60 results about "Anticoccidial Agents" patented technology

There is provided an animal feed, an anticoccidial agent, and an anticlostridial agent having less harm and an excellent prophylactic and / or therapeutic effect for coccidiosis and clostridial disease. Also provided is a prophylactic and / or therapeutic feed for coccidiosis containing pinene, thymol, eugenol, and limonene; an anticoccidial agent containing pinene, thymol, eugenol, and limonene as active ingredients; a clostridial disease prophylactic and / or therapeutic feed containing pinene, thymol, eugenol, and limonene; and an anticlostridial agent containing pinene, thymol, eugenol, and limonene as active ingredients.

The invention relates to a kind of slow-releasing parenteral injection and the preparative way of it. The product can prevent coccidiosis of chickens and rabbits. The ingredients of the parenteral injection include: 3-6 shares (by weight) abrotani herba and kuhseng, 2-5 shares (by weight) wood anemone, agrimony and bupleurum root, 2-4 shares (by weight) beta-cyclodextrin. The preparative way of the product goes as follows: lixiviate the materials with 95% ethyl alocohol twice to get A1 solution; extract the residue with water twice to get A2 solution; remove the tannin in A1 and A2 by heating alternating with cooling; envelop the material with beta-cyclodextrin to get the product.

The invention relates to a decoquinate nanoemulsion coccidium-resisting drug. The particle size of the decoquinate nanoemulsion coccidium-resisting drug is 1-100nm, the decoquinate nanoemulsion coccidium-resisting drug consists of, by mass percentage, decoquinate 0.1-6.0%, surface active agent 20.0-35.0%, cosurfactant 0-22.0%, cosolvent 1.0-3.0%, oil 1.5-12.0% and the balance distilled water. The drug is light yellow, is clear and transparent liquid visually, has the advantages of being good in stability, low in viscosity, strong in dispersibility, rapid in absorption and the like, and further has slow releasing and targeting effects. The bioavailability of the drug can be improved, half-life period of the drug in the body can be prolonged, and curative effect is enhanced. The decoquinate nanoemulsion coccidium-resisting drug is simple in preparation process, low in energy consumption, small in toxicity and high in safety, and can be produced in a mass mode without requiring special equipment.

A method for protecting fowl against coccidiosis includes the steps of inoculating the fowl with coccidial oocysts from a strain of Eimeria that is at least partially resistant to an ionophore and subsequently administering to the fowl the ionophore and at least one chemotherapeutic anticoccidial agent. In particular, the ionophore is administered in stages both before and after the administration of the at least on chemotherapeutic agent. In a preferred embodiments, the method provides protection from coccidiosis without administration of antibiotics to the fowl and without continued administration of agents throughout the life of the fowl.

A series quinazoline ketones compounds with halogeno- quinazolone ring, propa-2-ketone group or buta-2ketone group or guaiacyl are synthesized by condensing halogeno- quinazoline-4-ketone and bromium-3-(2-methoxylbenz)propa-2-ketone or 1-bromium-4-(2-methoxyl benz )buta-2-ketone at 70-80 degree C for 1-7h under existing condition of sodium hydride, potassium carbonate, potassium tert butoxide, sodium methoxide or sodium ethylate using DMSO or DMF as a dissolvent. Proved by bioactivity experiment, the compounds have anticoccidial activity.

The invention relates to a composition for preventing and curing diarrhea, a preparation method and an application of the composition. The composition for preventing and curing the diarrhea comprises anticoccidial agent and montmorillonite; and the anticoccidial agent is methyl triazone compound. The invention further provides a preparation method of the composition for preventing and curing the diarrhea and an application of the composition for preventing and curing livestock and poultry diarrhea. The composition for preventing and curing the diarrhea in the invention can simultaneously prevent and cure coccidial, bacterial, viral and trophic livestock and poultry diarrhea (such as chickens and pigs) for increasing the survival rate and the growing speed of young livestock and poultry, reducing the cultivating cost of the livestock and poultry and improving the cultivating benefit.

[Objects] An object of the present invention is to provide an orally administrable agent for medical use for animals for preventing or treating coccidiosis which is highly safe with no side effects, an agent for a food or a drink for use in the preservation of health, a feed additive, and a feed comprising the same. Another object of the present invention is to provide a method for rearing animals (particularly, livestock and poultry) using the same and a method for controlling coccidiosis using the same.[Means for Resolution] By using a fermentation product of a lactic acid bacterium and / or a whey fermentation product of a propionic acid bacterium as an active ingredient, an agent for preventing or treating coccidiosis, an agent for use in the preservation of health, and a feed additive can be provided.

The invention discloses a preparation method of an eimeria tenella recombinant polypeptide vaccine VNQS. The preparation method comprises the following steps: preparing raw materials; the method comprises the following steps: 1, cloning a VKVQ encoding gene, designing an upstream primer shown in the formula SEQ ID NO: 3 and a downstream primer shown in the formula SEQ ID NO: 4 according to a chicken coccidiosis gene sequence, carrying out PCR amplification and electrophoresis to obtain a segment of about 1500bp, carrying out ligation transformation on the product to obtain VNQS of which the sequence overall length is 1452bp and of which the sequence is shown in the formula of SEQ ID NO: 1, and carrying out subsequent related steps. The vaccine prepared by the method can effectively controlchicken bodies to be infected with eimeria tenella by immunizing eimeria tenella recombinant polypeptide vaccine VNQS eukaryotic expression plasmids and prokaryotic expression proteins, so that the use amount of anti-coccidiosis drugs in chicken farms is greatly reduced, and chicken coccidiosis is effectively controlled.

The invention relates to the technical field of animal coccidiosis vaccines, in particular to a rabbit coccidiosis live vaccine and a preparation method and application thereof, and provides a rabbitcoccidiosis live vaccine composition which comprises precocious attenuated strains of Eimeria magna, Eimeria intestinalis and Eimeria media and precocious attenuated strain of Eimeria perforans. The rabbit coccidiosis live vaccine has high immunoprotective efficacy and safety, can effectively prevent and treat rabbit coccidiosis and reduce or avoid using of coccidiostats, can remarkably reduce unfavorable impact of vaccine inoculation on growth and production performance like rabbit weight gain and has great economic benefit and wide application prospect. The invention further provides a breeding method of coccidium precocious attenuated strains. The breeding method can effectively shorten breeding period of precocious strains and improve breeding efficiency of the coccidium precocious attenuated strains, obtained precocious strains are stable in hereditary character and high in immunogenicity, and pathogenicity is remarkably lowered.

The invention discloses an application of secoisolariciresinol diglucoside in preparation of a drug with resistance to eimeria tenella. Powder is prepared from the secoisolariciresinol diglucoside and acceptable auxiliary materials, is added to feed and is mixed uniformly, and chickens are fed according to the daily amount of feed; or water-soluble secoisolariciresinol diglucoside powder is prepared from the secoisolariciresinol diglucoside and dissolved in water, and the chickens are fed according to the daily drinking amount. Animal infection experiments prove that the ACI (anti-coccidial index) of the secoisolariciresinol diglucoside can be up to 165 or higher, and the secoisolariciresinol diglucoside has a better eimeria tenella resistant effect. Compared with a conventional anti-coccidial drug, namely, sulfachloropyrazin, the secoisolariciresinol diglucoside has a remarkable eimeria tenella resistant effect. According to the application of the secoisolariciresinol diglucoside, the problem of severe drug tolerance produced through adoption of the anti-coccidial drug in the current breeding industry can be effectively solved, the research and development speed of a new drug can be increased on the basis, and great benefits are provided for green and healthy development of the breeding industry.

The invention discloses preparation of a chicken eimeria tenella strain with EYFP knocked into an Et.Actin gene. According to the invention, an FnCas12a protein, crRNA synthesized in vitro and a homologous recombination fragment (an Et.Actin gene containing a P2A-DHFR-EYFP expression cassette) are introduced into chicken eimeria tenella sporozoite by utilizing a Lonza 4D-Nucleofector nuclear transformation instrument, so that the editing of the chicken eimeria tenella is realized. The edited insect strain is screened through pyrimethamine in a chicken body to obtain a high-purity gene edited insect strain. Then, the gene edited insect strain is verified by utilizing PCR and Sanger sequencing technologies, and it is proved that the EYFP successfully marks the Et.Actin gene. After sporozoites of the strain are subjected to cell culture and a chicken is infected, cecum mucosa is taken and smeared to show that the EYFP is expressed in the whole life history of the gene edited strain. The gene editing method can be used for performing fixed-point marking on a chicken coccidiosis gene, and is beneficial to analyzing the function of the coccidiosis gene. The invention also has important significance for research and development of novel anticoccidial drugs and vaccines.

The invention discloses a dissolving agent capable of dissolving diclazuril and ethopabate simultaneously and an anticoccidial medicinal composition. The dissolving agent consists of tetrahydrofuran and dimethylfomamide, and can be used for well dissolving diclazuril and ethopabate; and the production problem that diclazuril and ethopabate are dissoluble in water while an ordinary anticoccidial premixing agent is difficult to mix uniformly in a feed is solved. The anticoccidial medicinal composition prepared by using the dissolving agent contains high concentration diclazuril and ethopabate, and has low toxicity; a solution is stable in an environment of which the temperature is over 4 DEG C; crystal substances are precipitated out in a low-temperature environment; and crystal substances can be dissolved by heating to 65 DEG C and preserving heat for 15 minutes or heating to 25 DEG C and preserving heat for 80 minutes, and the solution becomes clear. Due to the adoption of the dissolving agent, diclazuril and ethopabate can be administrated effectively by drinking water; and the dissolving agent is simple, convenient and effective, and has a wide market prospect and high economic benefit.

The invention discloses a Chicken E.tenella MA2 (Et MA2) gene, a vector thereof, a recombinant strain thereof, a protein thereof, and an application thereof. The amino acid sequence of the EtMA2 protein is represented by SEQ ID No.2. Or the protein is a protein obtained after the amino acid sequence represented by SEQ ID No.2 is subjected to substitution, deletion, and / or addition of one or more amino acids, and / or a protein with terminal modification and with a same activity. The invention also discloses an EtMA2-outsidedomain protein with an amino acid sequence represented by SEQ ID No.4. Or the protein is a protein obtained after the amino acid sequence represented by SEQ ID No.4 is subjected to substitution, deletion, and / or addition of one or more amino acids, and / or a protein with terminal modification and with a same activity. The EtMA2 protein and the EtMA2-outsidedomain protein can be used for preparing eimeria-resisting medicines. Through the immunization of the EtMA2-outsidedomain protein, chicken can be effectively controlled from being infected by E.tenella. Therefore, the dosages of anticoccidial drugs in chicken farms can be greatly reduced, and chicken coccidiosis can be effectively controlled.

The invention relates to a method for detecting the content of six anticoccidial drugs, namely clopidol, dinitolmide, nicarbazin, sulfaquinoxaline, chlorcucurbita hydrochloride and diclazuril in chicken feed, premix and feed additives. The method mainly comprises the following steps that: extraction isperformed with using 0.1% trichloroacetic acid acetonitrile; purification is performed with 50mg of PSA; redissolving is performed with methanol and a 5mmol / L ammonium acetate aqueous solution in a volume ratio of 8: 2 as a redissolving solution; and an Agilent Extend-C18 (250 mm * 4.6 mm i. D., 5 [mu] m) chromatographic column is used as an analytical column, 270 nm and 340 nm wavelengths are selected, the sample size is 20 [mu] L, the column temperature is 25 DEG C, and an Agilent 1260 high performance liquid chromatography system (Agilent, DAD) is used for analysis. The invention aims to establish a novel, simple and convenient analysis method for rapidly determining various anticoccidial drugs in chicken feed, premix and feed additive by a high performance liquid chromatography detection method with good repeatability. The method is simple and convenient to operate and good in repeatability, and saves time and cost.

The present invention discloses a fluoroethyl thiamine or salts thereof and application thereof in preparation of anticoccidial drugs. The structural formula of the fluoroethyl thiamine or salts thereof is shown as Formula (I). The fluoroethyl thiamine or salts thereof of the present invention have a remarkable anticoccidial effect, particularly on some coccidia which had resistance to other anticoccidial drugs, therefore the fluoroethyl thiamine or salts thereof of the present invention can be applied to preparation of anticoccidial drugs. Thus, the present invention provides conditions for development of new anticoccidial drugs.

The invention belongs to the technical field of veterinary anticoccidial drugs and preparation methods thereof and discloses bacitracin methylene disalicylate- and dinitolmide-containing soluble powder and a preparation method thereof. The bacitracin methylene disalicylate- and dinitolmide-containing soluble powder comprises (a), a bacitracin methylene disalicylate raw drug, (b), a dinitolmide raw drug and (c), accessory materials. The bacitracin methylene disalicylate- and dinitolmide-containing soluble powder is prepared by a mixing method and is off-white power. The bacitracin methylene disalicylate- and dinitolmide-containing soluble powder has good stability and good palatability and can be used as a veterinary oral preparation.

The invention relates to a preparation method of a compound high-efficiency anticoccidial medicine sulfachloropyrazine sodium and diaveridine solution. The solution is provided mainly for overcoming defects such as mixing difficulty, inconvenient dosing, inaccurate dose, and the like of existing powders. The solution comprises main components of: main medicines of sulfachloropyrazine sodium and diaveridine, and auxiliary materials of dimethyl sulfoxide, N-methylpyrrolidone, a cosolvent, and triethanolamine. A preparation process provided by the invention comprises the steps that: dimethyl sulfoxide, N-methylpyrrolidone, and sulfachloropyrazine sodium are dissolved in a liquid blending tank; diaveridine well dissolved by the cosolvent is added; and triethanolamine with a formulated dose is added. The method has the advantages of simple production process, stable product quality, simple feeding method, and accurate dose.

The invention discloses application of corosolic acid and derivatives thereof in the preparation of medicines for resisting eimeria tenella. Cell level tests and animal tests are carried out to test the effect of corosolic acid on resisting eimeria tenella infection, and results show that corosolic acid has a remarkable eimeria tenella inhibiting effect, the insect resisting index of corosolic acid reaches 177.5, and the insect resisting effect of corosolic acid is remarkably superior to that of conventional insect resisting medicines including sulfachloropyrazine sodium and diclazuril. The invention discloses new application of corosolic acid in treatment of eimeria tenella infection, development and application of corosolic acid are promoted, and development and utilization of new drugsfor resisting eimeria tenella are also promoted.

The invention discloses a preparation method of an eimeria tenella recombinant polypeptide vaccine VKVQ. The preparation method comprises the following steps: 1, cloning a VKVQ encoding gene, designing an upstream primer shown in the formula SEQ ID NO: 3 and a downstream primer shown in the formula SEQ ID NO: 4 according to a chicken coccidiosis gene sequence, carrying out PCR amplification and electrophoresis to obtain a segment of about 1200bp, carrying out ligation transformation on the product to obtain VKVQ of which the sequence overall length is 1245bp and of which the sequence is shownin the formula of SEQ ID NO: 1, and carrying out subsequent related steps. The vaccine prepared by the method can effectively control chicken bodies to be infected with eimeria tenella by immunizing eimeria tenella recombinant polypeptide vaccine VKVQ eukaryotic expression plasmids and prokaryotic expression proteins, so that the use amount of anti-coccidiosis drugs in chicken farms is greatly reduced, and chicken coccidiosis is effectively controlled.

Compounds described by the Formula (I) or (II): or pharmaceutically acceptable salts, or N-oxides thereof. The compounds are useful for the treatment and prevention of protozoal diseases in mammals and birds. A method for controlling coccidiosis in poultry comprises administering an effective amount of the compound alone, or in combination with one or more anticoccidieal agent(s). A composition for controlling coccidiosis in poultry comprises the compound alone, or in combination with one or more anticoccidial agent(s). Methods for the treatment and prevention of mammalian protozoal diseases, such as, for example, toxoplasmosis, malaria. African typanosomiasis, Chagas disease, and opportunistic infections comprise administering the compound alone, or in combination with one or more antiprotozoal agent(s).

The present invention discloses a fluoroethyl thiamine or salts thereof and application thereof in preparation of anticoccidial drugs. The structural formula of the fluoroethyl thiamine or salts thereof is shown as Formula (I). The fluoroethyl thiamine or salts thereof of the present invention have a remarkable anticoccidial effect, particularly on some coccidia which had resistance to other anticoccidial drugs, therefore the fluoroethyl thiamine or salts thereof of the present invention can be applied to preparation of anticoccidial drugs. Thus, the present invention provides conditions for development of new anticoccidial drugs.

The invention discloses a compound solution of toltrazuril and diclazuril and a preparation method and application thereof, belonging to the field of livestock / poultry coccidiosis prevention. The compound solution of toltrazuril and diclazuril, disclosed by the invention, consists of main medicines and an accessory, wherein the main medicines include toltrazuril and diclazuril; preferably the mass ratio of the diclazuril to the toltrazuril is 1:(3-5), and the content of toltrazuril accounts for 2-10% of the solution by mass; the accessory is a compound solvent consisting of ethanol, diethanol amine and propylene glycol; the diethanol amine is a pH regulator which regulates the pH value of the compound solution to 8-10; and the propylene glycol is a diluting solvent. According to the prepared compound solution, the actual use concentration of toltrazuril is greatly reduced, and the cost is lowered; and the detection result also shows that the prepared compound solution is an efficient anticoccidial drug and has good stability.

The invention discloses a coccidia cell culture model. The coccidia cell culture model is built through the processes that sporozoites of Eimeria tenella are inoculated into DF-1 cells of a Chicken Embryo Fibroblast Cell line and grow into first generation merozoites. The invention also discloses an application of the coccidia cell culture model in a selection of anticoccidial drugs. The coccidia cell culture model can be utilized for establishment of a test method for measuring objectively sporozoite invasion activity, and is suitable for a selection of anticoccidial drugs and researches of gene or protein functions during a coccidia growth process.

The invention relates to use of galangin and a derivative thereof in preparing a medicine or a feed for resisting coccidiosis. Compared with the prior art, the galangin and the derivative thereof have an obvious inhibiting effect on eimeria tenella. The inhibiting effect is reflected in that the galangin and the derivative thereof can effectively inhibit reproduction of eimeria tenella on a cell culture model of the eimeria tenella. Besides, the inhibiting effect is verified by an animal body test. Aiming at the discovery of the galangin and the derivative thereof, use fields of the galangin and the derivative thereof are widened, and a new scheme is provided for development of anticoccidial drugs.

The invention belongs to the technical field of coccidium drug-resistant strain induction and acquisition, and particularly relates to a test for rapidly inducing drug-resistant strains in a simulated field and a separation and identification scheme of the drug-resistant strain, which are related to an anticoccidial drug. According to the method for simulating field rapid induction of the anti-coccidiosis drug-resistant strains, by designing two batches of rapid induction drug-resistant strain tests before and after, a plurality of stable drug-resistant strains can be rapidly screened out within 7 weeks, and then a sufficient number of drug-resistant strains can be obtained within a short time. According to the test, under the condition that the original strain background is clear, the coccidium drug-resistant strains can be rapidly screened, high timeliness is achieved, the sample number required by subsequent deep analysis is met, the reliability of the method is verified in the test for screening the drug-resistant strains of the two drugs, and the method has the advantages of being short in consumed time, easy to operate and low in cost and can be widely applied to test induction, screening and identification of chicken coccidiosis drug-resistant strains.

The invention relates to the technical field of animal disease prevention and control, in particular to a novel application of cynarin, and particularly relates to an application of cynarin in preparation of drugs or feeds for resisting coccidiosis. Compared with the prior art, the invention has the following beneficial effects that cynarin has an obvious inhibition effect on coccidium (especially eimeria tenella), and the inhibition effect is reflected in that the cynarin can effectively inhibit the reproduction of the eimeria tenella on an MDBK cell culture model of the eimeria tenella. Besides, the inhibition effect is verified by animal body tests. With the novel application of cynarin, the application field of cynarin is widened, and a new scheme is provided for development of anticoccidial drugs.

Compounds described by the Formula (I): (I) or pharmaceutically acceptable salts, or N-oxides thereof. The compounds are useful for the treatment and prevention of protozoal diseases in mammals and birds. A method for controlling coccidiosis in poultry comprises administering an effective amount of the compound alone, or in combination with one or more anticoccidial agent(s). A composition for controlling coccidiosis in poultry comprises the compound alone, or in combination with one or more anticoccidial agent(s). Methods for the treatment and prevention of mammalian protozoal diseases, such as, for example, toxoplasmosis, malaria, African trypanosomiasis, Chagas disease, and opportunistic infections comprise administering the compound alone, or in combination with one or more antiprotozoal agent(s).

The invention relates to a preparation method of a stable triazinone anticoccidial drug dry suspension. The triazinone anticoccidial drug is uniformly dispersed in a drug auxiliary material and a carrier through optimization of a suspending aid, a surfactant and a dispersing agent, the dry suspension is prepared through an extrusion spheronization process, and the preparation can be uniformly suspended in water according to a certain proportion for animals to drink. All auxiliary materials involved in the invention are easy to obtain, the cost is low, the preparation process is simple, the product quality is stable, the suspension effect in water is good and the like, and compared with a premix and a solution, the clinical application advantages are more obvious.