The invention relates to an extraction process for industrial mass production of spinosad. The process includes the steps of: fermentation broth pretreatment, frame and plate filtration, and flash drying; mycelium soaking in methanol; concentration of extract liquid, phase inversion washing; back extraction with tartaric acid; adjustment of pH and centrifugation to obtain a spinosad crude product; and recrystallization with methanol to obtain a spinosad fine product. The process provided by the invention extracts spinosad by means of mycelium separation, drying and extraction, and has the characteristics of high extraction efficiency, small solvent dosage, which is only half of that of fermentation broth direct solvent extraction, relatively low energy consumption, and shortened production cycle. In addition, the equipment needed by frame and plate filtration, flash drying, extraction, concentration, washing, back extraction and refining is general, therefore industrial production can be realized easily. The obtained product has high yield up to 85%-90%, after purification of spinosad coarse crystals by solvent recrystallization, the quality of spinosad is improved, and the product content reaches 95%-98%.