59results about How to "High binding capacity" patented technology

The present invention relates to the production of texturized microcrystalline cellulose from raw pulp material. This texturized microcrystalline cellulose can then be used for surface treatment of paper or paper board. Additionally, the texturized microcrystalline cellulose may be used as a starting material for production of paper or paper board.

The invention relates to a preparation method and application of a magnetic silicon dioxide composite microsphere. Superparamagnetic ferroferric oxide nano particles of which the diameter ranges from 4 nm to 30 nm are prepared through a high-temperature pyrolysis method, a silicon dioxide shell of which the thickness ranges from 5 nm to 20nm covers the outer surfaces of the magnetic ferroferric oxide nano particles through a reverse microemulsion method, amination modification is conducted on the silicon dioxide surface, glutaraldehyde is used as a crosslinking arm, ligand protein is connected into, and protein separation is conducted through specific binding of ligand protein and target protein. The prepared magnetic microsphere is small in particle diameter and good in monodispersity, the composite microsphere with amine is large in specific surface area, nucleophilic addition is utilized, after the crosslinking arm glutaraldehyde is connected into, multiple kinds of ligand protein can be connected into, and then multiple kinds of target protein can be separated. The method is suitable for rapid separation and application of protein in biological samples, and has wide application prospect and great application value in the biomedical field and other fields.

The present invention provides a process for preparing a functionalised polymeric chromatography medium, which process comprises (I) providing two or more non-woven sheets stacked one on top of the other, each said sheet comprising one or more polymer nanofibres, (II) simultaneously heating and pressing the stack of sheets to fuse points of contact between the nanofibres of adjacent sheets, and (III) contacting the pressed and heated product with a reagent which functionalises the product of step (II) as a chromatography medium.

Magnetic particles capable of binding a target substance, which comprise a magnetic material and a matrix material, wherein the magnetic material is remanent upon exposure to a magnetic field and the matrix material has a surface comprising functional groups which promote disaggregation of the particles in the presence of a liquid phase.

The present invention relates to a method of separating one or more immunoglobulin containing proteins from a liquid. The method includes first contacting the liquid with a separation matrix comprising ligands immobilised to a support; allowing the immunoglobulin containing proteins to adsorb to the matrix by interaction with the ligands; followed by an optional step of washing the adsorbed immunoglobulin containing proteins; and recovering said immunoglobulin containing proteins by contacting the matrix with an eluent which releases the proteins. The method improves upon previous separation methods being that each of the ligands consists essentially of a monomer or dimer SpA or protein Z or a functional variant thereof.

The invention discloses a preparation method and application of metal chelating agarose gel, and belongs to the field of protein chemistry. The method for preparing the metal chelating agarose gel comprises the steps of activating and coupling. An agarose chelating medium with 6 ligands is formed by connecting ethylene diamino-disuccinic acid to sepharose. The metal chelating agarose gel can be applied to the removal of metal ions such as Cu, Zn, Mn, Cd and the like in a protein solution, and has high binding ability and binding capacity, and the removal rate reaches 93 percent. The preparation process is simple, easy to scale up and convenient to use; and the metal chelating agarose gel can be better applied to the removal of the heavy metal ions in the research of plant proteins which grow in a complex heavy metal polluted environment, and can also be applied to other protein scientific research in which metal ions are required to be removed.

The invention discloses a polycrystalline diamond composite tooth, a preparation method and a down-hole drill bit. The polycrystalline diamond composite tooth comprises a hard alloy matrix and a polycrystalline diamond layer, wherein an impact-resistant layer is arranged between the hard alloy matrix and the polycrystalline diamond layer, and consists of the following components in percentage by weight: 75-80 percent of diamond, 10-15 percent of WC (Wolfram Carbide) and the balance of adhesive. According to the polycrystalline diamond composite tooth, the impact-resistant layer is arranged between the hard alloy matrix and the polycrystalline diamond layer, and consists of the diamond, the WC and the adhesive, so that the binding force between the hard alloy matrix and the polycrystalline diamond layer is improved by the impact-resistant layer, tensile stress concentration is effectively reduced, the impact toughness of the polycrystalline diamond composite tooth is improved, further the quality of the polycrystalline diamond composite tooth is improved, and the service life is prolonged.

To provide a novel packing material for liquid chromatography capable of separating and purifying, or collecting and recovering, a biopolymer such as a protein or a peptide by adsorption and desorption by a pH change without being influenced by the isoelectric point of the protein or by the salt concentration in a solvent in which the biopolymer such as the protein is dissolved, and to provide a process for concentrating and recovering a desired biopolymer such as a protein or a peptide from a large amount of dilute cell culture solution by means of such a packing material. Separation and purification, or collection and recovery, of a biopolymer, is carried out by liquid chromatography by means of a packing material for liquid chromatography comprising a base matrix and a ligand immobilized to the base matrix, wherein the base matrix is a hydrophilic base matrix having alcoholic hydroxy groups on its surface, the ligand is at least one ligand selected from the group consisting of an +--amino acid represented by the following formula (1): RCH(NH 2 )COOH (1) wherein R is an aromatic group or a C 5-7 non-ionic aliphatic group, and an aminomethyl benzoic acid, and the ligand is immobilized to the base matrix by an amide bond or an urethane bond via the amino group contained in the compound represented by the formula (1).

The present invention relates to a granulated seasoning salt and a preparation method thereof, characterized by using a gelatinized tapioca starch solution as a binder and an anti-caking agent. The granulated seasoning salt according to the present invention is prepared by using a gelatinized tapioca starch solution as a binder and an anti-caking agent to solve the problem such as caking property due to moisture absorption. Thus, the prepared seasoning salt has high binding capacity and stability, does not turn brown, is rapidly eluted, and can be stored for a long period of time without dissociation of the mixed materials.

A process for the production of a reaction chamber assembly, wherein a flat substrate and bottomless reaction chambers are provided, the substrate is first loaded with a biological agent and then the bottomless reaction chambers are bonded glue-free to the substrate, in particular through laser bonding, and liquid-tight reaction chambers, for instance individual wells, individually connected wells, such as strips, or wells in the form of a microtiter plate, are obtained. The present invention further provides a kit comprising a substrate suitable for being loaded with at least one biological agent and at least one bottomless reaction chamber, wherein the kit is suitable for glue-free bonding of the bottomless reaction chamber to the substrate.

The invention discloses a preparation method of a new material for separating chiral aspartic acid, and solves the problems of difficulty and low efficiency of L-Asp separation currently. The method comprises the following steps of: activation of silica gel particles; chemical bonding of the silica gel particles with a double bond-containing silane coupling agent; chemical grafting of the silica gel particles with polydimethylaminoethyl methacrylate; and preparation of silica gel particle surface molecularly imprinted polymer. The invention adopts silica gel particles as carriers, adopts dimethylaminoethyl methacrylate as a functional macromolecular material grafted on the particles or the particle surfaces, and adopts ether dihalide as a cross-linking agent to prepare the surface imprinted material with high molecular binding speed, strong recognition selectivity, high binding capacity to L-Asp; the surface imprinted material prepared by the invention is suitable for the requirements of industrial production, and has high material utilization rate, low cost, and very good selectivity, recognition and bonding performance to L-Asp molecules.

The invention provides a sensor chip for detecting microcystic toxins and mobile monitoring equipment thereof. The sensor chip comprises a combined chip body, the upper surface of the combined chip body is modified with a microcystic toxin molecularly-imprinted membrane, and the microcystic toxin molecularly-imprinted membrane is provided with micropores for specific recognition of microcystic toxins. The mobile monitoring equipment comprises an optical system with the sensor chip, and a flow path system. The invention further provides a device for detecting the content of microcystic toxins in a water area in real time. The content of microcystic toxins in the water area can be detected in real time in situ, and the instrument can operate automatically in the field without artificial participation.

A process for large scale separation of molecules comprising the steps of providing magnetic porous particles having an affinity to said molecules to be separated; mixing said magnetic porous particles with a solution containing said molecules; bringing said mixture in contact with a magnetic separation device comprising a flow channel and at least one magnetic element; removing said at least one magnetic element and collecting the magnetic porous particles carrying said molecules; separating said molecules from said magnetic porous particles; obtaining a concentrated fraction of said molecules; and recirculating the magnetic porous particles .A system for performing this process.

The invention discloses an improved magnetic silicon particle. The preparation method of the improved magnetic silicon particle comprises the following steps: 1) preparing two-dimensional titanium dioxide nano-sheet; 2) prepare a two-dimensional magnetic titanium dioxide sheet; 3) prepare improved silicon-coated two-dimensional magnetic titanium dioxide wafer. The improved magnetic silicon particles of the invention have high binding capacity to nucleic acids and little adsorption to salt ions, proteins or other impurities, and can effectively solve the phenomenon of virtual high nucleic acidextraction concentration. A method for improve magnetic silicon particles for nucleic acid purification include such steps as using improved magnetic silicon particles as magnetic particle for nucleicacid purification, preparing nucleic acid purification kit with lysis solution, binding solution, cleaning solution and eluent, and extracting high-purity and high-yield nucleic acid from biologicalsample through that kit.

A depth filtration device for the clarification of biological fluids including a composite depth filter media having a nonwoven first layer integral with a second layer containing a polyaeryionitrile (PAN) fibers, a filter aid, and a wet-strength resin. The depth filter media exhibits increased creased binding capacity for soluble impurities such as DNA and host cells proteins from biological / cell culture feed-streams during secondary clarification and low-level impurity clearance of harvested cell culture fluids, such as those used for the manufacture of monoclonal antibodies. The depth filter media additionally exhibits significantly lower flushing requirements, resulting in lower levels of organic, inorganic and bioburden extractables released, high dirt holding capacities and good chemical and / or radiation resistance.

An amicable element / link amicable element magnetic compound particle and its Process for preparing and usage are disclosed. The product contains the assembled gold-magnet compound particle in inner layer and the amicable element / link amicable element biomolecule which is coating in outer layer. The assembled gold-magnet compound particle is balanced with balancing brine solution, the high bioepiderm combined with the active amicable element / link amicable element biomolecule is oscillated and mixed at constant temperature, reacted, magnetic separated and cleaned. The invention resolves the technical matters of poor versatility, few marks, deactivation and denaturation of biomolecule in background technique. The invention can be used in marking the biotin biomolecule and the biotin non-biological material directly and then enriching, separating, scavenging and detecting of the biomolecule and non-biomolecule. The product has the property of biological activity, and can improve the sensitivity and specificity of the bioanalysis and detection. The process for preparing is simple, and save a good deal of precious biological agents. The measuring process is easier.

The invention belongs to the technical field of molecularly imprinted polymers, and particularly relates to a preparation method of protein affinity blotting aquagel polymers based on acrylamide groupmetal chelation monomers. The invention aims at providing the preparation method of the protein affinity blotting aquagel polymers based on the acrylamide group metal chelation monomers by aiming atthe technical analysis and the existing problems. The preparation method of the protein affinity blotting aquagel polymers based on the acrylamide group metal chelation monomers has the technical scheme that according to the method, after the acrylamide group monomers are chelated with divalent metal ions, target protein is subjected to specific adsorption combination and removal; then, polymers are obtained. The preparation method has the following characteristics that the acrylamide group monomers have the metal chelation capability; the molecular mark aquagel has the temperature and pH duplex sensitivity; the reverse phase suspension technology is used; the polymers are aquagel polymers.

The invention discloses a high-carrying-capacity and large-pore-diameter polymer cation exchange chromatography medium and preparation thereof and belongs to the technical field of preparation and application of polymer chromatography media. The cation exchange chromatography medium takes a polyacrylate or polystyrene microsphere as a base material and the surface of the microsphere is grafted with a vinyl functional monomer. A preparation method of the high-carrying-capacity and large-pore-diameter polymer cation exchange chromatography medium comprises the following steps: carrying out swelling treatment on the polyacrylate or polystyrene microsphere in a vinyl functional monomer solution; adding an atom transfer free radical polymerization catalyst ligand system into the microsphere subjected to the swelling treatment and carrying out grafting polymerization reaction; after the polymerization reaction is finished, removing an unreacted monomer and drying in vacuum to obtain the cation exchange chromatography medium. The prepared cation exchange chromatography medium has the advantages of high ion exchange capacity, high protein binding carrying capacity and rapid operation flow,and is suitable for rapidly separating and purifying biological macromolecules.

The invention provides a surface polylysine modifying method for a PDMS micro-fluidic chip.The PDMS micro-fluidic chip is subjected to plasma processing and silanization processing, and then coupled with polylysine, polylysine is firmly bound to the surface of the chip in a covalent binding mode, and the micro-fluidic chip capable of efficiently fixing protein is formed.According to the modified chip, the common advantages of being high in signal-to-noise ratio, high in binding capacity, low in inter-point variation coefficient and the like of a polylysine modifying method are kept, and more importantly, the problem that polylysine and the chip are bound infirmly in a physical absorption mode, and consequently polylysine is prone to fall off especially in microfluidics application is solved.

The present invention concerns a method for producing microparticles loaded with proteins which is characterized in that the microparticles are loaded in suspension under strongly alkaline conditions. The invention also concerns microparticles which can be produced using this method and their use in a binding test e.g. in an immunoassay.

The invention relates to a preparation method of an artificial antibody for detecting heavy metal ions. The surface of a nano SiO2 sphere in the synthesized artificial antibody is coated with an acrylamide and ethylene glycol dimethacrylate polymer shell layer to form a core-shell structure, and the method is characterized in that an imprinting shell layer recognition site is modified with a fluorescent probe; recognition sites in the heavy metal ion artificial antibody shell are eluted to form a cavity structure matched with an imprinted heavy metal ion space structure, the heavy metal ions reenter the recognition sites, and electrons on a recognition site fluorescent probe are captured by utilizing a fluorescence resonance energy transfer mechanism; and high-selectivity recognition and high-sensitivity detection on heavy metal ions are realized. According to the invention, the nano synthesis technology, the molecular imprinting technology and the material surface post-functional modification technology are combined, the key scientific problem that a traditional molecular imprinting material has no signal output is solved, and the method is simple, good in selectivity, high in sensitivity and broad in spectrum for the heavy metal ions.

Process for obtaining human albumin of high efficiency for use in detoxification therapy. The process comprises a) a first dialysis (diafiltration); b) the stabilisation of the solution with NaCl and at least one amino acid; c) heating the solution; d) a second dialysis (diafiltration).

The invention discloses a high-affinity tree-like boric acid functionalized magnetic microsphere as well as a preparation method and an application thereof. The surface of the microsphere contains high-density boric acid radicals, and the polyvalent cooperative binding can be generated for cis-dihydroxy enriched biological molecules, so that bonding force is obviously improved. The dissociation constant of the microsphere to glycoprotein is 10<-5> to 10<-6>M, the bonding force is improved by 3-4 orders of magnitude compared with the ordinary boric acid functionalized material, and the glycoprotein with the concentration of 2*10<-14>M can be enriched. Moreover, the microsphere further has the advantages of being high in anti-jamming capability, high in binding capacity, quick in binding / analyzing speed, and the like. Since the microsphere has magnetism, a sample can be conveniently processed by applying an external field. According to the high-affinity tree-like boric acid functionalized magnetic microsphere disclosed by the invention, the cis-dihydroxy enriched biological molecules with low abundance in a complicated sample can be conveniently and quickly enriched, purified and separated.

The invention discloses water storage porous silicon dioxide magnetic particles as well as a preparation process and application thereof. The water storage porous silicon dioxide magnetic particles comprise the following raw materials: a magnetic core, silicone, silica sol, a pH adjusting substance and a polylactic acid-glycolic acid copolymer. In the reaction composition, the pH regulator can continuously deposit silicone and silica sol on the surface of the magnetic core, and the polylactic acid-glycolic acid copolymer adsorbs the silica sol and causes a silicon dioxide coating to form a reticular porous structure, thereby forming magnetic particles with a diameter of 10[mu]m or less. The silicon magnetic particles provided by the invention have a higher binding capacity to nucleic acidsand less adsorption to salt ions, proteins or other impurities, have specific porosity, and the binding capacity and binding specificity can be obtained by selectively changing parameters of a synthesis reaction.

A particles comprise non-magnetic porous particles, comprising at least one polymer, and having an exterior surface and an interior surface defined by said pores. The non-magnetic porous particle has functional groups on its inner and outer surface. A magnetic particle, which also has functional groups bonded to its surface, is covalently bonded to the non-magnetic porous particles by reacting the functional groups of the two particles and forming a covalent bond between them. The smallest average diameter of the magnetic particle is greater than the average diameter of the pores of the porous particle. In this way, the magnetic particle does not block the pores of the non-magnetic porous particle. The application also concerns a novel system and an assay for the analysis of molecules and cells with the magnetic particle. The magnetic particles on the non-magnetic particles form a characteristic pattern, fingerprint or bar code.

The invention belongs to the fields of material science and engineering and environmental science, and specifically relates to a temperature-sensitive bisphenol A imprinted polymer microsphere and an application thereof. The microspheres use polystyrene as a carrier, bisphenol A (BPA) as a template molecule, combined with temperature-sensitive material monomers, and polymerize to obtain temperature-responsive BPA imprinted polymer microspheres. The temperature-sensitive bisphenol A imprinted polymer microsphere is used for the detection of bisphenol A. The present invention has clear principle, novel design, low cost and simple operation, adopts methods such as dispersion polymerization, swelling polymerization and precipitation polymerization, and the obtained imprinted polymer microspheres have regular morphology and stable properties, and the adsorption rate of bisphenol A is fast and binding It has high capacity, good specificity and good reproducibility. When applied to the detection of seawater / yogurt samples containing bisphenol A, it can achieve a recovery rate of more than 90% and has good reusability. In addition, the smart imprinted polymer obtained in the present invention is sensitive to external temperature, and different solution temperatures have a great influence on its adsorption capacity.

The invention discloses a synthetic method of a potentiometric pesticide sensor, and belongs to a molecular imprinting technology. The synthetic method comprises the following steps: (1) dissolving chloromethylated polysulfone (CMPSF) into dichloromethane, adding dimethylacetamide TDMAC, pouring into a glass culture dish, performing film casting, and immersing into ethidene diamine for amination to prepare an ammoniated polysulfone (AMPSF) membrane with amino on the surface; (2) dissolving absolute ethyl alcohol, sodium styrenesulfonate (SSS) and pirimicarb into water, and adding the AMPSF membrane, an initiator, namely ammonium persulfate and a crosslinking agent, namely bisacrylamide MBA to obtain a pirimicarb molecule-imprinted membrane MIM; (3) establishing the sensor. The grafting-type imprinted membrane has specific recognition selectivity and excellent binding affinity for pirimicarb template molecules, and has the binding capacity as high as 92 microgram / cm<2>; the potentiometric sensor established by using the pirimicarb molecule-imprinted membrane as a sensitive membrane is simple and quick in establishment process and sensitive and accurate in detection.

The invention discloses a controllable and universal directional surface printing method. The method comprises the steps as follows: template glucoprotein is anchored on the surface of a substitution boric acid functional substrate material via boron affinity interaction, the polymer formed by auto-polymerization in water phase of a compound capable of automatically polymerizing forms a printing layer, and a template is eluted under the acid condition to form a printing hollow cavity, as a result, the molecular printing polymer is obtained. The molecular printing polymer prepared via the method not only has the advantages of good specificity, high combination capacity, strong anti-interference capability, quick combination and analysis speed and the like, but also can exclusively identify the template glucoprotein with or without the boron affinity interaction, is adjustable in force of combination with the template when keeping the specificity, and is wide in application range. The method has the advantages of convenience, controllability, universality, high printing efficiency and the like. The molecular printing polymer can exclusively identify, gather, separate and detect the glucoprotein with low enrichment in a complex practical sample.

The invention discloses a preparation method of a new material for separating and purifying chiral tetramisole (TM), which is used for solving the problem of low efficiency of the existing surface imprinted material for separating L-TM. The preparation method comprises the following steps: activating silica gel particles, and connecting the surfaces of the silica gel particles with an amino-containing silane coupling agent through chemical bonds; chemically grafting the surfaces of the silica gel particles with polymethylacrylic acid; and preparing a silica gel particle surface molecular imprinted polymer (SMIP). In the method, the silica gel particles are taken as a carrier, methacrylic acid is taken as a functional macromolecular material grafted with the surfaces of the particles or microspheres, and the amino-containing silane coupling agent is taken as a cross-linking agent to obtain the surface imprinted material which has the advantages of fast binding speed, strong recognitionand selectivity and high binding capacity on L-TM molecules. The surface imprinted material prepared by the method has the advantages of high material utilization ratio, low cost, and good selectivity and recognition and associativity on the L-TM molecules, thus meeting the requirements for industrial production.

The invention relates to an epoxy amino chromatography medium and a preparation method thereof. The epoxy amino chromatography medium is formed by ring-opening reaction of a polysaccharide microsphereand an amino epoxy compound. The preparation method comprises reaction of the polysaccharide microsphere and the amino epoxy compound. The epoxy amino chromatography medium has a long intermediate arm, high protein binding capacity, and simple preparation method.