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Method for producing curative canine parvovirus virus monoclonal antibody by using bioreactor

A monoclonal antibody, canine parvovirus technology, applied in the fields of biochemical equipment and methods, microorganisms, anti-animal/human immunoglobulin, etc., can solve the problems of long production cycle, large differences between batches, incompatibility, etc.

Active Publication Date: 2011-07-13
北京世纪元亨动物防疫技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, this method has low yield, long production cycle, and large batch-to-batch variation, which is not suitable for mass production of therapeutic monoclonal antibodies

Method used

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  • Method for producing curative canine parvovirus virus monoclonal antibody by using bioreactor
  • Method for producing curative canine parvovirus virus monoclonal antibody by using bioreactor

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Embodiment Construction

[0008] In one embodiment, the present invention provides a method for producing a therapeutic canine parvovirus monoclonal antibody, comprising the steps of:

[0009] (1) Inoculate the hybridoma cells secreting anti-canine parvovirus monoclonal antibody into the culture medium in the bioreactor;

[0010] (2) cultivating hybridoma cells in a bioreactor;

[0011] (3) Harvest the anti-canine parvovirus monoclonal antibody produced by the hybridoma cells.

[0012] In the present invention, "bioreactor" refers to a bioreaction device that provides a good closed environment for culturing growing and non-immobilized cells, and is usually also called a cell fermenter. A variety of bioreactors may be suitable for use in the present invention, including, for example, stirred bioreactors, airlift bioreactors, hollow fiber bioreactors, and fluidized bed bioreactors, among others.

[0013] There are three main types of bioreactor cell culture: batch, fed-batch and perfusion. In simple b...

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Abstract

The invention discloses a method for producing a curative canine parvovirus virus monoclonal antibody by using a bioreactor.

Description

technical field [0001] The invention relates to the technical field of monoclonal antibody production, in particular to a method for producing therapeutic canine parvovirus monoclonal antibody with a bioreactor. Background technique [0002] In 1975, Koehler and Milstein (Nature Vol.256, pp 495-497) used hybridoma technology to prepare the world's first mouse monoclonal antibody, which opened up a new field for biotechnology. With the development of biotechnology, monoclonal antibody technology has also made great progress. Today, monoclonal antibodies have been widely used in various fields of biomedicine (including human and veterinary medicine), especially in the field of basic research and clinical application of the prevention and treatment of viral infections, and are increasingly playing an extremely important role. [0003] Canine parvovirus (Canine Parvovirus, CPV) infection is an acute infectious disease in dogs. In 1978, Kelly in Australia and Thomson in Canada ...

Claims

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Application Information

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IPC IPC(8): C07K16/18C12N5/18
Inventor 孙明陈西钊田克恭曹振张丽
Owner 北京世纪元亨动物防疫技术有限公司
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