The invention discloses an experimental device and method for on-line detection of tire temperature. The device includes a fixture (13), an integrated electronic component (12), a thermocouple, and the temperature detection signal lead-out end (6) of the thermocouple is connected to the integrated electronic component (12), and the integrated electronic component (12) is installed on the fixed device (13), the fixture (13) is installed on the rim. The thermocouple has a measuring terminal (5) and a pair of electrodes (3), (4). A pair of electrodes (3) and (4) of the thermocouple are twisted and connected to form a measuring end (5), and embedded in the desired measurement point of the tire. ), connected to the integrated electronic assembly (12). The fixing device (13) includes a rim fixing plate (9), a welding post (8), an integrated electronic component fixing plate (7), and the integrated electronic component fixing plate (7) is fixed on the rim fixing plate (9) by the welding post (8). superior. The integrated electronic assembly (12) includes a thermocouple signal receiving circuit, a signal processor, a USB interface, a memory card, a power supply, and a display, and the temperature detection signal lead-out end (6) of the thermocouple is connected to the thermocouple signal receiving circuit, and the integrated electronic assembly (12) Installed on the fixed plate (7) of the integrated electronic assembly.