A kind of forensic medicine diatom test method

Abstract

The invention relates to the field of forensic medical inspection, and relates to a forensic medical diatom inspection method. It at least includes the following steps: (1) Microwave digestion: add concentrated nitric acid and hydrogen peroxide solution to the test sample and then carry out microwave digestion; (2) Vacuum suction filtration: carry out vacuum suction filtration on the digestion solution, and then Add ultra-pure water to continue the suction filtration to make the surface of the filter membrane close to neutral, and finally add absolute ethanol to remove the water in the filter membrane by suction filtration; (3) Scanning electron microscope automatically takes pictures and stores the images (5) Qualitative and quantitative analysis of diatoms: using manual identification Check, classify and statistically process the diatoms in the captured field of view images by means of automatic recognition by computer. The invention has high detection sensitivity, can effectively avoid pollution, is simple, efficient, environmentally friendly, and has accurate qualitative and quantitative analysis, greatly improves the working environment of forensic diatom inspection technicians, reduces labor intensity, and has good application in the practice of forensic drowning diagnosis prospect.

Description

technical field [0001] The invention relates to the field of forensic medical inspection, in particular to a forensic medical diatom inspection method for identifying the cause of death of corpses in water by inspecting the diatom species and content in organs, tissues and organs. Background technique [0002] The cause of death analysis of corpses in water has always been one of the difficulties in the practice of forensic medicine. Since the 1940s, many forensic scientists have confirmed on the bodies of the drowned that diatoms can enter various organs of the large circulation. Although there are still differences in the diagnostic value of diatom detection in theory, in forensic practice, diatom detection is still considered to be a relatively reliable means of diagnosing drowning, especially for the identification of the cause of death of decayed corpses in water. good identification method. Under the condition that pollution can be prevented, more diatoms of the same...

AI Technical Summary

Problems solved by technology

[0004] The traditional strong acid digestion method is open. During the digestion process, there is a danger of strong acid spraying out and burning the human body. In addition, a large amount of strong acid and corrupted test materials in forensic practice release nitrogen dioxide and malodorous gas during the digestion process, which not only pollutes the environment Moreover, the working environment of the forensic examiners is abnormally bad; and the traditional strong acid digestion method generally takes repeated centrifugation and then makes a film for observation. The repeated centrifugation process will lead to the loss of diatoms. Experiments show that centrifugation once leads to about 10% Diatom loss, increased centrifugation speed does not significantly improve diatom recovery, thus resulting in low recovery of diatom extraction from visceral tissue

[0005] At present, most forensic laboratories only use optical microscopes for qualitative and quantitative analysis of diatoms in digested tissues. However, optical microscope analysis is intensive and limited by the magnification. For micro diatoms, it is easy to miss or cannot accurately identify them species

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