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Epoxy amino chromatography medium and preparation method thereof

A technology of epoxy ammonia and base layer, which is applied in the field of separation and purification, can solve the problems of long preparation cycle and high production cost, and achieve the effects of low cost, short production cycle and short sample retention time

Inactive Publication Date: 2019-01-15
WUHAN HUIYAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional preparation method of methylamino chromatography medium is to connect the intermediate arm through the activation of the hydroxyl group and the epoxy group on the microsphere, and then the methylamino group is bonded to the microsphere through the amino group and the intermediate arm. The reaction is divided into multiple steps, and the production cost is high. , long preparation cycle

Method used

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  • Epoxy amino chromatography medium and preparation method thereof
  • Epoxy amino chromatography medium and preparation method thereof
  • Epoxy amino chromatography medium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Weigh 1000g of Focusose 6BB microspheres into a 5000mL glass reaction bottle, weigh 200g of pure water into the reaction bottle, stir at a constant speed of 500rpm, weigh 200g of glycidyltrimethylammonium chloride into the glass reaction bottle, and then weigh Take 10g of sodium hydroxide and 10g of sodium borohydride into the reaction flask, heat to 50°C, stir vigorously at a constant speed of 500rpm for 18h to obtain the mixture, remove the supernatant to retain the precipitate, and vacuum filter and wash the microspheres with 10 times the volume of pure water, add Store in 20% ethanol preservation solution at room temperature, and take samples to detect ion load and protein load.

Embodiment 2

[0028] Weigh 1000g of Focusose 6BB microspheres into a 5000mL glass reaction bottle, weigh 200g of pure water into the reaction bottle, stir at a constant speed of 500rpm, weigh 400g of glycidyltrimethylammonium chloride into the glass reaction bottle, and then weigh Add 50g of sodium hydroxide and 10g of sodium borohydride into the reaction flask, heat to 50°C, and stir vigorously at a constant speed of 500rpm for 18 hours to obtain the mixture, remove the supernatant to retain the precipitate, and vacuum filter and wash the microspheres with 10 times the volume of pure water, add Store in 20% ethanol preservation solution at room temperature, and take samples to detect ion load and protein load.

Embodiment 3

[0030] Weigh 1000g of Focusose 6BB microspheres into a 5000mL glass reaction bottle, weigh 200g of pure water into the reaction bottle, stir at a constant speed of 500rpm, weigh 600g of glycidyltrimethylammonium chloride into the glass reaction bottle, and then weigh Add 100g of sodium hydroxide and 10g of sodium borohydride into the reaction flask, heat to 50°C, stir vigorously at a constant speed of 500rpm for 18 hours to obtain the mixture, remove the supernatant to retain the precipitate, and vacuum filter and wash the microspheres with 10 times the volume of pure water, add Store in 20% ethanol preservation solution at room temperature, and take samples to detect ion load and protein load.

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Abstract

The invention relates to an epoxy amino chromatography medium and a preparation method thereof. The epoxy amino chromatography medium is formed by ring-opening reaction of a polysaccharide microsphereand an amino epoxy compound. The preparation method comprises reaction of the polysaccharide microsphere and the amino epoxy compound. The epoxy amino chromatography medium has a long intermediate arm, high protein binding capacity, and simple preparation method.

Description

technical field [0001] The invention relates to the technical field of separation and purification, in particular to an epoxy amino chromatography medium and a preparation method thereof. Background technique [0002] Methylamino chromatography medium is a strong anionic chromatography medium, through the adsorption and combination of positively charged amino groups and negatively charged proteins, viruses and other biomolecules, positively charged impurities flow through, so as to achieve protein or virus separation and purification Purpose. Strong anion chromatography media are often used for the separation and purification of charged biomolecules such as proteins, viruses, polypeptides, and nucleic acids, and are widely used. [0003] The traditional preparation method of methylamino chromatography medium is to connect the intermediate arm through the activation of the hydroxyl group and the epoxy group on the microsphere, and then the methylamino group is bonded to the ...

Claims

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Application Information

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IPC IPC(8): C08B37/12C08B37/02C08B11/145C08B11/14B01J20/286
CPCB01J20/286C08B11/14C08B11/145C08B37/0006C08B37/0039
Inventor 易国军汤志敏聂康乐陈冬景肖胜杰岳平
Owner WUHAN HUIYAN BIOTECH
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