The invention provides a separation and purification method of
eimeria tenella sporozoite and an
inoculation method, and relates to the technical field of
biology. The separation and purification method comprises the following steps: taking infected
chicken manure, adding water, and filtering; centrifuging, adding a saturated
saline solution into the precipitate, centrifuging, and adding water into the supernate to obtain an oocyst
diluent; centrifuging, and adding
chloramine T solution into precipitate to obtain sporulated oocyst suspension; centrifuging, adding saturated
saline solution into the precipitate for resuspending, centrifuging and collecting oocyst precipitate; adding a
sodium hypochlorite solution for resuspension, and collecting oocysts; and shaking and crushing, stopping shaking when all sporangia escapes, centrifuging to obtain sporangia, adding pancreatin digestive juice for
digestion, filtering, centrifuging, and collecting precipitate to obtain
eimeria tenella sporozoites. According to the
inoculation method, the sporangiums or sporozoites are accurately injected into the middle sections of muscular stomachs and small intestines of the chickens, and successful infection is achieved. The purification degree of the sporangiums and sporozoites obtained by the purification method is high, the experimental operation is simple and convenient, and the infection success rate after injection
inoculation reaches 100%.