A thermosensitive polymersome and its preparation method and application

A temperature-sensitive polymer and polymer technology, applied in drug combination, drug delivery, pharmaceutical formulation, etc., can solve problems such as difficult to control the concentration of gas molecules, unable to target tissues or cells, etc., to improve the curative effect and safety of orthodontics Sex, improve proliferation and differentiation ability, and promote the effect of remodeling

Active Publication Date: 2021-06-04
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the methods of drug delivery are concentrated on direct injection of donor NaHS, sustained release of donor GYY4137, or combined application with anti-inflammatory drugs, all of which have problems such as difficulty in controlling the concentration of gas molecules, inability to target tissues or cells, etc., restricting its function.

Method used

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  • A thermosensitive polymersome and its preparation method and application
  • A thermosensitive polymersome and its preparation method and application
  • A thermosensitive polymersome and its preparation method and application

Examples

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preparation example Construction

[0052] A method for preparing thermosensitive polymersomes, comprising the steps of:

[0053] (1) Synthetic steps of thermosensitive polymer:

[0054] a. Using PEO-DDMAT as a macroinitiator, add N-isopropylacrylamide (NIPAM), coumarin monomer (CMA), and free radical initiator (AIBN) into a round bottom flask, and then add an appropriate amount of The second type of solvent, mix and remove the oxygen in the reaction system, then react at 70 ° C for 24h, and terminate the reaction by exposing to air, the resulting polymer PEO-b-P (NIPAM-stat-CMA) and tert-butyl acrylate (tBA), a free radical initiator (AIBN) and a second type of solvent are mixed to remove oxygen in the reaction system, and then react at 70° C. for 24 hours, and then terminate the reaction by exposing to air to obtain a polymer solution;

[0055] b. The obtained polymer solution was rotary evaporated, dissolved, and precipitated in n-hexane three times, and dried in vacuum at 25°C to obtain a polymer powder;

...

Embodiment 1

[0069] This embodiment provides a method for preparing thermosensitive polymersomes, comprising the following steps:

[0070] (1) Synthesis steps of thermosensitive polymer PEO-b-P(NIPAM-stat-CMA)-b-PAA:

[0071] a. Using 0.68g (0.30mmol) PEO-DDMAT as a macroinitiator, 2.71g (24.00mmol) N-isopropylacrylamide (NIPAM), 1.00g (3.3mmol) coumarin monomer (CMA) , 7.38mg (0.045mmol) free radical initiator (AIBN) was added in the 25mL round bottom flask, then added 3mL of dioxane, until all the reactants were dissolved, blowing argon for 15 minutes by bubbling to remove the reaction Oxygen in the system (forming an oxygen-free environment), carry out reversible addition-fragmentation chain transfer polymerization (RAFT), the reaction temperature is 50 ° C, the reaction time is 48h, the reaction is terminated by exposure to air, and the obtained polymer PEO-b-P (NIPAM-stat-CMA) is mixed with 0.46g (3.6mmol) tert-butyl acrylate (tBA) 7.38mg (0.045mmol) free radical initiator (AIBN) and...

Embodiment 2

[0080] This embodiment provides a method for preparing thermosensitive polymersomes, comprising the following steps:

[0081] (1) Synthesis steps of thermosensitive polymer PEO-b-P(NIPAM-stat-CMA)-b-PAA:

[0082] a. With 0.68g (0.30mmol) PEO-DDMAT as a macroinitiator, 3.39g (30.00mmol) N-isopropylacrylamide (NIPAM), 1.21g (4.0mmol) coumarin monomer (CMA) , 7.38mg (0.045mmol) of free radical initiator (AIBN) was added to a 25mL round bottom flask, and then 5mL of dimethylformamide was added, and until all the reactants were dissolved, argon gas was blown for 15 minutes by bubbling to remove Oxygen in the reaction system (form an oxygen-free environment), carry out reversible addition-fragmentation chain transfer polymerization (RAFT), the reaction temperature is 60 ° C, the reaction time is 36h, the reaction is terminated by exposure to air, and the obtained polymer PEO- b-P (NIPAM-stat-CMA) is mixed with 0.51g (4.0mmol) tert-butyl acrylate (tBA), 7.38mg (0.045mmol) free radic...

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Abstract

The invention relates to a temperature-sensitive polymer vesicle and its preparation method and application. The temperature-sensitive polymer vesicle includes a vesicle body assembled from an amphiphilic temperature-sensitive block-containing polymer, combined in the The biotin-modified Biotin‑CD146 monoclonal antibody and streptavidin (SA), which can target periodontal ligament stem cells (PDLSCs) on the vesicle body, can be used as hydrogen sulfide carriers. Compared with the prior art, the present invention has periodontal ligament stem cells (PDLSCs) targeting, can improve the proliferation and differentiation ability of PDLSCs, promote periodontal tissue reconstruction in an inflammatory environment, and improve the curative effect and safety of orthodontics in an inflammatory state and other advantages, and provide technical support for the clinical application of gas molecules.

Description

technical field [0001] The invention belongs to the field of polymer materials, and in particular relates to a temperature-sensitive polymer vesicle carrying a hydrogen sulfide donor and targeting periodontal ligament stem cells, a preparation method and application thereof. Background technique [0002] It is difficult for patients to maintain good oral hygiene during orthodontic treatment, which often causes periodontal tissue inflammation. If it cannot be controlled in time, it will easily lead to loss of periodontal attachment and rapid alveolar bone resorption. Even after the basic periodontal treatment is completed, the regeneration and reconstruction ability of periodontal tissue will decline, which restricts the safe and effective implementation of orthodontic treatment. During the process of orthodontic periodontal remodeling, periodontal ligament cells (PDLCs), especially the mesenchymal stem cells - periodontal ligament stem cells (PDLSCs), determine the regenerat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/68A61K47/69A61K33/04A61P1/02
CPCA61K33/04A61K47/6898A61K47/6915A61P1/02
Inventor 华咏梅杜建忠廖雨瑶王月倪可人赵逸
Owner TONGJI UNIV
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