The invention belongs to the field of
organic synthesis, and
analytical chemistry and particularly relates to a preparation method and an application of a bitertanol based molecularly-imprinted
solid-phase extraction column. The traditional
solid-phase extraction column has the disadvantages that the actual
recovery rate of
analyte is low, the interaction between the
solid-phase extract and the adsorbent is nonspecific, and the extraction and
elution conditions are severe. In addition, the traditional solid-phase extraction column has poor selectivity, can lead to the incomplete treatment of impurities and the
pollution to the
chromatographic column easily and can not be used repeatedly. The bitertanol based molecularly-imprinted
polymer is prepared by adopting a
mass polymerization method, after the prepared molecularly-imprinted
polymer is subjected to a series of treatments, such as
grinding, rinsing, sieving, eluting and
drying, the molecularly-imprinted
polymer is evaluated and analyzed by adopting the
ultraviolet spectrophotometry and the high-performance liquid
chromatography to so as to have a
specific adsorption effect, and the molecularly-imprinted solid-phase extraction column is prepared. The molecularly-imprinted solid-phase extraction column is used for purifying a sample, and separating and enriching the bitertanol and structural analogues thereof in food and feed.