79results about How to "Promote endocytosis" patented technology

The successful transfer of therapeutic agents such as genetic materials (e.g. nucleic acid) or drug into living cells is the most important issue depending on the development of the delivery carrier. A method for manufacturing superparamagnetic nanoparticles in medical therapeutics is described to develop nano-sized calcium phosphate (CaP) mineral was rendered magnetic as delivery vehicle. The CaP-based magnetized nanoparticles (NPs) were possessed superparamagnetic property by hetero-epitaxial growth of magnetite on the CaP crystallites and also showed no harm to the cultured cells and elicited no cytotoxicity. The magnetized CaP was demonstrated to have good plasmid DNA binding affinity or drug carrying capacity. It significantly increased the expression of gene transfection and efficiency in delivery to mesenchymal stem cells (MSCs) under exogenous magnetic field. According to the above facts, this newly-synthesized magnetized CaP NPs has great potential as a novel non-viral targeted delivery vehicle to be applied for medical applications.

A composition for delivering an agent to a cell, comprising a bispecific affinity reagent and a pH-responsive, membrane destabilizing polymer. The bispecific affinity reagent may include a first affinity reagent covalently linked to a second affinity reagent, wherein the first affinity reagent binds to a molecule on the surface of a cell, and the second affinity reagent binds to an intracellular target.

The invention relates to a gold-gadolinium composite nano material as well as a preparation method and application of the gold-gadolinium composite nano material. The gold-gadolinium composite nano material provided by the invention comprises an inner core of a gold nano rod modified by silicon dioxide, and a gadolinium-containing silicon dioxide layer covering the outer side of the inner core. The gold-gadolinium composite nano material is high in gadolinium contain and each nano particle contains 1.5*10<6> Gd ions; the gold-gadolinium composite nano material is good in imaging effect and small in toxic side effect and can be used for loading a plurality of types of drugs and probe molecules, and target molecules can be adsorbed on the surface of the gold-gadolinium composite nano material, so that the drugs and the molecules can be efficiently transported to focus parts and multifunctional diagnosis and treatment can be realized. After the gold-gadolinium composite nano material is used as a carrier for loading the drugs, the gold-gadolinium composite nano material has the synergistic effect of thermal therapy and chemical therapy under laser irradiation in a treatment process of tumor-bearing mice, so that the growth of tumors is effectively inhibited; the gold-gadolinium composite nano material has multiple imaging functions including CT (Computed Tomography), MRI (Magnetic Resonance Imaging), photo-acoustic imaging and the like, and is suitable for being applied to complicated need tumor diagnosis and treatment.

The invention discloses a nano hydroxyapatite-gene-medicament complex as well as a preparation method and application thereof, belonging to the field of biomedicine. The complex disclosed by the invention comprises nano hydroxyapatite, anti-tumor genes and an anti-tumor medicament, wherein the size of nano hydroxyapatite is 20-300nm, the genes are anti-tumor genes and the medicament is an anti-tumor medicament. The preparation method comprises the following steps: mixing a calcium salt with a phosphate solution to prepare nano hydroxyapatite, adsorbing the anti-tumor genes to form a nano hydroxyapatite-gene precursor complex and further adsorbing the anti-tumor medicament by the precursor complex to form the complex, wherein the complex can efficiently pass through cancer cell membranes and promote the apoptosis of cancer cells. Hydroxyapatite with good biocompatibility is used as a co-transfer carrier, thereby having high safety, high loading rate of the genes and the medicament, high transfection efficiency and great effect of killing the cancer cells. Taking nano-hydroxyapatite as the co-transfer carrier of the genes and the medicament has great application prospects in the field of treatment of cancers.

A composition for delivering an agent to a cell, comprising a bispecific affinity reagent and a pH-responsive, membrane destabilizing polymer. The bispecific affinity reagent may include a first affinity reagent covalently linked to a second affinity reagent, wherein the first affinity reagent binds to a molecule on the surface of a cell, and the second affinity reagent binds to an intracellular target.

A composition for delivering an agent to a cell, comprising a bispecific affinity reagent and a pH-responsive, membrane destabilizing polymer. The bispecific affinity reagent may include a first affinity reagent covalently linked to a second affinity reagent, wherein the first affinity reagent binds to a molecule on the surface of a cell, and the second affinity reagent binds to an intracellular target.

The invention relates to a polymer vesicle of a double-loading anthracycline ring medicine and a photosensitizer, having a bubble generation function, as well as preparation. A pH / temperature double-sensitive multifunctional polymer vesicle of hydrophilic inner cavity loaded anthracycline ring medicine and a hydrophobic film loaded photosensitizer, having the bubble generation function, is prepared by taking an amphiphilic triblock copolymer PCL-b-PEG-b-PCL as a material. The anthracycline ring medicine is wrapped with and loaded in the hydrophilic inner cavity by an ammonium hydrogen carbonate gradient active medicine loading method, ammonium hydrogen carbonate can be decomposed under the condition of low pH value or heating to generate carbon dioxide bubbles, the structure of the vesicleis broken and the wrapped medicines are released rapidly. After the medicines are delivered to the local part of tumor through EPR (enhanced permeability and retention) targeting, the therapeutic effect of the anthracene ring chemotherapeutic medicines on the tumor can be obviously improved under illumination. The polymer vesicle can serve as a medicine carrier to wrap and load hydrophilic and hydrophobic medicines simultaneously, has a stable structure, has high biocompatibility and degradability, and has a wide application prospect on the aspects of medicine delivery and controllable release and chemotherapeutic combined photothermal therapy on the tumor.

The invention provides a salidroside segmented copolymer lipid nanoparticle preparation for intravenous injection and a preparation method of the nanoparticle preparation, as well as application of the preparation in preparing anti-cancer medicaments. The preparation comprises salidroside, segmented copolymer, lipid and surfactant.

The invention relates to a chitosan-deoxyribonucleic acid (DNA) nanometer granule complex and a preparation method thereof. The complex is formed by combining NH3<+> converted from amino protons on chitosan with phosphate groups on sliverlight protein granule DNA under the static action, wherein the molar ratio of N to P is 4 to 8. The complex can protect DNA from being degraded by nuclease and generate a static adsorption effect together with electronegative cell membranes to promote the endocytosis of cells and realize the transfection of target genes. Due to low cytotoxicity, the complex can be used as an excellent gene vector for gene transfection. Therefore, the transfection of the target genes is realized, and a forceful condition is provided for the application of gene therapy.

The invention relates to an oridonin cubic liquid crystal nanoparticle and a preparation method thereof. The oridonin cubic liquid crystal nanoparticle is prepared from the following raw materials in percentage by weight: 0.1 to 0.5 percent of oridonin, 7 to 64 percent of amphiphilic lipid material, 6 to 29 percent of solvent, 1 to 8 percent of stabilizer, and 25 to 65 percent of water, wherein the amphiphilic lipid material is glycerol mono-oleate or phytould likeriol; the mass ratio of a liquid crystal material to the stabilizer is 1 to (0.01 to 0.30). The oridonin cubic liquid crystal nanoparticle provided by the invention is smaller in particle size, good in uniformity, and beneficial to endocytosis and transfer of enterocyte; by utilizing a unique structure of cubic liquid crystal, a dissolution barrier and a permeation barrier of the oridonin during an oral absorption process are effectively overcome, the oral relative bioavailability of the oridonin is remarkably improved, and meanwhile, the oridonin can be slowly released.

The invention provides a nano vaccine and a preparation method thereof. The nano vaccine comprises a positively charged carrier particle and a negatively charged wrapper loaded on the surface of the carrier particle; the carrier particle is a nano-calcium carbonate particle with the surface adsorbed with a cationic polymer; the wrapper is an antigen and an adjuvant. Accordingly, the cationic polymer modified nano-calcium carbonate particle serves as an electropositive vaccine carrier to load the negative antigen and the adjuvant, and the endocytosis efficiency of the antigen and the adjuvant can be effectively improved; meanwhile, dendritic cells can be effectively activated, and the in vivo immune response is activated.

The invention discloses a quaternary ammonium type cationic starch gene controlled release vector material, and a preparation method and an application thereof; the preparation method particularly comprises the following steps: allowing starch which has a water content of 4.7-13.5% and a weight-average molecular weight of 13000-64000 Da to mix and react with a mixed system of solid caustic soda, water, and a quaternary ammonium type cationic etherifying agent which are stirred and well mixed through an ice water bath in a kneader by a dry method under a condition with a temperature of 25-30 DEG C and a rotating speed of 40-100 r / min for 12-48 hours; after the reaction is completed, washing, drying, and crushing to obtain the quaternary ammonium type cationic starch gene controlled releasevector material. An application of the vector material prepared by the invention as a non-viral vector material in gene therapy realizes the controlled release of gene drugs, improves the drug bioavailability, reduces the toxic and side effect of the drugs and the vector.

The invention relates to the technical field of medicines, and discloses a temperature targeting-based nanogel plasmid compound used for delivering expression gene medicines, and a preparation method thereof. A temperature-sensitive core-shell structured nanogel is synthesized through a free radical coprecipitation technology, and the above carrier adopts temperature-sensitive polyisopropyl acrylamide (PNIPAM) as a core and positively charged polyethyleneimine (PEI) as a shell; and a targeting intelligent nanogel carrier material with high clinic application prospect is prepared through electrostatic adsorption and gene compounding by using high-temperature passive targeting in tumor microenvironment to realize good treatment of tumors by gene medicines. The compound makes a gene carrying administration system be well enriched in a tumor position to improve the gene transfection efficiency, and provides a new treatment mode for gene treatment of tumors.

The invention discloses a nano vesicle capable of co-transporting drugs and genes, a manufacturing method and applications thereof. The component of the nano vesicle is polyethyleneimine-b-polyaspartic acid (diisoprylamino ethylamine / cysteine). The preparation method comprises the following steps: using n-butylamine to induce aspartate benzyl-N-carboxyl anhydride to carry out ring-opening polymerization reactions so as to obtain poly(aspartate benzyl), subjecting the terminal amino groups to carboxylation reactions, then mixing the reaction products with diisoprylamino ethylamine and cysteine to carry out ammonolysis reactions so as to obtain polyethyleneimine-b-polyaspartic acid (diisoprylamino ethylamine / cysteine) with carboxylated terminals, and finally subjecting the polyethyleneimine-b-polyaspartic acid (diisoprylamino ethylamine / cysteine) with carboxylated terminals to amidation reactions so as to obtain the polyethyleneimine-b-polyaspartic acid (diisoprylamino ethylamine / cysteine). The nano vesicle can be taken as the co-transportation carrier for hydrophilic drugs and genes, the hydrophobic layer of the vesicle has an acid-response property, at the same time, the disulfide bond, which is used for the binding and crosslinking of hydrophobic layer, has a reduction sensitivity, and the outer layer of the vesicle namely the polyethyleneimine layer has a proton-buffering effect, so the vesicle can release the genes and drugs intelligently.

The invention relates to a HA-targeted layered doubled hydroxide-ultrafine iron nano material and preparation and applications thereof. The preparation is as follows: synthesizing an LDH-Fe3O4 nano composite material by a coprecipitation method; covalently bonding activated hyaluronic acid on the surface of an LDH laminate through a silane coupling agent; and finally, carrying out surface loadingof an anticancer drug. The nano-material particles are uniformly distributed, and can enhance the MR imaging effect of the tumor part in an animal body. The LDH-Fe3O4-HA NPs is used as a carrier of ananticancer drug doxorubicin DOX, not only has sensitive pH response and release characteristics, but also can carry out specific recognition on tumor cells expressed by CD44 receptors so as to achieve the idealization effect of efficiently inhibiting tumors.

The invention provides UV photoresponsive hyperbranched poly(belta-amino esters) with the efficient gene delivery capability, and a preparation method and application thereof. The polymer is polymerized by an 'A2+B3+C2' Michael addition method, and thus the polymer is provided with a hyperbranched structure. Compared with a linear structure, the branched structure can enhance the interaction between the polymer and nucleic acid molecules, significantly improves the gene condensation capability, and meanwhile can increase cell uptake by enhancing the interaction with a cell membrane. A main chain of the polymer is provided with a UV responsive group, and under UV light irradiation, the poly(belta-amino esters) can be rapidly degraded after cell endocytosis to release an entrapment gene; andefficient gene transfection is realized, and material toxicity is lowered. The properties of the poly(belta-amino esters) enable the poly(belta-amino esters) to have great development prospects in the field of biomedical materials, especially the field of gene delivery.

The invention relates to antitumor application of chlorambucil-polydopamine prodrug nanoparticles, and concretely relates to a mild photothermal therapy-chemotherapy combined tumor alete treatment technology based on the chlorambucil-polydopamine prodrug nanoparticles. The nanoparticles can realize spatiotemporal manipulation in an antitumor effect in order to achieve accurately-positioned tumor ablation, and passive targeting action of the prodrug nanoparticles on tumor parts can be achieved by the enhanced permeation and retention(EPR) effect; the mild photothermal effect can enhance the expansion of tumor blood vessels and the permeability of cell membrane effectively promote the accumulation and penetration of the nanoparticles in tumors and the endocytosis of tumor cells; and photothermal therapy can induce the antitumor immunity in order to improve the effect of chemotherapy, so synergistic antitumor effects are generated in the photothermotherapy-chemotherapy combined therapy. The application provides a new way for precise cancer treatment, and has a potential clinical application prospect.

The invention discloses a conjugated backbone doped zwitterionic polyfluorene vinylene and preparation and application. The preparation comprises the following steps of: taking dibromofluorene derivative (A monomer), dienyl benzene derivative (B monomer) and dibromoaromatic heterocyclic structure (C monomer) doped with a small amount of narrow band gap, adopting a three-component polymerization method of A+B+C to carry out the Heck reaction of the organic metal catalysis, obtaining a neutral polymer, and obtaining the conjugated backbone doped zwitterionic water-soluble polyfluorene vinylene subjected to the reaction of quaternary and / or acid ester hydrolysis and the like. The synthesis method is simple, and the ratio type fluorescence emission can be achieved with improved detection sensitivity; and the red / near-infrared emission can be achieved, and the fluorescence biological imaging effect can be improved. The zwitterionic structure is beneficial to the endocytosis of the cells, which can effectively improve the adsorption of the material by the cell membrane, so that the better biological imaging effect can be achieved.

The present invention relates to a cell-nucleus-targeted antitumor nanomedicine carrier and a preparation method and application thereof. The cell-nucleus-targeted antitumor nanomedicine carrier comprises polymer micelle NLS-PEG-PAsp (BzA) and a pH-sensitive negatively-charged polymer constructed on the surface of the polymer micelle by electrostatic interaction, the pH-sensitive negatively-charged polymer can changed into positively-charged from negatively-charged when pH is 6.5 to 6.8, the size of the polymer micelle is 20nm-40nm, and the particle size of the nanomedicine carrier is 100nm-110nm. The cell-nucleus-targeted antitumor nanomedicine carrier can stably circulate in body and is effectively enriched in a tumor site through EPR effect. When a nano medicine reaches tumor microenvironment, under the condition of the pH of 6.5-6.8, a negative polymer protective layer compounded on the surface of the polymer micelle is changed into positively-charged from negatively-charged, and further is removed from the surface of nanoparticles of the polymer micelle, the positively-charged polymer micelle facilitates endocytosis, and finally the positively-charged polymer micelle targets tumor nuclei under the action of nuclear localization signal peptide to release the antitumor medicine in the nucleus.

The invention relates to a folate-receptor-mediated pH-sensitive Decoy-ODN (Decoy-oligodeoxynucleotide) nanoparticle preparation and a preparation method thereof. A nanoparticle suspension is prepared from 5-200 mu g / ml of the Decoy-ODN used as a generic medicament, 0.01-6.0 mg / ml of folate-conjugated trimethyl chitosan used as a medicament carrier and 10-500 mu g / ml of pH-sensitive material by a complex coacervation method, wherein the folate conjugation rate of the folate-conjugated trimethyl chitosan is 5-30%, and the ratio of the total volume of the Decoy-ODN solution and the pH-sensitive material solution to the volume of the folate-conjugated trimethyl chitosan solution is 1:(1-4). The folate-receptor-mediated pH-sensitive Decoy-ODN nanoparticle preparation provided by the invention has the advantages of good shaping property, uniform particle size distribution, high DNA (deoxyribonucleic acid) binding rate, better stability, low cytotoxicity and high in-vitro transfection efficiency.

The invention discloses an anti-human ErbB2 bispecific antibody as well as a preparation method and application thereof. The anti-human ErbB2 bispecific antibody provided by the invention is prepared by linking a single chain antibody of anti-human ErbB2 to the N-terminal of the heavy chain of the Trastuzumab antibody through linker peptides; the heavy chain variable region in the single-chain antibody is a heavy chain variable region of an HuA21 antibody; a light chain variable region in the single chain antibody is a light chain variable region of an HuA21 antibody; the amino acid sequence of the linker peptides is the 275th to 289th sites in the sequence I of the sequence table. The anti-human ErbB2 bispecific antibody provided by the invention has a good antitumor effect and has a more excellent endocytosis effect than the original Trastuzumab antibody and the HuA21 antibody, so that the anti-human ErbB2 bispecific antibody is of great significance as used for preparing antibody targeting drugs as a drug carrying tool.

Provided is a method of preparing a stimuli-responsive multifunctional nanoparticle, including in sequence the steps of: (a) conjugating covalently an active targeting moiety to a hydrophilic polymer to form a targeted polymer, (b) conjugating covalently a redox-responsive moiety to the hydrophilic polymer of the targeted polymer to form a targeted redox-responsive polymer, (c) conjugating covalently a pH-responsive moiety of a drug complex to the redox-responsive moiety of the targeted redox-responsive polymer to form a targeted stimuli-responsive polymer-drug conjugate, wherein the drug complex includes a hydrophobic drug covalently linked to the pH-responsive moiety, and (d) adding the targeted stimuli-responsive polymer-drug conjugate and optionally an imaging agent into an aqueous liquid to allow self-assembly into a stimuli-responsive multifunctional nanoparticle, wherein the hydrophobic drug of the stimuli-responsive multifunctional nanoparticle forms a hydrophobic core, and the imaging agent is incorporated within the hydrophobic core.

The invention provides a PD-L1 antibody and a STING agonist loaded nano-vesicle, the structure of the nano-vesicle comprises a shell and an inner core, the shell is prepared from a lipid bilayer membrane, the shell is coupled with an MMP-2 sensitive polypeptide chain, the polypeptide chain is coupled with a PD-L1 antibody, and the shell is also coupled with PEG; and the inner core of the gene is an STING agonist. According to the nano-vesicle disclosed by the invention, the long-chain PEG is used as a protective barrier of an antibody, so that non-specific binding of a PD-L1 antigen on normal tissues of the PD-L1 antibody is prevented; the nano-vesicle has the pH and MMP-2 dual sensitivity, and the shell liposome is coupled with the MMP-2 sensitive peptide chain, such that the vesicle can responsively release the PEG layer and the PD-L1 antibody when the vesicle reaches the acidic and high MMP-2 concentration residual tumor micro-environment, and the surface charge of the vesicle is converted from negative to positive after the release so as to effectively promote the endocytosis of the STING activator carrier by the antigen-presenting cells.

The invention provides a lysine grafted polyethyleneimine cationic gene vector. The lysine grafted polyethyleneimine cationic gene vector comprises hyperbranched polyethyleneimine and lysine which is grafted on the hyperbranched polyethyleneimine and is protected by p-toluenesulfonyl. In order to realize high-efficiency delivery of a gene vector to a gene, polyethyleneimine with small molecular weight is modified, and lysine protected by p-toluenesulfonyl is grafted on the polyethyleneimine. According to the invention, three PEI-Lys (Tos) carriers with different grafting ratios (1: 5; 1: 10; 1: 15) are prepared. Through comparison, the grafting ratio (1: 10) with the best transfection performance is screened for other subsequent evaluation characterization. Experimental results show that the PEI-Lys (Tos) cationic carrier prepared through modification design has higher transfection efficiency and lower cytotoxicity, and has a huge application prospect in the fields of gene carrier design and tumor resistance.

The invention relates to an ofloxacin liquid crystal nano-particle eye drop and a preparation method thereof. The ofloxacin liquid crystal nano-particle eye drop is prepared from 0.12-0.2% of ofloxacin, 20-50% of at least one neutral lipid and / or tocopherol, 20-50% of at least one phosphatidylcholine, 0.2-20% of a nonionic surfactant, 0.1-10% of at least one solvent, 0.28-0.35% of at least one thickener, and the balance of injection water. The liquid crystal gel nano-particle has a small and uniform particle size, so the endocytosis and the transport of cells are benefited; and the unique adhesion and the liquid crystal phase structure of the liquid crystal gel are used to prolong the retention time of drug active ingredients in eyes and effectively control the release of an entrapped substance, so the bioavailability is improved. Additionally, the preparation method of the ofloxacin liquid crystal gel nano-particle eye drop has the advantages of simple process steps, easiness in industrial large-scale production, and low production cost.

The invention provides ultrafast charge reversible chitosan-based nanogel as well as a preparation method and application thereof, and belongs to the field of biomedicine. The preparation method comprises the following steps: synthesizing a chitosan-polypyrrole polymer, then cross-linking the chitosan-polypyrrole polymer with glutaraldehyde to prepare chitosan-polypyrrole nanogel, and finally, treating the chitosan-polypyrrole nanogel with a NaOH solution to obtain a series of chitosan-polypyrrole-hydroxyl nanogel. The prepared ultrafast charge reversible chitosan-based nanogel can realize rapid conversion of pH-induced surface charges, has good cell compatibility, good protein resistance, relatively high anti-cancer drug loading efficiency and relatively good anti-tumor ability, and is hopeful to be used as a safe and promising nano-carrier to construct a low-side-effect enhanced anti-tumor treatment platform, and is applied to the field of biomedicine.

The invention discloses the use of a protein in preparation of drugs for prevention and treatment of Alzheimer's disease. The amino acid sequence of the protein is shown as SEQ ID NO:2, the protein contains the extracellular fragment 1-171th amino acids of TREM2 receptor protein, and the nucleotide sequence is shown as SEQ ID NO:1. The protein can increase the density of microglial cells, promotethe endocytosis and degradation of microglial cells to Abeta (Amyloid-beta), reduce the intracerebral Abeta level and the deposition of Abeta amyloid plaques, increase the long-term potentiation of CA3-CA1 in the mouse hippocampus region, and strengthen the learning and memory ability of mice.

The invention relates to a preparation method for a nano drug. The method includes forming a drug-loaded nano-micelle by an amphiphilic block copolymer containing a carboxyl group in a hydrophilic chain segment and a hydrophobic drug through self-assembly; obtaining a disulfide-bonded, sulfhydryl and carboxyl bi-functionalized first organosilicon nanohybrid micelle through the reaction of the drug-loaded nano-micelle and a sulfhydryl or disulfide bond containing silane coupling agent; obtaining a polyethylene glycol and carboxyl bi-functionalized second organosilicon nanohybrid micelle throughthe oxidation reaction of the first organosilicon nanohybrid micelle; and obtaining the polyethylene glycol and polyethyleneimine bi-functionalized nano drug through the amide reaction of the secondorganosilicon nanohybrid micelle. The obtained nano drug and an application thereof are also related. The enrichment and retention of the nano drug on tumors can be promoted according to the first level response of the nano drug, and the responsive release of anti-cancer drugs can also be promoted through second level response, so that more efficient chemotherapy can be realized.

The invention discloses a water-soluble cyclodextrin drug carrier with cell targeting. The water-soluble cyclodextrin drug carrier comprises beta-cyclodextrin as an entrapment drug body, and the beta-cyclodextrin is linked with at least one choline phosphate at a fixed point. The preparation method comprises the following steps: (1) taking methanol and 2-chloro-2-oxo-1, 3, 2-dioxaphospholane as raw materials to synthesize 2-methoxy-2-oxo-1, 3, 2-dioxaphospholane; (2) synthesizing choline phosphate by taking 2-methoxy-2-oxo-1, 3, 2-dioxaphospholane and 1-dimethylamino-2-propyne as raw materials; and (3) finally, through a click reaction, enabling the mono (6-azide-6-deoxy)-beta-cyclodextrin, choline phosphate and a ligand N, N, N', N, N''-pentamethyldiethylenetriamine to react to generate the mono-substituted choline phosphate cyclodextrin. The drug carrier can improve the hydrophilicity of beta-cyclodextrin and endow the beta-cyclodextrin with cell adhesiveness by utilizing the chargeeffect between choline phosphate and a cell membrane, so that the high efficiency of drug absorption in cells is realized.

The invention relates to an ultralow-frequency-magnetic-field-sensitive functional magnetic nano material and a preparation method thereof. The preparation method of the functional magnetic nano material comprises the following steps: (1) preparing a magnetic nano material from zinc acetylacetonate and iron acetylacetonate; and (2) improving the water solubility of the prepared magnetic nano material, and modifying targeting groups onto the surface of the water-soluble magnetic nano material, thereby obtaining the functional magnetic nano material. Compared with the prior art, the functional magnetic nano material is sensitive to the ultralow-frequency rotating magnetic field, and can avoid the adverse effects of the high-frequency magnetic field on the human body. After being modified by the targeting groups, the functional magnetic nano material has the function of targeting tumor cells, and can kill the tumor cell safely and effectively under the action of the ultralow-frequency magnetic field. The type of the targeting groups can be changed to implement accurate treatment on different cancer cells and tumor tissues, and the treatment process is safe and effective.