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Lactobacillus rhamnosus CCFM1228 with functions of relieving depressive emotion and promoting intestinal tract to secrete IgA (Immunoglobulin A) and application of lactobacillus rhamnosus CCFM1228

A technology of CCFM1228 and Lactobacillus rhamnosus, applied in the field of microorganisms, can solve the problems of inconsistent treatment effects, large side effects, and slow onset of antidepressant drugs, and achieve the goal of increasing intestinal IgA levels, reducing activity, and increasing IgA content Effect

Pending Publication Date: 2022-05-27
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Antidepressants currently widely used in clinical treatment generally have the disadvantages of slow onset of action, inconsistent treatment effects, and large side effects, which make the treatment of depression have serious limitations.

Method used

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  • Lactobacillus rhamnosus CCFM1228 with functions of relieving depressive emotion and promoting intestinal tract to secrete IgA (Immunoglobulin A) and application of lactobacillus rhamnosus CCFM1228
  • Lactobacillus rhamnosus CCFM1228 with functions of relieving depressive emotion and promoting intestinal tract to secrete IgA (Immunoglobulin A) and application of lactobacillus rhamnosus CCFM1228
  • Lactobacillus rhamnosus CCFM1228 with functions of relieving depressive emotion and promoting intestinal tract to secrete IgA (Immunoglobulin A) and application of lactobacillus rhamnosus CCFM1228

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0109] Example 1: Acquisition and identification of Lactobacillus rhamnosus CCFM1228

[0110] 1. Isolation and screening of Lactobacillus rhamnosus

[0111] (l) Take 1 g of the feces of a healthy person. After gradient dilution, it was coated on MRS solid medium and placed in an anaerobic environment at 37°C for 72 hours; the colony morphology was observed and recorded, and the colonies were picked for streak purification; cultured in MRS liquid medium at 37°C for 48 hours, the obtained colonies Gram staining was performed and colony morphology was recorded. Gram-negative bacterial strains and Gram-positive cocci in the colony were discarded, and Gram-positive bacilli were selected.

[0112] (2) After the catalase analysis, the catalase-positive strains were discarded, and the catalase-negative strains were retained.

[0113] 2. Molecular biological identification of Lactobacillus rhamnosus:

[0114] (1) Single bacterial genome extraction: The catalase-negative strain scre...

Embodiment 2

[0134] Example 2: Lactobacillus rhamnosus CCFM1228 can reduce depression-like behavior in mice

[0135] Specific steps are as follows:

[0136] (1) Lactic acid bacteria gavage:

[0137] Activation of strains:

[0138] Take Lactobacillus rhamnosus CCFM1228, Lactobacillus rhamnosus PAL1, Lactobacillus rhamnosus ATCC7469 stored in the bacterial bank and streak MRS plates respectively, and after culturing at 37 °C for 48 hours, pick a single colony from the plate to inoculate Into the corresponding 5mL MRS liquid medium, after culturing at 37°C for 24h, inoculate it into the corresponding fresh MRS liquid medium at a ratio of 2% (v / v), incubate at 37°C for 24h, and prepare rhamnosus. Lactobacillus saccharomyces CCFM1228 seed solution, Lactobacillus rhamnosus PAL1 seed solution, Lactobacillus rhamnosus ATCC7469 seed solution.

[0139] Preparation of bacterial suspension:

[0140] Take the above-mentioned activated 2-generation Lactobacillus rhamnosus CCFM1228, Lactobacillus rha...

Embodiment 3

[0156] Example 3: Lactobacillus rhamnosus CCFM1228 can significantly increase the level of neurotransmitter (5-HT) in the brain of depressed mice

[0157] DETAILED DESCRIPTION The same as in Example 2, the mice in Example 2 were euthanized at the end of the eighth week, and the mouse brain tissue was collected, and the prefrontal lobes were isolated on ice.

[0158] Take a certain quality of fresh prefrontal cortex tissue, add 30 times the volume of 0.2M perchloric acid solution (equivalent to 1 g tissue plus 30 mL of homogenate perchloric acid solution), use a tissue homogenizer for homogenization, and the tissue fluid passes through. After centrifugation at 12,000 g for 10 min, the supernatant was drawn and filtered through a 0.22 μm water filter to detect the content of 5-HT in the prefrontal cortex tissue.

[0159] The experimental results are as image 3 As shown in A, the content of 5-HT in the normal control group, depression model group, CCFM1228 intervention group, P...

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Abstract

The invention discloses lactobacillus rhamnosus CCFM1228 with functions of relieving depressive mood and promoting intestinal tract to secrete IgA and application of the lactobacillus rhamnosus CCFM1228, and belongs to the technical field of microorganisms. The lactobacillus rhamnosus can relieve depression-like behaviors of depressive mice, increase the content of neurotransmitter 5-hydroxytryptamine in the brain and increase the BDNF level in hippocampus of the depressive mice, and the lactobacillus rhamnosus CCFM1228 has the functions of relieving depressive mood and promoting intestinal tract to secrete IgA. The expression of hypothalamus Crh mRNA is inhibited, the level of corticosterone in serum is reduced, the activity of xanthine oxidase in cerebral cortex is reduced, the activity of adenosine deaminase in liver is improved, and the content of uric acid in serum is reduced; the prefrontal lobe Mbp mRNA level is improved, and the stability of the CNS myelin sheath structure and function is maintained; the mRNA level of the prefrontal lobe Gfap is improved, and the function of astrocytes is enhanced; the mRNA level of hippocampus CD36 is reduced, and immune homeostasis is regulated; the level of IgA in host feces can be improved on the basis of relieving the depression mood, and wide application prospects are achieved.

Description

technical field [0001] The invention relates to a Lactobacillus rhamnosus CCFM1228 with functions of relieving depression and promoting intestinal secretion of IgA at the same time and application thereof, belonging to the technical field of microorganisms. Background technique [0002] Depression (Major Depressive Disorder, MDD) is a common mood disorder in which patients suffer from persistent low mood, pessimism and world-weariness, deviations from cognition and healthy people, and suffer from mental pain, accompanied by physical symptoms in severe cases. People with MDD often have disrupted circadian rhythms and poor sleep quality. Studies have shown that increased frequency of insomnia is associated with increased depression and anxiety, and increased number of awakenings is also associated with increased depression. Depression is an important public health problem and one of the leading causes of the global disease burden. According to reports, about 350 million peop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P25/24A61P25/22A61P1/00A23C9/123A23C13/16A23C19/032A23C19/06A23L11/50A23L19/00A23L33/135C12R1/225
CPCC12N1/20A61K35/747A61P25/24A61P25/22A61P1/00A23C9/1234A23C13/16A23C19/0323A23C19/062A23L11/50A23L19/00A23L33/135A23V2002/00A23V2400/175A23V2200/322A23V2200/3204Y02A50/30
Inventor 王刚梅丽亚许梦舒田培郡赵建新张灏陈卫
Owner JIANGNAN UNIV
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