45 results about "Dendritic spine" patented technology

A method for visualizing an active synapse wherein said method comprises: (a) exposing cells forming the active synapse to a biomarker comprising at least fragment C of tetanus toxin and a reporter protein; and (b) visualizing the biomarker; wherein the accumulation of the biomarker into dendritic spines of the cells allows visualization of an active synapse. Also, a method for screening molecules capable of modulating synapse activity is provided. A kit useful for the early diagnosis of neurodegenerative disease comprises a biomarker comprising at least fragment C of tetanus toxin and a reporter protein.

The invention discloses a femtosecond pulse laser modulator and a fast high resolution miniature two-photon microscope having the same. The femtosecond pulse laser modulator comprises a negative dispersion optical path and a positive dispersion optical path, wherein the negative dispersion optical path comprises a laser input optical fiber for transmitting a pulse-widened prechirp compensated laser beam to a scanning imaging portion; and the positive dispersion optical path is located between a femtosecond pulse laser device and the negative dispersion optical path and is used for compensating the negative dispersion caused by the laser input optical fiber in a laser transmission process. The femtosecond pulse laser modulator is capable of providing a favorable condition for the fast high resolution miniature two-photon microscope to stably observe the dendrites and the dendritic spine of unrestrained animals in a natural physiological environment.

The invention provides a wintergreen pure polysaccharide LTC-0-1 and an application thereof, which belong to the field of medicines and healthcare foods. The invention discloses an extracting method of the wintergreen pure polysaccharide LTC-0-1, a composition of the LTC-0-1 and a molecular weight of the LTC-0-1; it is proved by experiments that the LTC-0-1 can be used for increasing average lengths of hippocampal neurons of a GFP green fluorescent protein transgenic mouse, increasing the number of the neurons, obviously increasing dendritic spine densities of neuron dendrons in a unit length, increasing neuronal post-synaptic membrane protein expression and promoting the neurons to be more mature; the LTC-0-1 can be also used for inducing neuron-like differentiation of PC12 cells, promoting enation of the cells, enhancing activity of cell acetylcholinesterase AchE and increasing expression index of cell growth related protein GAP-43 and obtains neurotrophic activity; and the LTC-0-1 shows a growth promoting effect, a differentiation effect, a maturing effect and a damaged nerve cell function recovery effect on nerve cells and has broad prospects in development and application in the technical field of medicines and healthcare foods.

The present invention provides methods of slowing or reversing the loss of memory and learning comprising the steps of contacting an effective amount of a PKC activator with a protein kinase C (PKC) in a subject identified with memory loss slowing or reversing memory loss. The present invention provides methods of stimulating cellular growth, neuronal growth, dendritic growth, dendritic spine formation, dendritic spine density, and the translocation of ELAV to proximal dendrites, and synaptic remodeling. The present invention also provides methods of contacting a protein kinase C (PKC) activator with a PKC activator in a manner sufficient to stimulate the synthesis of proteins sufficient to consolidate long-term memory. The present invention also provides methods of contacting a protein kinase C (PKC) activator with a PKC activator in a manner sufficient to downregulate PKC.

The invention discloses a nerve dendritic spine image classification method based on multiresolution fractal features. The method includes the following steps of (1) extracting features of nerve dendritic spine images to obtain the multiresolution fractal features of the nerve dendritic spine images; and (2) classifying the nerve dendritic spine images based on the multiresolution fractal features of the nerve dendritic spine images in the method of linear discriminant analysis (LDA). According to the method, classification precision is high and classification results are stable.

The present invention provides methods of slowing or reversing the loss of memory and learning comprising the steps of contacting an effective amount of a PKC activator with a protein kinase C (PKC) in a subject identified with memory loss slowing or reversing memory loss. The present invention provides methods of stimulating cellular growth, neuronal growth, dendritic growth, dendritic spine formation, dendritic spine density, and the translocation of ELAV to proximal dendrites, and synaptic remodeling. The present invention also provides methods of contacting a protein kinase C (PKC) activator with a PKC activator in a manner sufficient to stimulate the synthesis of proteins sufficient to consolidate long-term memory. The present invention also provides methods of contacting a protein kinase C (PKC) activator with a PKC activator in a manner sufficient to downregulate PKC.