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Newcastle disease (ND) vaccine, and its production method

A technology of Newcastle disease and production method, which is applied in the field of live Newcastle disease vaccine production, can solve the problems of vaccine yield and efficacy improvement, the inaccurate quantification of virus detection, and the influence of culture conditions, etc., and achieve good economic benefits and application prospects. Accurate determination Vaccine titer, quality control effect

Inactive Publication Date: 2012-07-25
SINOVET BEIJING BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using SPF chicken embryos to produce live Newcastle disease vaccine is costly, low labor utilization rate, easy to cause exogenous virus contamination, and large batch-to-batch differences, which brings difficulties to the improvement of vaccine production and efficacy
[0003] In addition, the height of the vaccine titer is a key indicator of whether the vaccine is qualified or not. In the prior art, the SPF chicken embryo method (EID 50 ), the potency measurement method has the defects that the toxicity cannot be accurately quantified, the detection result is easily affected by individual differences in chicken embryos and culture conditions, the test cycle is long, time-consuming and labor-intensive and other defects, and urgently needs to be improved

Method used

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  • Newcastle disease (ND) vaccine, and its production method
  • Newcastle disease (ND) vaccine, and its production method
  • Newcastle disease (ND) vaccine, and its production method

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Example 1 MDCK cell line is used as a vaccine production cell to produce Newcastle disease attenuated live vaccine

[0032] (1) Select the MDCK cell line (purchased from ATCC, USA) as the cells for making seedlings;

[0033] (2) Passage and culture of cells for seedling production: the above-mentioned MDCK cell line was digested and passaged with EDTA-trypsin cell dispersion solution, and continued to be cultured with cell growth medium at 37°C. When a good monolayer was formed, it was used for continued passage or virus inoculation ;

[0034] (3) Propagation of cytotoxic species: the well-grown MDCK cell line monolayer was washed twice with cell maintenance solution or PBS, and the cytotoxic species (Newcastle disease attenuated La sota strain; purchased from China Veterinary Drug Control Institute) was inoculated with the above-mentioned Cell line monolayer, 37 ℃ for 1 hour; add virus culture and maintenance solution at 37 ℃ to continue culture, 32-40 hours when 80% ...

Embodiment 2

[0043] Embodiment 2vero cell line is used as the cell production Newcastle disease attenuated live vaccine for making vaccine

[0044] (1) Select the vero cell line (purchased from ATCC, USA) as the cells for making seedlings;

[0045] (2) Passage and culture of cells for seedling production: the above-mentioned vero cell line was digested and passaged with EDTA-trypsin cell dispersion solution, and continued to culture with cell growth medium at 37°C. When a good monolayer was formed, it was used for continued passage or virus inoculation ;

[0046] (3) Propagation of cytotoxic species: the well-grown vero cell line monolayer was washed twice with cell maintenance solution or PBS, and the inoculated cytotoxic species (ZM10 strain; purchased from China Veterinary Drug Administration) was inoculated with the above cell line monolayer. layer, 37°C for 1 hour; add virus culture maintenance solution at 37°C to continue culturing, 30-40 hours when 80% of the cells fall off, collec...

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Abstract

A production method of Newcastle disease (ND) vaccine includes (1) adding virus growth solution to chick-embryo-cultured inoculation subculture cell line of attenuated ND virus, and culturing to obtain cell-adapted vaccine seed virus; adding virus culture maintenance medium to inoculation subculture cell line of cell-adapted vaccine seed virus, and culturing to obtain proliferated virus suspension; (3) determining titer of the proliferated virus suspension, preparing vaccine from qualified suspension, sub-packaging, and lyophilizing. The production method has advantages of simple and stable production process, easy operation, high virus content, small batch difference, easily controlled quality, rapid and accurate titer determination, improved vaccine yield and quality, high vaccine safety, high immune efficacy, and complete immuno-protection effect against ND virus.

Description

technical field [0001] The invention relates to a production method of an animal vaccine, in particular to a production method of a chicken Newcastle disease live vaccine and a chicken Newcastle disease live vaccine obtained by the production method, and belongs to the field of production of chicken Newcastle disease live vaccines. Background technique [0002] China currently produces low-virulence Newcastle disease live vaccines that use SPF chicken embryos. At present, the production capacity and quality of domestic SPF chicken embryos restrict the production of livestock and poultry biological products. The cost of producing Newcastle disease live vaccine with SPF chicken embryos is high, the labor utilization rate is low, and it is easy to cause foreign virus contamination, and the difference between batches is large, which brings difficulties to the improvement of vaccine production and efficacy. [0003] In addition, the height of the vaccine titer is a key indicator...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/17A61P31/14
Inventor 武华王洪林
Owner SINOVET BEIJING BIOTECH
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