The invention belongs to the technical field of water-based polyurethane paints, and discloses a water-based polyurethane paint containing a polyether glycol / nano TiO2 hybrid material, and a preparation method and application thereof. The paint comprises the following components in parts by mass: 25-75 parts of polyether glycol / nano titanium dioxide hybrid material, 15-65 parts of diisocyanate, 100 parts of polyether glycol, 8-16 parts of polyhydroxy-carboxylic acid, 0.05-0.5 part of organo-metallic catalyst, 0.5-10 parts of crosslinking agent, 6.2-12.4 parts of neutralizer, 1-8 parts of chain extender and 0.2-2.0 parts of defoaming agent. The paint enhances the compatibility between the nano titanium dioxide and polyurethane base, has the advantages of favorable thermal properties, favorable mechanical properties, favorable wear resistance and excellent ultraviolet resistance, and can be widely used in the fields of architectural paints, automobile paints, wood paints, electrodeposition paints, paper conditioning paints, glass fiber paints, electrophoretic paints and the like.