41 results about "Mouse Hippocampus" patented technology

The invention discloses a traditional Chinese medicine composition, a preparation method and application thereof. The traditional Chinese medicine composition is composed of Coptidis Rhizoma Cortex Phellodendron, Cortex Phellodendri, Gardenia, Salvia Miltiorrhiza, Curcuma longa and Shichangpu. The traditional Chinese medicine composition can significantly improve the learning and memory dysfunction of 5×FAD transgenic mice, reduce the expression of Aβ40 / 42 in the hippocampus, and increase the 5 The expression levels of pre- and post-synaptic proteins in the hippocampus of ×FAD transgenic mice can improve the excitatory synaptic transmission disorder in the hippocampus of 5×FAD mice, and at the same time inhibit the apoptosis of hippocampal neurons and the proliferation of microglia in 5×FAD mice In addition, it can inhibit the expression of inflammatory factors in the serum and hippocampus of 5×FAD mice, which provides a reliable theoretical basis for the preparation of drugs for the treatment or adjuvant treatment of Alzheimer's disease.

The invention provides a traditional Chinese medicine composition for improving memory and mild cognitive impairment. The traditional Chinese medicine composition comprises 40-80 parts of thinleaf milkwort root-bark, 40-80 parts of grass leaf sweetflag rhizome, 40-80 parts of ginseng, 80-120 parts of tall gastrodia tuber, 80-120 parts of papermulberry fruit, 80-120 parts of ginkgo leaves and 0.01-3 parts of borneol. The traditional Chinese medicine composition can achieve the effects of obviously increasing the number density and surface density of synapse of the molecular layer of mouse hippocampal CA1, obviously enhancing the SOD activities of central cerebral cortex and hippocampus, reducing the content of lipofuscin of the neuron, obviously improving the learning and memory impairment of spontaneous senile dementia model mice, obviously enhancing the activity and fiber density of NOS1 of the central cerebral cortex and hippocampus and obviously up-regulating the contents of SS, AVP and ACTH of the cerebral cortex and hippocampus, especially the content of SS. The traditional Chinese medicine composition can achieve the effect of obviously improving the clinical symptoms of memory and cognitive impairment and has definite curative effects and great application prospects.

The invention provides a construction method and application of an AAV virus vector capable of specifically expressing Cre recombinase in the CA2 region of mouse hippocampus. The invention cuts out the partial promoter region of mouse hippocampus CA2 specific expression gene Map3k15 and connects it with Cre recombinase, and clones it into the pAAV vector without initial promoter. The AAV vector with the Map3k15 promoter linked to the Cre recombinase constructed by the present invention can effectively specifically overexpress the Cre recombinase in the CA2 region of the mouse hippocampus to achieve specific regulation of neuron activity in the CA2 region, and establishes a small gene that does not depend on the Cre transgene. The mouse CA2 neuron tracing and regulation system provides an effective tool for studying the function of the CA2 region, and provides a basis for the subsequent study of the molecular mechanism of CA2 function.

The invention discloses an auxiliary positioning device for mouse brain injection, which comprises a constant temperature base plate, a vertical axis moving scale and a clamping assembly. The four corners of the lower surface of the constant temperature base plate are threaded with non-slip feet, and the inside of the constant temperature base plate is installed with A constant temperature heating assembly, a fixed component is fixed on the rear side of the upper surface of the constant temperature bottom plate, the vertical axis moving scale is installed on the left surface of the constant temperature bottom plate, and the upper end of the vertical axis moving scale is fixed with a vertical plate, the inside of the vertical axis A screw is installed in the middle position, and the top of the screw is welded with an adjustment handle. The outer wall of the screw is threaded with a ball slide, and the right side of the ball slide is equipped with a horizontal axis moving scale, and the clamping assembly is installed on the horizontal axis. Move the ruler below. The present invention greatly improves the accuracy of the entire positioning device by adopting a moving scale with a positioning accuracy of 0.01mm, thus solving the problem in the prior art that precise positioning cannot be performed due to the small hippocampus of mice.