Nano vaccine and preparation method thereof

A technology of nano-vaccine and nano-calcium carbonate, which is applied in the field of nano-vaccine and its preparation, can solve the problems of limiting vaccine efficiency, low endocytosis efficiency, and inability to effectively produce anti-tumor effector T cells, so as to improve vaccine efficiency and promote activation effect

Active Publication Date: 2019-07-12
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low endocytosis efficiency of antigens and adjuvants, it still greatly limits the efficiency of the vaccine and cannot effectively generate anti-tumor effector T cells

Method used

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  • Nano vaccine and preparation method thereof
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  • Nano vaccine and preparation method thereof

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Effect test

preparation example Construction

[0046] The present invention also provides a preparation method of the nano-vaccine described in the above-mentioned technical scheme, comprising the following steps:

[0047] a) mixing the nano-calcium carbonate suspension with the cationic polymer solution and placing it aside for complex treatment, then centrifuging and washing to obtain carrier particles;

[0048] b) dispersing the carrier particles in water to obtain a carrier suspension;

[0049] c) After mixing the carrier suspension with the antigen-adjuvant mixture and standing for compound treatment, centrifuge washing and ultrasonic dispersion to obtain a nano-vaccine dispersion;

[0050] Or mix the carrier suspension with the antigen-adjuvant mixed solution and put it aside for complex treatment, then centrifuge and wash to obtain the nano vaccine powder.

[0051] Among them, the characteristics such as the type, content, particle size and source of nano-calcium carbonate, cationic polymer, antigen, and adjuvant a...

Embodiment 1

[0084] 1.1 Preparation of vaccine

[0085] Ultrasonically disperse nano-calcium carbonate in ultrapure water, the concentration of nano-calcium carbonate is 1mg / mL, and the ultrasonic time is 5min to form nano-calcium carbonate suspension. Polyethyleneimine (25000Da, PEI25K) was dissolved in ultrapure water at a concentration of 5 mg / mL to form a polyethyleneimine solution. Add the polyethylenimine solution into the nano-calcium carbonate suspension, mix equal volumes, vortex for 15 seconds, and then let stand at room temperature for 15 minutes; then centrifuge and wash at 6000r / min for 10 minutes and wash twice to obtain the carrier particles.

[0086] The carrier was dispersed in ultrapure water at a concentration of 1 mg / mL. After that, add an equal volume of antigen-adjuvant mixture, the antigen is a commercially available model antigen (ovalbumin OVA antigen), the adjuvant is oligonucleotide CpG, and the mass ratio of carrier:antigen:adjuvant is 1:0.2 :0.1. After vort...

Embodiment 2

[0113] 1.1 Preparation of vaccine

[0114]Ultrasonically disperse nano-calcium carbonate in ultrapure water, the concentration of nano-calcium carbonate is 1mg / mL, and the ultrasonic time is 5min to form nano-calcium carbonate suspension. Polylysine (15000Da) was dissolved in ultrapure water at a concentration of 5 mg / mL to form a polylysine solution. Add the polylysine solution to the nano-calcium carbonate suspension, mix equal volumes, vortex for 15 seconds, and then let stand at room temperature for 15 minutes; then centrifuge and wash at 6000r / min for 10 minutes and wash twice to obtain the carrier particles.

[0115] The carrier was dispersed in ultrapure water at a concentration of 1 mg / mL. After that, add an equal volume of antigen-adjuvant mixture, the antigen is a commercially available model antigen (ovalbumin OVA antigen), the adjuvant is oligonucleotide CpG, and the mass ratio of carrier:antigen:adjuvant is 1:0.2 :0.1. After vortexing for 15 s, let stand at ro...

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Abstract

The invention provides a nano vaccine and a preparation method thereof. The nano vaccine comprises a positively charged carrier particle and a negatively charged wrapper loaded on the surface of the carrier particle; the carrier particle is a nano-calcium carbonate particle with the surface adsorbed with a cationic polymer; the wrapper is an antigen and an adjuvant. Accordingly, the cationic polymer modified nano-calcium carbonate particle serves as an electropositive vaccine carrier to load the negative antigen and the adjuvant, and the endocytosis efficiency of the antigen and the adjuvant can be effectively improved; meanwhile, dendritic cells can be effectively activated, and the in vivo immune response is activated.

Description

technical field [0001] The invention relates to the technical field of immunology, in particular to a nano vaccine and a preparation method thereof. Background technique [0002] Diseases such as tumors seriously threaten the health and development of human beings. Traditional tumor treatment methods include surgery, chemotherapy, and radiotherapy. While causing great pain to patients, they cannot completely cure tumors. Tumor immunotherapy mainly mobilizes the patient's own immune system to fight against tumors, and has become an emerging therapy that is expected to completely cure tumors. [0003] At present, immunotherapy has made great research breakthroughs in a variety of tumor models and other disease models, bringing hope of cure to patients. Tumor vaccines, immune checkpoint blockade therapy represented by PD-1 / PD-L1, and CAR-T therapy have achieved breakthrough results in various tumor clinical trials. Among them, tumor vaccines have attracted the attention of a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/16A61K39/00A61K39/395A61K47/02A61K47/34A61K47/32A61P37/04
CPCA61K9/1611A61K9/1635A61K9/1641A61K9/1676A61K39/00A61K39/395A61K2039/55561A61P37/04
Inventor 田华雨胡莹莹陈杰林琳陈学思
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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