The invention belongs to the field of construction coating and particularly discloses light-storing long-afterglow light-emitting coating and a preparation method thereof. The light-storing long-afterglow light-emitting coating comprises a light emitting layer and a light reflecting layer. The light emitting layer comprises, by weight, 20-60 parts of organic resin, 15-25 parts of ethanol, 10-50 parts of light-storing powder, 0.3-1.7 parts of octadecanamide, 0.5-5 parts of powdered PED wax, 0.1-1.5 parts of coupling agent, 0.2-1.6 parts of stabilizing agent, 0.1-1.5 parts of antioxidant, 5-25 parts of fluorescent whitening agent, 1-5 parts of fumed silica and 1-5 parts of sodium tripolyphosphate. The light reflecting layer comprises, by weight, 10-30 parts of epoxy resin, 30-60 parts of filler, 0.2-2 parts of film-forming auxiliary, 0.5-4 parts of pigment, 5-20 parts of quartz material, 2-4 parts of organic bentonite and 5-15 parts of dimethyl benzene. The light-storing long-afterglow light-emitting coating is long in light emitting time, fast in drying and high in brightness.