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A kind of magnetic composite nanomaterial and its preparation method and application

A technology of magnetic composite nanoparticles and magnetic nanoparticles, which is applied in the field of nanomaterials and biomedicine, can solve the problems affecting the uptake of nanoparticles by cells, achieve good tissue penetration ability, improve cell endocytosis efficiency, and promote the effect of interaction

Active Publication Date: 2022-05-17
CIXI INST OF BIOMEDICAL ENG NINGBO INST OF MATERIALS TECH & ENG CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is known that polyethylene glycol as a surface modification of nanoparticles can prolong the in vivo circulation time of nanoparticles, but also affects the uptake of nanoparticles by cells

Method used

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  • A kind of magnetic composite nanomaterial and its preparation method and application
  • A kind of magnetic composite nanomaterial and its preparation method and application
  • A kind of magnetic composite nanomaterial and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0117] A preparation method of a pH-responsive magnetic composite nanoparticle diagnosis and treatment reagent comprises:

[0118] Step 1: Dissolve 8g of methoxy PEG (mPEG) and 1.8g of p-formylbenzoic acid (FBA) in 160mL of dichloromethane, add 20mL of dimethylformamide (DMF), and then add 2.48g of dicyclohexyl Carbodiimide (DCC) and 732mg 4-dimethylaminopyridine (DMAP), stirred for 24h.

[0119] Step 2: The system obtained in step 1 was subjected to vacuum filtration for 3 times to remove the precipitate, and the obtained clarified system was purged with nitrogen to remove the solvent, then dispersed in 50 mL of deionized water, and freeze-dried to obtain mPEG-FBA (a polymer with an aldehyde phenyl group at the end). )powder.

[0120] Step 3: Synthesis of superparamagnetic ferric oxide nanoparticles (SPION): get 0.81g ferric chloride hexahydrate (FeCl 3 ·6H 2 O), 0.556g ferrous sulfate heptahydrate (FeSO 4 ·7H 2 O), dissolved in 15mL of deionized water, after the reactio...

Embodiment 2

[0130] Step 1: Dissolve 8g of methoxy PEG (mPEG) and 1.8g of p-formylbenzoic acid (FBA) in 160mL of dichloromethane, add 20mL of dimethylformamide (DMF), and then add 2.48g of dicyclohexyl Carbodiimide (DCC) and 732mg 4-dimethylaminopyridine (DMAP), stirred for 24h.

[0131] Step 2: The system obtained in step 1 was vacuum filtered three times to remove the precipitate, and the obtained clarified system was purged with nitrogen to remove the solvent, then dispersed in 50 mL of deionized water, and freeze-dried to obtain mPEG-FBA powder.

[0132] Step 3: Synthesis of superparamagnetic ferric oxide nanoparticles (SPION): get 0.81g ferric chloride hexahydrate (FeCl 3 ·6H 2 O), 0.556g ferrous sulfate heptahydrate (FeSO 4 ·7H 2 O), dissolved in 15mL of deionized water, after the reaction system was fed with nitrogen for 50min, the temperature was raised to 75°C, and 3mL of ammonia water (the mass concentration was 35% NH 3 ·H 2 0), mixing and stirring 30min, obtains the mixed ...

Embodiment 3

[0142] Step 1: Dissolve 8g of methoxy PEG (mPEG) and 1.8g of p-formylbenzoic acid (FBA) in 160mL of dichloromethane, add 20mL of dimethylformamide (DMF), and then add 2.48g of dicyclohexyl Carbodiimide (DCC) and 732mg 4-dimethylaminopyridine (DMAP), stirred for 24h.

[0143] Step 2: The system obtained in step 1 was vacuum filtered three times to remove the precipitate, and the obtained clarified system was purged with nitrogen to remove the solvent, then dispersed in 50 mL of deionized water, and freeze-dried to obtain mPEG-FBA powder.

[0144] Step 3: Synthesis of superparamagnetic ferric oxide nanoparticles (SPION): get 0.81g ferric chloride hexahydrate (FeCl 3 ·6H 2 O), 0.556g ferrous sulfate heptahydrate (FeSO 4 ·7H 2O), dissolved in 15mL of deionized water, after the reaction system was fed with nitrogen for 50min, the temperature was raised to 75°C, and 3mL of ammonia water (the mass concentration was 35% NH 3 ·H 2 0), mixing and stirring 30min, obtains the mixed l...

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Abstract

The application discloses a magnetic composite nanomaterial and its preparation method and application, including modified magnetic nanoparticles and a modified macromolecular layer located on the outer surface of the modified magnetic nanoparticle, and the modified macromolecular layer includes macromolecular Molecules and polymers linked to the macromolecules via pH responsive groups, the responsive pH value of the pH responsive groups being <6.8. The magnetic composite nanomaterial exists in the form of clusters before reaching the tumor site. Due to its large particle size, it is not easy to be cleared by rapid renal metabolism; after reaching the tumor site, the assembly (magnetic composite nanomaterial) gradually disperses under the slightly acidic condition of the tumor. The tumor site exists in the form of small-sized magnetic nanoparticles, thereby increasing its tumor tissue penetration depth, while the shedding of surface-modified polymers increases the surface positive charge, and the nanoparticles are more easily taken up by cells. Therefore, the pH-responsive magnetic composite nanomaterial cluster provided by the present application not only enhances the contrast effect of MRI imaging, but also improves its tissue penetration.

Description

technical field [0001] The application relates to a magnetic composite nanomaterial and its preparation method and application, belonging to the fields of nanomaterials and biomedicine. Background technique [0002] Cancer is one of the major factors that threaten human life. Cancer Research Institute pointed out that the lack of effective screening and early diagnosis is an important factor for the high number of cancer cases. Early diagnosis is an important factor affecting the prognosis of malignant tumors and a prerequisite for early treatment. [0003] Magnetic resonance imaging (MRI) is a commonly used non-invasive method for early diagnosis of tumors in clinical practice. It has the advantages of high resolution, high tissue penetration, non-invasiveness, no radiation, and low inspection cost. ability. [0004] Superparamagnetic iron oxide NPs are popular in biomedicine because they contain degradable iron elements that can be recycled by cells using biochemical pat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/06A61K49/12A61K49/00A61K41/00A61K47/59A61P35/00
CPCA61K41/0052A61K47/59A61K49/12A61K49/0019A61K49/0002A61P35/00
Inventor 沈折玉钱昆吴爱国
Owner CIXI INST OF BIOMEDICAL ENG NINGBO INST OF MATERIALS TECH & ENG CHINESE ACAD OF SCI
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