Improved magnetic silicon particles and method for nucleic acid purification thereof

Abstract

The invention discloses an improved magnetic silicon particle. The preparation method of the improved magnetic silicon particle comprises the following steps: 1) preparing two-dimensional titanium dioxide nano-sheet; 2) prepare a two-dimensional magnetic titanium dioxide sheet; 3) prepare improved silicon-coated two-dimensional magnetic titanium dioxide wafer. The improved magnetic silicon particles of the invention have high binding capacity to nucleic acids and little adsorption to salt ions, proteins or other impurities, and can effectively solve the phenomenon of virtual high nucleic acidextraction concentration. A method for improve magnetic silicon particles for nucleic acid purification include such steps as using improved magnetic silicon particles as magnetic particle for nucleicacid purification, preparing nucleic acid purification kit with lysis solution, binding solution, cleaning solution and eluent, and extracting high-purity and high-yield nucleic acid from biologicalsample through that kit.

Description

technical field [0001] The invention relates to the field of bionano materials, in particular to an improved magnetic silicon particle and a method for purifying nucleic acid. Background technique [0002] Conventional methods for extracting nucleic acids from biological samples involve guanidinium isothiocyanate-phenol-chloroform extraction, alcohol precipitation and column separation techniques. However, these processes are time-consuming and difficult to automate. Since Vogelstein et al. reported that glass powder was used as an adsorbent for extracting DNA fragments from agarose gels in the presence of high concentrations of sodium iodide, solid-phase nucleic acid purification techniques based on silica gel and other carriers with hydrophilic surfaces have been widely used. The nucleic acid purification method based on magnetic particles is one of them. Nucleic acid purification using magnetic separation technology usually goes through three steps: adsorption, washing,...

AI Technical Summary

Problems solved by technology

This puts forward higher requirements for magnetic bead nucleic acid extraction, and there are still some downstream applications such as whole exome sequencing, gene chip, Southern blotting technology, fluorescent in situ hybridization technology, etc. still using columns Nucleic acid extraction by the column method is because the quality of the nucleic acid extracted by the column method is relatively stable, 260 / 230 is above 1.8, and 260 / 280 is in the range of 1.7 to 1.9. A problem with the magnetic bead method is that the ultraviolet spectrophotometer measures The concentration is quite different from the gel map or Qubit quantitative concentration, which has a great impact on subsequent library construction.

This is because the specific surface area of ​​the magnetic bead method is larger than that of the column method, and the particle size is too small so that its ability to adsorb impurities is also improved. Nano-scale magnetic beads are very easy to adsorb impurities that can also peak at 260, making it from the ultraviolet The concentration of nucleic acid tested by the spectrophotometer is very high, but the actual quantification by gel map or Qubit will be 2 to 10 times different, that is, "falsely high"

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