infection experiment device. The cell culture dish bacterium infection experiment device comprises a liquid inlet module, a gas inlet module, a cell culture module, a gas outlet module, a sterilizing module and a control module, wherein the gas inlet module is used for ventilating external gas; the liquid inlet module is used for transporting bacterium culture solution to the cell culture module for performing cell culture; the gas outlet module is used for exhausting waste gas; the sterilizing module is used for sterilizing the cell culture module; and the control module is used for controlling the liquid inlet module, the gas inlet module, the cell culture module, the gas outlet module and the sterilizing module; and the cell culturemodule comprises a bin body which can be subjected to sealing control, wherein the bin body is divided into a first bin chamber, a second bin chamber and a third bin chamber; a disk culture frame is arranged in the first bin chamber; a linear culture frame is arranged in the second bin chamber; and a blank group frame is arranged in the third bin chamber. According to the cell culture dish bacterium infection experiment device disclosed by the invention, through a plurality of control groups, cell culture can be intuitively and full-automatically performed, so that the convenience and the accuracy of the experiment are improved.

The invention discloses an application of hexachlorophene in preparation of a drug resistant to Eimeria tenella. The hexachlorophene and pharmaceutically acceptable excipients are evenly mixed and added in water or feed. The animal infection experiments confirm that the anticoccidial index (ACI) of the hexachlorophene reaches over 168.8, and the hexachlorophene has better effect in resistance to the Eimeria tenella.

The invention discloses a novel use of an aspartic endopeptidase gene. The use is an application of the aspartic endopeptidase gene as a drug target in preventing and / or controlling tobacco brown spot. The nucleotide sequence of the spartic endopeptidase gene is shown in SEQ ID NO:1. According to the invention, a wild-type strain of A. longipes and a Pep1 gene knockout strain are taken as researchobjects for carrying out a tobacco leaf infection experiment in vitro, and the result shows that the pathogenicity of the Pep1 gene knockout strain to tobacco leaves is obviously reduced, so that theaspartic endopeptidase gene is a key pathogenic factor in the A. longipes, and can be used as a drug target for screening drugs for preventing and / or controlling the tobacco brown spot. An aspartic endopeptidase inhibitor can be used to prepare the drugs for preventing and controlling the tobacco brown spot.

The invention discloses the application of baicalin in preparation of medicine for resisting eimeria tenella. A powder is prepared from baicalin and acceptable ingredients, and is added into fodder tobe blended for feeding chicken according to everyday fodder quantity; or baicalin is prepared into a baicalin water soluble powder to be dissolved into water to feed chicken according to everyday fodder quantity. Animal infection experiments indicate that the anticoccidial index (ACI) of baicalin can reach more than 175, and the baicalin has preferable resistance to eimeria tenella, therefore, anovel application of baicalin medicine is provided.

The invention discloses an application of alantolactone in preparation of medicines for resisting cryptosporidium parvum. The application method comprises the following steps: preparing powder from alantolactone and acceptable auxiliary materials; adding to a feed, mixing evenly, and feeding mouse according to the daily feed amount; or preparing an alantolactone water-soluble power from the alantolactone, dissolving into water, and feeding the mouse according to the daily water consumption. An animal infection experiment proves that the oocysts after cryptosporidium parvum injection can be reduced by 75% or above by the alantolactone; and the alantolactone has a relatively good effect of resisting cryptosporidium parvum.

The invention provides a pathogenic bacterium of laver filament albinism. The preservation number of the pathogenic bacterium is CGMCC No.21174. The strain provided by the invention is applied to screening of an albinism-resistant laver strain. A free protonema infection experiment proves that the laver protonema albinism pathogenic bacterium, namely, the rokella GN-R-1 strain obtained by screening can cause albinism of healthy laver protonema and is a pathogenic bacterium of albinism in the laver seedling stage; the separation and discovery of the strain are beneficial to porphyra albinism identification and disease prevention and control, and the strain can be used for screening porphyra strains with albinism resistance.

The invention discloses chrysin and application of medicinal derivatives of the chrysin in preparing cryptosporidium parvum resisting medicine or feed additives. The chrysin and the medicinal derivatives of the chrysin have excellent cryptosporidium parvum resisting activity, an animal infection experiment proves that the ovulation quantity of cryptosporidium parvum can be reduced by more than 70% by adding the chrysin, and the chrysin has an excellent cryptosporidium parvum resisting effect and is expected to be developed into the cryptosporidium parvum resisting medicine or feed additives. In addition, the chrysin is easy to prepare and low in extraction cost and can be easily developed into the low-cost cryptosporidium parvum resisting medicine or feed additives.

The invention discloses a mild bacteriophage VneM1 for regulating and controlling coral flora and application of the mild bacteriophage VneM1. The preservation number of the bacteriophage is Vibrio neocaledonicus bacteroiphage VneM1 is CGMCC NO.61215-B1. The invention also discloses a preparation method of the bacteriophage. According to the invention, the new bacteriophage-Vibrio neocaledonicus bacteriophage VneM1 is separated from a coral symbiotic bacterium Vibrio neocaledonicus SCSIO 43009. The bacteriophage VneM1 has a cracking effect on coral symbiotic vibrio, and regulates and controlsthe composition of coral symbiotic microorganisms. Hydranth infection experiments prove that the bacteriophage VneM1 can delay coral whitening caused by temperature rise. The bacteriophage VneM1 is amild bacteriophage, and under normal conditions, the bacteriophage VneM1 is integrated on an original lysozyme SCSIO 43009 genome and exists in a form of a prophage. The bacteriophage can be producedby spontaneous lysogen-lysis conversion, and the lysogen-lysis conversion can be promoted by environmental factors such as SOS stress reaction.

The invention discloses a compound fluconazole injection and a preparation method thereof, wherein the compound fluconazole injection comprises fluconazole, ivermectin and an organic solvent. According to the present invention, the preparation process is simple, and the proper ratio of the injection is determined through a large number of experiments, wherein per 1000 ml of the injection contains 5-100 g of fluconazole and 5-30 g of ivermectin; and the pet inside and outside parasite and fungal infection experiment results prove that the prepared injection has the stable quality, the treatment effect is significant, the cure rate can achieve 100%, and the defect of the use of the single reagent is overcome.

The invention provides an application of genkwanin in preparation of a drug for resisting Listeria monocytogenes infection. Rabbit erythrocyte hemolysis test, J774 cell protection effect test and mouse infection experiment therapeutics test prove that the genkwanin can effectively inhibit the pore-forming activity of Listeria monocytogenes hemolysin at an extremely low concentration, can inhibit the cytotoxic effect of Listeria monocytogenes on J774 cell mediation, and has a good treatment effect on mouse infection caused by the Listeria monocytogenes. According to the invention, Listeria monocytogenes infection is treated by the genkwanin. Compared with the traditional antibiotic treatment, the genkwanin is not easy to induce the generation of drug resistance, has lower selection pressureon bacteria, has no obvious toxic effect in the test, and has higher cure rate. Therefore, the genkwanin can be used for new drug development and has important significance for drug target confirmation.

The invention discloses application of triclosan to preparation of medicaments for resisting Eimeria tenella. The triclosan and pharmaceutically acceptable accessories are uniformly mixed and the mixture is added into water or feeds. Animal infection experiments prove that the anticoccidial index (ACI) of the triclosan reaches over 177.9, and the triclosan has better effect of resisting Eimeria tenella.

The invention discloses a monoclonal antibody having PEDV neutralizing activity. The monoclonal antibody having the PEDV neutralizing activity and provided by the invention is secreted by a monoclonalantibody hybridoma cell strain SWS-2 having the PEDV neutralizing activity. The monoclonal antibody hybridoma cell strain SWS-2 having the PEDV neutralizing activity has the registration number of CGMCC No.18312 in China General Microbiological Culture Collection Center. The monoclonal antibody having PEDV neutralizing activity is prepared, so that newborn piglets orally take the antibody, the infection probability of the piglets can be effectively reduced, and the protection rate reaches 100% in a cohabitant infection experiment. Results show that the monoclonal antibody having PEDV neutralizing activity can effectively increase the antibody content in the newborn piglets and reduce the risk of illness; the method is simple to prepare and convenient to operate and has a great applicationprospect.

The invention discloses application of Irosustat in preparing drugs resistant to eimeria tenella. Irosustat and acceptable auxiliary materials are prepared into powder to be added into feeds with even mixing, and chicken are fed according to daily feed quantity; or the Irosustat is prepared into Irosustat water soluble powder to be dissolved into water, and the chicken are fed according to daily feed quantity. Animal infection experiments prove that the anticoccidialindex (ACI) of the Irosustat can reach to 185. Therefore, the Irosustat has a good function of resisting the eimeria tenella.

The invention provides a bacterial infection experiment device for cell culture dishes. The device comprises an airtight bacterial infection cabin mounted on a movable support leg frame, wherein the airtight bacterial infection cabin is provided with a sealing cabin door and a cabin door handle lock on the front surface, the sealing cabin door can be opened / closed and locked, a plurality of in-cabin trays are arranged on the cabin rear wall in the bacterial infection cabin and can accommodate the cell culture dishes to be exposed in the infection experiment environment, an atomization diffuser is connected to the upper part of the bacterial infection cabin, atomizes and diffuses a solution containing bacteria and conveys the solution to the cell culture dishes for an exposure infection experiment, the lower end of the bacterial infection cabin is connected to a waste gas treater in a sealed manner, the waste gas treater disinfects, sterilizes and efficiently filters waste gas from the bacterial infection cabin, safe emission of exhaust is guaranteed, and an ultraviolet disinfection lamp in the bacterial infection cabin is used for sterilization and disinfection after the experiment is completed. The bacterial infection experiment device has the benefits as follows: a safe and convenient bacterial infection experiment device having a simple structure and used for the cell culture dishes is developed, and the difficulty of automatic operation and sealed isolation in bacterial infection research work of the cell culture dishes is solved.

The invention provides a method for producing porcine circovirus type II capsid protein by utilizing a silkworm bioreactor, and belongs to the field of biotechnology. In the method, by taking a bombyx nuclear polyhedrosis virus as a vector, porcine circovirus type II capsid protein genes are integrated into a polyhedrosis promoter of the bombyx nuclear polyhedrosis virus so as to express target protein by a mode of the homologous recombination of a homologous arm of the nuclear polyhedrosis virus in a transfer vector and bombyx nuclear polyhedrosis virus (BmNPV) genes; a recombinant bombyx nuclear polyhedrosis virus containing target protein genes is obtained by a plaque sieving method, and the target protein is expressed in large scale by using a bombyx bioreactor so as to prepare a subunit vaccine containing recombinant porcine circovirus type II capsid protein; and piglet infection experiments verify that the subunit vaccine has the excellent immune protective effect. The method has the characteristics of high expression efficiency, good activity of the target protein, low production cost and the like, and is suitable for large-scale production.

The invention discloses application of paeonol in preparing a medicine for resisting eimeria tenella. Paeonol and acceptable auxiliaries are prepared to form powder; the powder is added into a feed, is uniformly mixed, and is used for feeding chicken according to a daily feeding amount; or paeonol water-soluble powder is prepared from paeonol, can be dissolved in water, and can be used for feeding chicken according to a daily feeding amount. Animal infection experiments prove that the anticoccidial index (ACI) of paeonol can be over 180, and paeonol has a good effect of resisting eimeria tenella.