130results about How to "Guaranteed to be pure" patented technology

The invention discloses a method for producing a live swine fever vaccine by using a cell line. The present invention comprises the following technical steps: (1) selecting a cell line as the cells for making seedlings; (2) subculture and cultivation of cells for making seedlings; (3) breeding of cytotoxic species; (4) breeding of venom for making seedlings; 5) Mixing seedlings, subpackaging and freeze-drying. The invention has the advantages of simple and stable production process, easy operation, high virus content, small difference between batches, easy quality control, and can significantly improve the yield and quality of vaccines. The live swine fever vaccine produced by the invention has good safety and high immune efficacy, and has complete immune protection against the virulent attack of swine fever.

The invention discloses a method for preparing live vaccines of hog cholera and a product thereof. The preparation method comprises the following steps of: (1) culturing porcine passage cell lines; (2) inoculating the porcine passage cell lines with live vaccine production seed viruses of the hog cholera to obtain attenuated vaccine strains of the hog cholera; (3) performing virus multiplication on the attenuated vaccine strains of the hog cholera; (4) measuring the virus titer of multiplication virus suspension by adopting an immunofluorescence method; and (5) adding a freeze-drying protective agent and antibiotics into the virus suspension which is detected to be qualified for vaccine matching and freeze-drying. The preparation method has the advantages of producing the live vaccines of the hog cholera by using the cell lines so as to achieve small quality differences among batches and the characteristics of simple and stable process, easy operation, high yield, low cost, the feasibility and extendibility of industrial production and the like, and measuring the virus titer of the multiplication virus suspension by adopting the immunofluorescence method so as to achieve sensitive, fast, specific and accurate detection, high repeatability and reliable results. The live vaccines of the hog cholera prepared by the method can completely protect pigs from the attacks of violent hog cholera viruses.

The invention discloses an online environment intelligent deployment system and method of a CTF online competition platform. The system includes an online competition platform server terminal, a reverse proxy server terminal, a virtual machine management platform server terminal and several virtual machines created by means of a virtual machine management platform and used for operating a question environment; the online competition platform server terminal is provided with an environment generation module and an environment recycling module, the virtual machines used for operating the question environment are created by means of the virtual machine management platform server terminal according to question configuration information, and port proxy is conducted through the reverse proxy server terminal so that the question environment virtual machines can be accessed through the public network IP and a port. According to the system and method, real-time intelligent deployment of the question environment is achieved through the virtualization technology, the question environment is accessed by deploying a reverse proxy on a limited public network address machine, at the same time, the Internet for the question environment is configured to be independent, it is ensured that the environment can be accessed and isolated, and the safety of other property is ensured.

The invention discloses a cultivation method of Gastrodia elata Bl., and belongs to the technical field of Gastrodia elata Bl. planting. The method comprises the steps of preparation of an armillaria mellea culture medium, preparation of fungus sticks, inoculating, Gastrodia elata Bl. cultivation, management and the like. Due to control of an armillaria mellea culture process, and improvement of a Gastrodia elata Bl. cultivation method, the cultivation method provided by the invention has the advantages that the Gastrodia elata Bl. productivity is improved; Gastrodia elata Bl. cultivated according to the cultivation method is uniform in size, full, and good in appearance.

The invention discloses a production method for vaccines of avian influenza virus and other influenza virus such as swine influenza, dog influenza and equine influenza, which comprises the following steps of: (1) transfer of culture and cultivation of vaccine-made cells; (2) reproduction of vaccine-made virus seeds; (3) microcarrier suspension culture of MDCK cells in a bioreactor; (4) reproduction of vaccine-made virus liquid; and (5) harvest of the virus liquid. The production method has the advantages of reducing the production cost greatly and improving the yield and quality of the vaccines obviously, along with short production period, no restriction to raw material supply, small occupied area, easy and quick expansion of production scale, light environmental pollution, easy processing, high automaticity, low employment and easy realization of balanced and stable quality.

The invention discloses a preparation method of radix tetrastigme powder. The preparation method comprises the following steps of 1, pretreatment: taking a fresh radix tetrastigme medical material, removing impurities, carrying out cleaning by water, and cutting the fresh radix tetrastigme medical material into slices or filing the fresh radix tetrastigme medical material into powder, 2, low-temperature refrigeration, 3, low-temperature crushing, 4, low-temperature vacuum microwave drying, 5, low-temperature air-flow crushing, 6, resonance grinding, and 7, split charging and packaging. The preparation method can realize complete breaking of cell walls of radix tetrastigme, complete release of various ingredients in radix tetrastigme cells, greatly improves penetration, realizes maximum absorption of effective ingredients and is conducive to medicinal action.

The invention discloses an electrospun fiber-reinforced calcium phosphate bone cement composite material and application thereof. The electrospun fiber-reinforced calcium phosphate bone cement composite material is formed by mixing electrospun fiber, a chitosan solution and a solid phosphate mixture, and is prepared by the following ways: directly spinning the electrospun fiber in the chitosan solution containing chitosan, citric acid and glucose; then, mixing with the solid phosphate mixture to obtain the electrospun fiber-reinforced calcium phosphate bone cement composite material. The electrospun fiber-reinforced calcium phosphate bone cement composite material disclosed by the invention, on the basis of reserving advantages of the calcium phosphate bone cement, is relatively high in compressive strength in an initial stage, and a porous scaffold material is formed with gradual degradation of the electrospun fiber in a later stage, which is favorable for growth of a new bone tissue.

The invention discloses a method for producing a curative canine parvovirus virus monoclonal antibody by using a bioreactor.

The invention discloses a refractory castable material special for tertiary air duct gates, comprising the following components according to the weight percentage: 70-75% of mullite M70, 5-10% of aluminum oxynitride (AlON), 6-7% of pure calcium aluminate cement, 3-4% of a-Al2O3, 3-5% of silica fume and 5-7% of water. During preparation, dodecacalcium heptaluminate (C12A7) with mass being 2% of the total mass of the mixed six components is additionally added. The refractory castable material special for tertiary air duct gates of the invention has extremely high wear-resisting property and thermal shock resistance. By adding AlON nano fine powder as an antioxidant, high thermal resistance and high temperature resistance to chemical erosion of products are improved; by increasing low temperature oxide of sodium oxide and reducing the sintering temperature of the castable material, the strength and wear-resisting property of products are improved; by adding C12A7 to adjust stripping time of the castable material, different requirements on the construction time and customers are met.

The invention belongs to the field of agricultural technology, and particularly relates to an organic Italian lettuce cultivating method. By the organic Italian lettuce cultivating method, organic Italian lettuce is cultivated from a non-replicable seed variety in Italian through seed treatment, nutritional soil preparation, seedbed preparation, sprouting and seedling cultivation, land preparation and furrowing, field planting and cultivation, field management, and harvest in due time. The seeds are good in characteristics, so that purity and mouth feel of a product can be ensured; in the growth process, the field management adopts relatively strict operating rules; fertilizer applying and watering periods are strictly controlled in accordance with the growth process of the lettuce, thus meeting the needs on water and fertilizer in the growth process of the lettuce; in the growth process of the lettuce, various trace elements are sprayed so as to ensure the content of trace elements in the lettuce, thus improving the nutritional value of the product; during the production process, biological and physical control technologies and agricultural control technology are fully used, and during the whole production process, fertilizer is forbidden and biologic fertilizer and organic manure are promoted overall; by the organic Italian lettuce cultivating method, the lettuce quality is good, the germination rate is high, the product is good in mouth feel and the nutritional value is high.

The present invention discloses a donkey-hide gelatin cake. The donkey-hide gelatin cake consists of the following raw materials: 450-650 parts of donkey-hide gelatin, 450-650 parts of yellow rice wine, 350-450 parts of walnuts, 450-550 parts of black sesame seeds, 130-200 parts of crystal sugar, 350-450 parts of red jujubes, 110-190 parts of longan meat and 100-150 parts of Chinese wolfberry fruits. By rationally combining each of the raw materials, the effectiveness is combined. The donkey-hide gelatin cake has efficacies of supplementing qi and tonifying blood, beautifying features and nourishing skins, nourishing yin and supplementing kidneys, moistening intestines and relaxing bowels, consolidating basis, regulating qi and relieving depressions, improving sleep, regulating menstruation and relieving pain, and preventing miscarriage, can be used to treat the symptoms of qi and blood deficiency, dizziness, palpitations and insomnia, yellow face and white lips, energy fatigue, appetite loss, lung dryness and cough, female menstruation pain, frozen hands and feet, abnormal vaginal discharge, etc., and can prevent osteoporosis, prevent senile diseases, prevent climacteric syndrome, delay aging, enable hair to be black and bright, and improve immunity in a long-term use.

The invention provides an electronic cigarette capable of automatically detecting E-liquid supply and a work method thereof. The electronic cigarette comprises an atomizer, a control pump, a circuit board and a battery. The atomizer comprises a base, an outer shell, an inner shell and an atomizing core, a receding groove is formed between the inner shell and the outer shell, an E-liquid detectingpart is arranged in the receding groove, an E-liquid passing hole is formed in the inner shell, and the atomizing core is arranged in the inner shell. An E-liquid storage bin is located below the atomizer. The control pump is provided with an E-liquid inlet and an E-liquid outlet, the E-liquid inlet is connected with the E-liquid storage bin through a first pipeline, and the E-liquid outlet is connected with the receding groove through a second pipeline. The circuit board is connected with the control pump, and controls work of the control pump. The battery is connected with the control pump and the circuit board. Precise control over the amount of E-liquid is achieved through cooperation of the control pump, the circuit board and the E-liquid detecting part, the E-liquid is supplied instantly when needed, the E-liquid is not supplied when not needed, dry burning and E-liquid exploding can be effectively avoided, and it is guaranteed that the taste of the atomized E-liquid is pure.

The invention discloses a method for producing a vaccine for avian influenza viruses and other influenza viruses such as a swine influenza virus, a canine influenza virus and an equine influenza virus. The method comprises the following steps of: subculturing cells for preparing the vaccine; (2) reproducing cytotoxic varieties; (3) performing microcarrier suspension culture on darby canine kidney (MDCK) cells in a bioreactor; (4) reproducing venom for preparing the vaccine; and (5) harvesting virus liquid. The method has the advantages of great reduction in production cost, short production period, no restriction to raw material supply, small occupied area, easy and quick expansion in production scale, low and readily treated environmental pollution, high degree of automation, few labors, easy equilibrium and stabilization in quality and obvious improvement on the yield and the quality of the vaccines.

A reactive disperse dye one-bath polyester cotton blended yarn dyeing technology belongs to the field of polyester cotton dyeing technology. The technology is characterized by comprising the following steps: pretreatment, one-bath dyeing and aftertreatment. The one-bath dyeing comprises the following steps: adjusting temperature to 38-45 DEG C after polyester cotton blended yarn enters dye liquor, adding sodium sulfate, and running for 9-11 min; adding reactive dye and disperse dyes and running for 9-11 min, adding residual auxiliary agent and running for 9-11 min, heating at the heating rate of 0.95 DEG C / min-1.1 DEG C / min to the temperature of 63-67 DEG C, running for 27-33 min, heating at the heating rate of 0.95 DEG C / min-1.1 DEG C / min to the temperature of 128-133 DEG C, and running for 37-43 min so as to finish the dyeing. By the one-bath, dyeing of two fibers is realized. By the technology, production efficiency is greatly enhanced, energy consumption is greatly reduced, and one-bath colour shade difference is eliminated.

The longan-chrysanthemum tea beverage with radiation resisting effect consists of main material and supplementary material. The main material includes green tea with tea polyphenol and tea polysaccharide to reduce or eliminate radiation harm; four kinds of medicinal food material including longan, chrysanthemum, Coix seed and licorice with health function and no toxic side effect; and deoxygenated deionized water to ensure the pure color and taste of tea beverage. The supplementary material includes sugar to correct taste, vitamin C as antioxidant to ensure the pure color, smell and taste of tea beverage, and sodium bicarbonate to regulate pH value of tea beverage and protect catechin component against destruction.

The invention provides a nanometer taxus chinensis product and a preparation method thereof. In the method, the taxus chinensis is taken as a raw material, the latest nano process technology and pure physical method are utilized and the taxus chinensis is crushed into powder through mechanical forces, wherein the average grain diameter of the powder particle is 1-1000 nanometer, thus the cellwall of the taxus chinensis is completely broken, the various components in the taxus chinensis are completely released, the permeability is improved greatly, and the effect and the curative effect are both improved substantially. The invention makes full use of the root, stem, leaves, trunk and bark of the taxus chinensis, is close to zero wastage of raw materials during process, and can greatly save the taxus chinensis resources.

The present invention discloses an old ginger paste. The old ginger paste comprises the following raw materials in parts by weight: 200-350 parts of old gingers, 100-220 parts of red jujubes, 200-350 parts of walnuts and 450-650 parts of brown sugar. The old ginger paste reasonably combines each of the raw materials, so that the effectiveness of the raw materials supplement each other and the old ginger paste has efficacies of dispelling wind and inducing perspiration, stimulating spleen and stomach, supplementing blood and tonifying qi, removing cold and dampness, raising blood qi, etc., and can be used in treating exogenous wind-cold, headache, phlegm and retained fluid, cough, stomach cold vomiting, menstrual pain, etc. In addition, the sweetness of the brown sugar, red jujubes and other raw materials in the old ginger paste neutralize the spiciness of the old gingers, so that the old ginger paste is very good in tastes, can be consumed directly, and is suitable for people at all age levels to eat. In addition, the present invention also discloses a manufacturing method of the old ginger paste.

The invention discloses a square filtering tank for a beer saccharification filtering system, and relates to a filtering system special for beer saccharification. The square filtering tank comprises a filtering plate, an exhaust barrel, a vinasse discharging lifting device, a vinasse raking reciprocating device, a tank washing water inlet, a washing water inlet, supporting legs, a mash inlet, a backwashing water inlet and a wheat juice outlet, wherein the exhaust barrel is fixed at the top of a tank body; the vinasse discharging lifting device and the vinasse raking reciprocating device are arranged on the tank body in a sliding manner and are both connected with a large vinasse raking arm in the tank body; the tank washing water inlet is also formed in the tank body; the washing water inlet is formed in the side surface of the tank body; the mash inlet is formed below the washing water inlet; the filtering plate is arranged in the tank body; a vinasse discharging door is arranged on the filtering plate; the backwashing water inlet and the wheat juice outlet are formed in the lower end of the tank body. According to the square filtering tank for the beer saccharification filtering system, the linear speeds of raking cutters in the tank during vinasse raking are consistent, so that the original concentration of the collected wheat juice at different positions after mash is filtered is the same, and the pure flavor of wheat juice is guaranteed.

A hemp milk and a preparation method for the same are disposed, wherein the prescription of the hemp milk comprises the following raw materials in percentage by weight: 5-60% of hempseed raw pulp, 6-20% of cane sugar, 0-20% of ingredient juice, 0.2-0.6% of compound emulsification stabilizer and the balance of purified water; the hempseed raw pulp is the pulp obtained by mixing and pulping hempseeds and purified water in a weight ratio of 1: 1-10 and then filtering; and the compound emulsification stabilizer is a mixture containing one or more materials selected from monostearin, sucrose fatty acid ester, water-soluble monostearin, sodium alginate and carboxymethyl cellulose sodium. The preparation method comprises the following steps of: adding water to dissolve compound emulsification stabilizer dry powder while stirring; adding cane sugar and previously-prepared hempseed raw pulp; adding ingredient juice as needed; and uniformly mixing to obtain a hemp milk. The invention provides a hemp milk drink prominent in healthcare function and good in taste, filling in the gap of market, and capable of satisfying the increasing living requirements of people.

The invention belongs to the field of food manufacture, and provides a method for producing green plum impregnated wine. The method comprises the following steps: (1) green plum treatment; (2) green plum impregnation; (3) green plum wine aging; (4) green plum wine subpackage. A large quantity of tests show that if green plums are stored for 20 to 24 hours at 20 to 22 DEG before impregnation, the acidity of the green plums can be lowered, so as to taste better. When the aging step is conducted on green plum wine virgin pulp after filtering, impurities of the green plum wine virgin pulp can be reduced, the transparency of the green plum wine can be improved, and the green plum wine can be more stable. When the aging step is conducted on green plum wine virgin pulp after high-temperature instant thermal treatment, and the sealing step is carried out after ultrasonication and thermal treatment, the stability of the green plum wine also can be improved; moreover, high-temperature instant thermal treatment and ultrasonication also enable the taste of the green plum wine better and the flavor of the green plum wine to be more typical. Overall, through the large quantity of tests, the green plum impregnated wine which is unique in flavor, excellent in quality, and stable in property can be obtained. The method provides a reference for manufacturers to produce green plum impregnated wine.

The invention relates to the technical field of EVA composite materials. The natural aromatic environment-friendly EVA composite foam material is prepared from the following components in parts by weight: 80-100 parts of a rubber and plastic base material, 4-8 parts of a foaming agent, 0.5-1 part of a cross-linking agent, 0.5-0.8 part of mineral oil, 0.5-1 part of stearic acid and 20-40 parts of modified lavender particles, and the rubber and plastic base material contains 40-100% of EVA. According to the invention, the modified lavender particles are adopted, volatilization of lavender aromacomponents can be effectively controlled, meanwhile, the antibacterial performance and deodorization of the foaming material and the product can be enhanced, the advantages of deodorizing, ventilating, refreshing, incensing, expelling parasites and the like are achieved, the utilization rate of lavender can be increased, waste and environmental pollution are reduced, and the lavender perfume is environmentally friendly and suitable for living goods and sports equipment such as soles, insoles, yoga mats and crawling mats.

The invention provides a method for producing a therapeutic monoclonal antibody of a canine distemper virus, a product thereof and a hybridoma cell. The method provided by the invention comprises the following steps: (1) carrying out amplification culture on the hybridoma cell which secretes and produces the therapeutic monoclonal antibody of the canine distemper virus in a cell culture solution; (2) then, inoculating the hybridoma cell into a cell culture solution in a bioreactor and culturing; (3) harvesting the therapeutic monoclonal antibody, produced by the hybridoma cell, of the canine distemper virus. The method provided by the invention has the advantages that the produced therapeutic monoclonal antibody of the canine distemper virus is high in purity, little in batch difference and easy in quality control, and the quality of the therapeutic monoclonal antibody can be improved remarkably.

The invention discloses a method of cultivating Gastrodia elata and belongs to the technical field of planting of Gastrodia elata. The method comprises the steps of preparing Armillaria mellea, preparing mushroom sticks, inoculating, cultivating Gastrodia elata, and managing. By controlling the cultivation process of Armillaria mellea and modifying the method of cultivating Gastrodia elata, the yield of Gastrodia elata is increased; tubers of Gastrodia elata cultivated through the method are uniform in size, full and good in condition.

The invention discloses a preparation method and product of a swine fever live vaccine. The preparation method comprises the following steps: (1) culturing a swine-derived continuous cell line; (2) inoculating the swine-derived continuous cell line into a seed virus for producing the swine fever live vaccine, thereby obtaining a swine fever weak-virus vaccine strain; (3) carrying out virus multiplication on the swine fever weak-virus vaccine strain; (4) determining the virus value of the multiplied virus suspension by an immunofluorescence method; and (5) adding a freeze-drying protective agent and antibiotics into the qualified virus suspension to carry out vaccine preparation and freeze-drying. Since the swine fever live vaccine is prepared from the cell line, the difference of quality among different batches is small, and the invention has the characteristics of simple and stable technique, high yield, low cost and the like, is easy to operate, and has feasibility and amplification of industrialized mass production. In addition, the immunofluorescence method, which has the advantages of high detection sensitivity, high speed, high specificity, high accuracy, high repetitiveness and reliable result, is used for determining the virus value of the multiplied virus suspension. The swine fever live vaccine disclosed by the invention can 100% protect the attack of swine fever strong virus.

A production method of Newcastle disease (ND) vaccine includes (1) adding virus growth solution to chick-embryo-cultured inoculation subculture cell line of attenuated ND virus, and culturing to obtain cell-adapted vaccine seed virus; adding virus culture maintenance medium to inoculation subculture cell line of cell-adapted vaccine seed virus, and culturing to obtain proliferated virus suspension; (3) determining titer of the proliferated virus suspension, preparing vaccine from qualified suspension, sub-packaging, and lyophilizing. The production method has advantages of simple and stable production process, easy operation, high virus content, small batch difference, easily controlled quality, rapid and accurate titer determination, improved vaccine yield and quality, high vaccine safety, high immune efficacy, and complete immuno-protection effect against ND virus.

The invention relates to a welding method of a C-SiC sputtering target material. The welding method comprises the steps of (1) carrying out shielding treatment on the C-SiC target material and a backplate, then placing solder on welding surfaces of the C-SiC target material and back plate which are subjected to the shielding treatment, carrying out heating, and carrying out soaking treatment on the welding surfaces of the C-SiC target material and the back plate after the heating is completed to obtain the treated C-SiC target material and back plate; (2) arranging a molten pool on the treated back plate obtained in the step (1), and simultaneously placing solder and arranging copper wires at equal intervals in the molten pool to obtain the treated back plate; and (3) carrying out buckling assembly and welding on the treated C-SiC target material obtained in the step (1) and the treated back plate obtained in the step (2). According to the welding method of the C-SiC sputtering targetmaterial, indium slag which has great influence on the welding result on the welding surfaces is removed, and it is ensured that the solder between the target material and the back plate is pure andpollution-free; and through the treatment mode, it is guaranteed that the average one-time welding bonding rate of the C-Scan ultrasonic detection of the C-SiC target material can reach 98% or above,the product processing time is greatly saved, and a large amount of cost is saved.

The invention relates to a method for establishing an exopalaemon carinicauda family by applying in-vitro egg culture. The method comprises the steps that parent exopalaemon carinicauda are selected and disinfected, and the parent exopalaemon carinicauda and eggs are separated; shaking culture parameters of the eggs are measured; microscopic observation is performed in the culture process; the progressive decreasing control of salinity is performed in the culture process; young exopalaemon carinicauda after incubation are cultured. According to the method, the probability of incubation failure caused by influence of different factors in the egg laying process of the parent exopalaemon carinicauda can be effectively lowered, the incubation rate of the eggs and the survival rate of the young exopalaemon carinicauda are increased, and therefore the method has good application prospects in the application of establishing the exopalaemon carinicauda family.

The invention discloses a production method and a production device for bayberry juice; the production method comprises the following steps: cooling the selected fresh bayberry to 8-10 DEG C, and in the inert gas protection atmosphere; firstly, adopting the vacuum negative pressure juicing method for squeezing juice for the fresh bayberry to obtain the bayberry juice; filtering the bayberry juice and cooling the bayberry juice to 5-8 DEG C; and sealing and storing the cooled bayberry juice at 1-5 DEG C. The production device comprises a peristaltic pump, an air bag juice extractor and a storing tank; the outlet of the peristaltic pump is connected with the feeding port of the air bag juice extractor through a food grade soft pipe in a sealing mode, the outlet of the air bag juice extractor is connected with the inlet of the storing tank through a sealing transmission passage, the sealing transmission passage is provided with a filtering device and a cooling device along the transmission direction, and the sealing transmission passage is further provided with the first sanitary pump and the second sanitary pump. The complete nutritional ingredient and color of the bayberry can be preserved for great degree by the production method and the production device, the juice yield is high and the quality of the bayberry juice is good.

The invention relates to an extraction process for a heatable but non-combustible cigarette plant perfume material; the extraction process comprises the following steps of (1) crushing a plant raw material, wherein the mesh number is 40-80 meshes, adding 0.5-1 time water to plant powder and uniformly mixing; (2) placing a mixture in a retorting device, and carrying out heating cracking at different temperature gradients; and (3) collecting the product of gas and liquid through a condensation collection device at an outlet end of the retorting device, thereby obtaining the perfume material suitable for the heated but non-combustible cigarette. According to the extraction process disclosed by the invention, extraction is carried out according to a cracking principle of heating but non-combustion, and fragrance substances are reduced to a heated but non-combustible cigarette smoke formation section, so that the fragrance of the plant raw material is ensured to be pure, and no chemical atmosphere is contained.

The invention provides a method of utilizing a stirred bioreactor to produce an infectious Bursal disease virus. Bioreactor microcarrier cell culture technology is used to replace conventional roller bottle culture, so that the problems of low production efficiency, unstable product quality and low virus titer can be solved. On this basis, biological characteristics of the infectious Bursal disease virus and DF1 cells are combined, and proper conditions are matched from the perspectives of microcarrier adding amount, cell inoculation density, virus inoculation amount, cell density during virus inoculation, virus collection time and reactor operation parameters, so that virus culture efficiency is improved remarkably, and unit culture titer is improved by 10-100 times. In addition, compared with roller bottle culture, the method utilizing the bioreactor has the advantages that culture scale is large, and parameter control is comprehensive, so that systematic risk of being polluted is lowered, quality stability is improved, and the method has a wide application prospect.