Preparation method of digital green intelligent high-purity marsdenia tenacissima component group and marsdenia tenacissima glycoside H and pharmaceutical composition thereof

Abstract

The invention provides a preparation method of a digital green intelligent high-purity marsdenia tenacissima component group and marsdenia tenacissima glycoside H and a pharmaceutical composition thereof, and belongs to the technical field of traditional Chinese medicine preparation. The preparation method comprises the following steps: taking, stirring and extracting marsdenia tenacissima middlepowder with a 30% alcohol solution; carrying out throwing filtration and intelligent cross-flow filtration; passing through a macroporous adsorption resin column; performing gradient washing with an 8-16BV 0-50% alcohol solution; eluting with a 3BV 50%-60% alcohol solution; collecting target marsdenia tenacissima component group eluent, recovering alcohol until the alcohol content is 15%, passingthrough a macroporous resin column for enrichment or spray drying to obtain marsdenia tenacissima component groups, or passing through a C18 alkyl bonded silica gel column, washing and eluting with 46% acetonitrile solution, collecting target interval eluent, recovering acetonitrile until the target interval eluent is exhausted, and spray drying to obtain marsdenia tenacissima glucoside H. The method has the advantages of simple operation, energy saving, emission reduction, intelligent on-line control of the whole process, stable and controllable product quality, realization of digitization, greenization and intelligentization, and suitableness for industrial production of traditional Chinese medicines.

Description

technical field [0001] The invention provides a preparation method of digitized, green, intelligent, high-purity vine vine component group and vine glycoside H, a pharmaceutical composition and its application. Background technique [0002] Tongguan vine is the dry cane of Marsdenia tenacissima (Roxb) Wight et Arn, a plant of the genus Marsdenia tenacissima (Roxb) Wight et Arn, also known as black bone vine, Tongguangsan, Tongtiansan, and Tongguansan. It is widely distributed in tropical and subtropical regions of Asia. The 1977, 2010, and 2015 editions of "The Pharmacopoeia of the People's Republic of China" were recorded under the name "Tengguan Teng". Tong Guan Teng, bitter in taste and slightly sweet, has the effects of anti-inflammatory and pain-relieving, heat-clearing and detoxifying, relieving cough and relieving asthma. Modern pharmacology and clinical trials have proved that Tongguanteng has the functions of anti-cancer, lowering blood pressure, and regulating imm...

AI Technical Summary

Problems solved by technology

Although Tengguan vine contains more than a hundred kinds of active ingredients, the content of each component is very small, and the highest content of Tengguan glycoside H is only about 0.1%, and the water solubility of Tengguan glycosides is small. The preparation process is to use boiled alcohol Shen, the loss of effective parts is relatively large. At present, the content of active ingredients in the extract of Guanguanteng and its preparations is relatively low, and the color is relatively dark. Many, especially the quality of medicinal materials is affected by the place of origin, growth period, harvesting season, processing methods, etc. The quality of the product does not have a digital fixed dose, and it is difficult to control the uniformity of the product within a batch and the reproducibility between batches

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