Application of inhibitors of miRNA-182, miRNA-188 and miRNA-199a

A technology of miRNA-199a and miRNA-188, applied in the direction of medical preparations containing active ingredients, organic active ingredients, nervous system diseases, etc., to reduce the formation of Aβ, improve plasticity, and improve the effect of learning and memory

Pending Publication Date: 2020-08-25
SHIHEZI UNIVERSITY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Various treatments for Alzheimer's disease have been developed and advanced to late clinical trials in AD patients, but so far, no drug has been proven effective in large phase III trials

Method used

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  • Application of inhibitors of miRNA-182, miRNA-188 and miRNA-199a
  • Application of inhibitors of miRNA-182, miRNA-188 and miRNA-199a
  • Application of inhibitors of miRNA-182, miRNA-188 and miRNA-199a

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Embodiment Construction

[0016] 1. Detection and determination of specifically up-regulated miRNAs in AD model mice

[0017] 1. Take materials from hippocampus and cortex

[0018] Animals were sacrificed by cervical dislocation, and brain tissues were removed. The brain tissue was washed with saline at 4°C, and then dried with absorbent paper. The left and right hippocampus and cerebral cortex were taken out, rinsed with 4°C saline, dried with absorbent paper, and numbered.

[0019] 2. RNA extraction and detection

[0020] 3. First-strand cDNA reverse transcription

[0021] 1) The specific primers (designed by Primer Premier 5.0 software) designed for U6 and other miRNAs used in the reverse transcription of the first-strand cDNA, the primer sequences are shown in Table 1:

[0022] Table 1 Primer name and sequence

[0023]

[0024] 2) Add the following reagents to the nuclease-free PCR tube in ice bath, as shown in Table 2:

[0025] Table 2 PCR reaction system

[0026]

[0027] 3) Gently m...

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Abstract

The invention relates to the technical field of biomedicine technology, and particularly discloses application of inhibitors of miRNA-182, miRNA-188 and miRNA-199a. The therapeutic effect of a miRNA inhibitor AM-182 / 188 / 199a on AD is clarified, specifically, the neuronal function of the hippocampus of AD mice and the plasticity of neuronal neurites are improved, the formation of A[beta] is reduced, the learning and memory ability of the AD mice are improved, new prevention and treatment methods are provided for the clinical treatment of AD, and new ideas are provided for the preparation of drugs for the treatment of AD.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of inhibitors of miRNA-182, miRNA-188 and miRNA-199a. Background technique [0002] Alzheimer's disease (AD) is a neurodegenerative disease characterized by impairment of learning and memory. The accumulation of β-amyloid protein and hyperphosphorylated tau protein, acetylcholine neurotransmitter, occurs in the brain of patients. reduction in cellular and molecular changes such as inflammation and oxidative stress. The accumulation of β-amyloid (Aβ) in extracellular plaques and hyperphosphorylated tau protein leading to intracellular neurofibrillary tangles are the most typical pathological features of AD. Various treatments for Alzheimer's disease have been developed and advanced to late clinical trials in AD patients, but so far, no drug has been proven effective in large phase III trials. AD drug development failure rate is 99%; AD disease-modifying thera...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7105A61P25/28
CPCA61K31/7105A61P25/28
Inventor 黄瑾杨磊朱井玲宋丹丹孙嘉伟
Owner SHIHEZI UNIVERSITY
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