The invention provides a preparation method of a digital green intelligent high-purity
marsdenia tenacissima component group and
marsdenia tenacissima
glycoside H and a pharmaceutical composition thereof, and belongs to the technical field of
traditional Chinese medicine preparation. The preparation method comprises the following steps: taking, stirring and extracting
marsdenia tenacissima middlepowder with a 30%
alcohol solution; carrying out throwing
filtration and intelligent cross-flow
filtration; passing through a macroporous adsorption resin column; performing gradient washing with an 8-16BV 0-50%
alcohol solution; eluting with a 3BV 50%-60%
alcohol solution; collecting target
marsdenia tenacissima component group eluent, recovering alcohol until the
alcohol content is 15%, passingthrough a
macroporous resin column for enrichment or
spray drying to obtain
marsdenia tenacissima component groups, or passing through a C18
alkyl bonded
silica gel column, washing and eluting with 46%
acetonitrile solution, collecting target interval eluent, recovering
acetonitrile until the target interval eluent is exhausted, and
spray drying to obtain
marsdenia tenacissima glucoside H. The method has the advantages of simple operation, energy saving, emission reduction, intelligent on-line control of the whole process, stable and controllable product quality, realization of
digitization, greenization and intelligentization, and suitableness for industrial production of traditional Chinese medicines.