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Probiotic microcapsule and preparation method thereof

A probiotic and microcapsule technology, applied in microcapsule preparation, microsphere preparation, bacteria and other directions, can solve the problems of poor survival rate of probiotics, and achieve the effect of improving efficiency, improving survival rate and prolonging digestion time.

Active Publication Date: 2021-08-06
郑州伊品医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commonly used probiotic microencapsulation methods include spray drying, emulsification, extrusion and gel technology, etc. However, these methods still have the problem of poor survival rate of probiotics

Method used

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  • Probiotic microcapsule and preparation method thereof
  • Probiotic microcapsule and preparation method thereof
  • Probiotic microcapsule and preparation method thereof

Examples

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Comparison scheme
Effect test

preparation example Construction

[0040] An embodiment of the present invention proposes a method of preparing a probiotic microcapsule, comprising the steps of:

[0041] 1) Add phosphate buffer to porous starch, stand, add polyethylene imine, constant temperature oscillation, washing, drying, porous starch-polyethyleneimine carrier;

[0042] 2) Mix the above porous starch-polyethylene mine support and solid grease, a solution of an emulsifier, 40 ° C ~ 50 ° C constant temperature emulsification, cooling, porous starch-polyethyleneimine carrier of the solid grease;

[0043] 3) In the solution of the porous starch-polyethyleneimine carrier of the above-mentioned solid oil, triethylamine, dimethyl sulfoxide, stirring, and acetic anhydride, reaction, then dialyzed, freeze and dry, gave acetylated Porous starch-polyethyleneimine vector;

[0044] 4) In the above acetylated porous starch-polyethylene imine, the solution of the emulsifier is added, 40 ° C ~ 50 ° C emulsified, adding a lipase, 40 to 50 ° C for 10 to 20 mi...

Embodiment 1

[0091] Example 1 Preparation method of probiotics microcapsules, including the following steps:

[0092] (1) Preparation of porous starch: 2 g of strain is added to 250 mL of triangular bottle, adding 0.2 mol / L of sodium hydrogen phosphate and 0.1 mol / L citric acid buffer, 40mL, add 0.1 ml of toluene, add 0.1 ml of toluene to add appropriate dilution The enzyme solution, 40 ° C, constant temperature oscillated 24 h, centrifugal separation supernatant, precipitate at 60 ° C for normal pressure drying, and pulverized.

[0093] (2) Porous starch-polyethyleneimine carrier preparation: accurately weigh 2.0 g of corn porous starch, placed in a beaker, to balance the vector, add 200 ml of phosphate buffer (pH 8.0), put 1 h, then Among them, 1.0 g of polyethylene imine was added, 40 ° C constant temperature was oscillated for 10 h, and after the reaction was completed, washed with pH 8.0 by the buffer until the filtrate was not tetrabenzenesulfonic acid reaction (TNBS). Wash with dis...

Embodiment 2

[0105] Example 2 Preparation method of probiotics microcapsules, including the following steps:

[0106] In the same embodiment, the difference is that step (12): repeating the above two steps until the 4-layer layer layer self-assembled probiotic microcapsules are assembled.

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Abstract

The invention provides a probiotic microcapsule and a preparation method thereof, and belongs to the technical field of microcapsules. The preparation method comprises the following steps: respectively preparing a porous starch-polyethyleneimine carrier, a porous starch-polyethyleneimine carrier filled with solid grease, an acetylated porous starch-polyethyleneimine carrier, a porous starch-polyethyleneimine carrier without solid grease, and porous starch bacterial sludge adsorbing probiotics; and embedding the porous starch bacterial sludge adsorbing the probiotics by adopting biological polyelectrolyte to obtain the layer-by-layer self-assembled probiotic microcapsules. According to the probiotic microcapsule disclosed by the invention, survival rate of the probiotics can be remarkably improved.

Description

Technical field [0001] The present invention belongs to the technical field of microcapsules, and more particularly to a probiotic microcapsule and a preparation method thereof. Background technique [0002] Probiotics is a kind of active microorganisms that have beneficial human body, have the effect of adjusting the harvest of the accommodation, promoting the effect of intestinal nutrient absorption, and has been committed to combining probiotics with food to prepare the functionality of probiotics. Food, health food, etc. For example, probiotics are added to yogurt, milk beverage, milk powder, bread and biscuits and other articles, and prepared bacteria powder, capsules, etc. containing probiotics. However, some unfavorable factors (such as temperature, processing conditions, gastric acid, biliary salt, etc.) will cause loss to probiotics, thereby reducing their health efficacy in the host. [0003] In order to ensure a sufficient amount of live bacteria, the minimum amount of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/135A23L29/30A23L29/00A23L29/10A23P10/30C12N1/20
CPCA23L33/135A23L29/30A23L29/045A23L29/035A23L29/04A23L29/10A23P10/30C12N1/20A23V2002/00B01J13/02A23V2200/3204A23V2250/5118A23V2250/1618A23V2250/1614A23V2250/032A23V2250/18A23V2250/511A23V2250/5072Y02A50/30
Inventor 刘欢王倩玉姚浩东胡婕伦聂少平谢明勇
Owner 郑州伊品医药科技有限公司
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