The invention relates to the field of bactericide detection technologies, in particular to a method of detecting bactericide residues in food through a Raman enhanced spectrum. The method comprises the steps that
cut-up samples are taken and placed into an extraction
solvent, an extracting solution is obtained after ultrasonic extraction, and the ratio of the samples to the extraction
solvent is 1g:5ml; after an extracting agent is added into the extracting solution for oscillating extraction, standing is performed for layering,
sodium sulfate is added after extracted supernatant is taken, standing is performed for layering after oscillation, and supernatant is taken and placed in a volatilizing
bottle to volatilize to be dry, wherein the volume ratio of the extracting solution to the extracting agent is 1:1; oscillation is performed after an
organic solvent is added into the volatilizing
bottle, and a sample to-be-detected solution is obtained; and the sample to-be-detected solution,
sol-type nano-gold and an aggregating agent are sequentially added into an SERS tube, and a Raman
spectrograph is used for detection after uniform mixing. Through the method, difenoconazole,
tebuconazole,
pyrimethanil and other bactericides in the food can be identified quickly and sensitively, the pretreatment procedure is simple, and therefore the method has broad application space in the food
rapid detection field.