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123 results about "Uv spectrophotometry" patented technology

Method for culture of alpinia galanga and its quality-control

A method for culturing the rhizome of alpinia galanga includes such steps as tissue culture, transplanting, field management, applying fertilizer, controlling diseases and pests and harvesting. Its processing, transportation and storage are also disclosed. Its quality control method includes UV spectrophotometry, thin-layer chromatography, gas-phase chromatographic fingerprint, and efficient liquid-phase chromatography for measuring the content of 1'-acetoxychavicol acetate.
Owner:HONGYI SCI & TECH CO LTD NANCHANG

Method for rapidly and quantitatively determining the triterpenoid content in ganoderma lucidum

The invention is a method for measuring the content of triterpenes compound in Ganoderma Lucidum, especially a method for using ultraviolet splitting luminosity to measure, the invention refers to biology technology field. The technology project is: the Ganodeerma Lucidum is put into a cleaned tube, adds chloroform to immerse for 0.5-1 hours, and acquires the supernate after being centrifugated, uses the saturate NaHCO3 solvent to extract for 2-3 times, collects the NaHCO3 layer, acquires the liquid for test, measures the volume accurately, uses ultraviolet splitting luminosity method to measure the light absorbing valve under 250-260nm wavelength, works out the content of triterpenes in the sample according to the standard curve. The method saves the acidification, chloroform extraction, reduced pressure distillation and drying steps, the period of measurement is shortened greatly.
Owner:无锡市三联高科技开发有限公司

Preparation method of antioxidant in blueberry leaves

ActiveCN102178325AStrong total antioxidant capacityStrong purityFood preservationWater bathsAlcohol
The invention belongs to a preparation technology of natural antioxidant, particularly relates to a preparation method of antioxidant in blueberry leaves. The preparation method of the antioxidant in the blueberry leaves includes the steps as follows: (1) collecting fresh blueberry leaves, washing the fresh blueberry leaves with water, draining away water, shearing the leaves into segments with different length, adding 8-14 times water, adjusting pH to 3-8, conducting extraction in hot-water bath at the temperature of 90-100 DEG C, filtering the extract, and decompressing and concentrating the extract to 15-25 times of original concentration at the temperature of 50-60 DEG C; and (2) conducting adsorption with macroporous resin, eluting with water and 10%-30% alcohol, detecting by ultraviolet spectrophotometry, collecting adsorbed eluent with strong antioxidant activity at 280nm part, decompressing and concentrating the collected eluent to paste, and conducting vacuum-drying at the temperature 50-60 DEG C, thus obtaining light brown powder. The preparation method is simple in technology, friendly to environment and good in purity; and the product is safe and has strong antioxidantactivity.
Owner:海宁市袁花镇工业投资有限公司

Method for detecting fluorescent brightener in food and paper packaging thereof

The invention discloses a method for detecting a fluorescent brightener in food and paper packaging thereof. The method comprises the following steps: (1) preparing fluorescent brightener standard solutions with different concentrations; (2) preparing a standard curve between the fluorescent brightener concentration and absorbancy; (3) extracting a fluorescent brightener; and (4) arranging the filtrate in the step (3) in a quartz cuvette being 1cm, measuring the absorbancy at 350nm by adopting an ultraviolet spectrophotometer, performing a blank experiment, and calculating to obtain the content of the fluorescent brightener in the food and paper packaging thereof according to the standard curve obtained in the step (2). The fluorescent brightener detection in the food and the paper packaging thereof is realized by adopting ultraviolet spectrophotometry, the experimental apparatus is simple, the operation is convenient, and the method has the advantages of quantitative analysis, low detection limit, high repeatability and the like and is suitable for measurement of micro and trace components.
Owner:邢台市产品质量监督检验所

Preparation method and application of bitertanol based molecularly-imprinted solid-phase extraction column

The invention belongs to the field of organic synthesis, and analytical chemistry and particularly relates to a preparation method and an application of a bitertanol based molecularly-imprinted solid-phase extraction column. The traditional solid-phase extraction column has the disadvantages that the actual recovery rate of analyte is low, the interaction between the solid-phase extract and the adsorbent is nonspecific, and the extraction and elution conditions are severe. In addition, the traditional solid-phase extraction column has poor selectivity, can lead to the incomplete treatment of impurities and the pollution to the chromatographic column easily and can not be used repeatedly. The bitertanol based molecularly-imprinted polymer is prepared by adopting a mass polymerization method, after the prepared molecularly-imprinted polymer is subjected to a series of treatments, such as grinding, rinsing, sieving, eluting and drying, the molecularly-imprinted polymer is evaluated and analyzed by adopting the ultraviolet spectrophotometry and the high-performance liquid chromatography to so as to have a specific adsorption effect, and the molecularly-imprinted solid-phase extraction column is prepared. The molecularly-imprinted solid-phase extraction column is used for purifying a sample, and separating and enriching the bitertanol and structural analogues thereof in food and feed.
Owner:HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY

Quality controlling method of ganoderma aqueous extract

The invention relates to a quality analysis analysis method of medicines or health care products, and specifically relates to a quality controlling method of a ganoderma aqueous extract. For controlling the product quality with high specificity, according to the invention, crude polysaccharide contents and a sum of ganoderma acid A, B, and C2 contents are adopted as quality controlling indexes of the ganoderma aqueous extract. According to the invention, ganoderma crude polysaccharide is extracted with a water extraction and alcohol precipitation method; polysaccharide with a glucan structure is precipitated by using a copper reagent; and the content of the polysaccharide is determined by using an ultraviolet spectrophotometry method. Therefore, the influences of auxiliary materials on determination are eliminated, and a basis is provided for quality controlling of the ganoderma aqueous extract. The contents of the characteristic components ganoderma acid A, B, and C2 in ganoderma lucidum, ganoderma sinnese, and ganoderma tsugae are relatively high. Compared to a currently commonly-used colorimetric method for determining total triterpenoid contents, the specificity of the method provided by the invention is high. With the method, purposes of authentication and quality control can be achieved.
Owner:福建省食品药品质量检验研究院 +1

Preparation for treating hysteritis and its quality control method

A Chinese medicine Gongyanping for treating gynecological inflammations is prepared from 5 Chinese-medicinal materials including shing bugleweed herb, zanthoxylum nitidum, Chinese angelica root, hispid fig root, etc. Its quality control method features that the efficient liquid-phase chromatographic analysis method or UV spectrophotometric method is used for measuring the content of tannin or gallic acid, and the thin-layer method is used to measure the contents of the one or more active components contained in its raw materials.
Owner:GUANGDONG LUOFUSHAN SINOPHARM

Quality control method of medicinal preparation for treating gynecological inflammation and hysteromyoma

The invention discloses a quality control method of a medicinal preparation for treating gynecological inflammation and hysteromyoma. The quality control method of the capsules comprises character, identification, examination and content detection, wherein the identification is to identify bittersweet herb, spreading hedyotis herb, sowthistle tasselflower herb, asiatic pennywort herb and herb of common goldernrod; the content measurement is to measure the chlorogenic acid content and total flavones content of the preparation by using high performance liquid chromatography and UV spectrophotometry respectively. The quality control method of the invention is scientific, reasonable, high in accuracy and high in repeatability, can completely and effectively control the quality of the capsules for treating gynecological inflammation and hysteromyoma to assure the clinic effectiveness of the preparation.
Owner:贵阳春科药业技术研发有限公司

Method of detecting contents of cellulose, hemicellulose and lignin of corn stovers

The invention relates to a method of detecting contents of cellulose, hemicellulose and lignin of corn stovers. The method comprises: 1, taking treated corn stovers, crushing, and drying to obtain the corn stover powder; 2, detecting the content of the lignin of the corn stover powder; and 3, detecting the contents of the hemicellulose and the lignin of the corn stover powder. The method combines a UV spectrophotometry method and an HPLC method to detect the contents of the cellulose, the hemicellulose and the lignin of the corn stovers, is simple, easy to operate and high in accuracy, and has a positive significance for quantification of biomass energy use and development of related equipment.
Owner:SHANGHAI JIAO TONG UNIV

Gynostemma total saponin and preparation method thereof

The invention relates to gynostemma total saponin and a preparation method thereof, belongs to the field of traditional Chinese medicine and relates to a Chinese medicine active part and a extracting, purifying and refining method thereof. Gynostemma herbs are placed in a reflux extractor to be subjected to reflux extraction with high-concentration ethanol prior to filtration and alcohol removal to obtain a non-alcohol filtrate, the filtrate is absorbed and eluted with macroporous resin, eluants refer to water, low-concentration alkali, low-concentration alcohol and high-concentration alcohol, the eluants are obtained, and the high-concentration alcohol eluant is retained, concentrated and evaporated to dryness to obtain the gynostemma total saponin. For the prepared gynostemma total saponin, total saponin content measured by gypenosides A with the ultraviolet spectroscopy is 92-94%. The method is high in efficiency, simplified in steps, short in cycle, low in cost and suitable for industrial production.
Owner:SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M

Preparation of beta-cyclodextrin sustained-release microcapsule of forestry insect repellant (E)-2-hexenal and determination method

A preparation of beta-cyclodextrin sustained-release microcapsule of a forestry insect repellant (E)-2-hexenal and a determination method relate to the forestry insect disease control and medicament sustained-release field. According to the preparation method provided by the invention, beta-cyclodextrin is used as a wall material for clathration of the insect repellant (E)-2-hexenal so as to obtain the beta-cyclodextrin microcapsule with a sustained-release effect. Air at a constant rate is continuously blown in for 30 days, and the release amount of (E)-2-hexenal during 24 hours is quantificationally measured by UV spectrophotometry. Results show that the beta-cyclodextrin microcapsule prepared by the method has a good release effect and can be used to control insects in the forestry field.
Owner:SOUTHWEST FORESTRY UNIVERSITY

Method for determining index composition content of Qingkailing injection intermediate body and finished product

The invention relates to a method for measuring index component content in a Qingkailing injection midbody and a finish product. The invention is characterized in that each index component content is measured by adopting the high performance liquid chromatography or other analysis methods; the diluted sample is scanned in full wavelength by adopting the ultraviolet spectrophotometric method; the sample is divided into two parts: a corrected collection and a verified collection. According to the ultraviolet spectroscopic data and the content of the corrected collection, the data are processed by adopting the chemometrics method; a mathematical model is established by utilizing a TQ Analyst software and a Matlab 6.5 software; the ultraviolet spectroscopic data of the verified collection sample is substituted into the mathematical model; a predicted value and a true value are compared, and the precision and the reliability of the technology are verified. The method of the invention can be used for content measurement of chlorogenic acid and baicalin in the silver yellow liquid, and the content measurement of Caryptoside and total nitrogen in the four-miscible liquid. The invention also can be used for the fast online detection of component content hereinbefore, and can be used for the content measurement and fast online detection of the Qingkailing injection midbody and the finish product.
Owner:BEIJING UNIV OF CHINESE MEDICINE

Rapid algae-liquid separation method for microalgae treatment wastewater

The invention discloses a rapid algae-liquid separation method for microalgae treatment wastewater. The method includes the steps of collecting wastewater discharged by a sewage treatment plant to be put indoors for standby application, processing CODCr of wastewater through the potassium dichromate method, processing TN through the potassium persulfate oxidation ultraviolet spectrophotometry, processing TP through the antimony and molybdenum spectrophotometry, processing ammonia nitrogen through the Nessler spectrophotometry, and adjusting the pH through the glass electrode method. The method can be conducted in a cultivation device with the controllable turbulence intensity, temperature and light can be conveniently controlled, the efficiency of microalgae for processing wastewater is improved, no flocculant needs to be added under the turbulence condition, flocculating constituent is formed according to the property changes of microalgae cell surfaces so that algae and liquid can be separated, turbulence energy consumption can be sufficiently utilized, energy consumption is lowered, efficiency cost is improved, the pH of a culture solution is increased during processing, pathogenic organisms in a culture medium can be restrained, the produced flocculating constituent has high sedimentation speed, the algae-liquid separation efficiency is improved, operation is easy and convenient, cultivation conditions are easy to control, and an experimental device for the method is simple in structure, small in size, light in weight, high in practicability and repeatability, simple in process, convenient to machine, capable of saving labor, materials and financial resources, flexible to operate and convenient to clean.
Owner:JIANGXI NORMAL UNIV

Pediatric paracetamol, cow-bezoar and chlorphenamine maleate granules and quality control method thereof

The invention discloses pediatric paracetamol, cow-bezoar and chlorphenamine maleate granules and a quality control method thereof. The granules are prepared from paracetamol, in-vitro cultivated cow-bezoar and chlorphenamine maleate in a ratio of 125:5:0.5. The quality control method combines identification, thin-layer chromatography, thin-layer scanning, and ultraviolet spectroscopy. In the granules, in-vitro cultivated cow-bezoar is used to replace natural cow-bezoar, so as to greatly reduce raw material cost of medicine while ensuring pesticide effect. In addition, the replacement of in-vitro cultivated cow-bezoar for artificial cow-bezoar so that the medicament safety and pesticide effect problems can be solved, and is favorable for comprehensive quality control of the pediatric paracetamol, cow-bezoar and chlorphenamine maleate granules.
Owner:文永盛 +2

Silver-carbon quantum dots compound and preparation method and application thereof

The invention belongs to the technical field of carbon nanomaterials and especially relates to a silver-carbon quantum dots compound and a preparation method and application thereof. The silver-carbonquantum dots compound comprises carbon quantum dots and silver loaded on the surface of the carbon quantum dots. The carbon quantum dots are obtained through carbonization of squid ink. The silver-carbon quantum dots compound can be used as a catalyst for catalyzing p-nitrophenol reduction reaction. By ultraviolet spectrophotometry for detecting catalytic action of the silver-carbon quantum dotscompound on p-nitrophenol reduction reaction, results show that the silver-carbon quantum dots compound has fast catalytic speed of reduction reaction. Dosage of the silver-carbon quantum dots compound as a catalyst is less, and an efficient catalyst is provided for the catalytic reduction reaction of p-nitrophenol.
Owner:GUANGDONG UNIV OF TECH

Preparation of loquat leaf total flavones and functions thereof for reducing blood sugar and blood fat

The invention relates to a method for preparing folium eriobotryae total flavonoids and hypoglycemic and antilipemic functions, and provides a method for extracting and preparing the total flavonoids from folium eriobotryae and functions and use of the total flavonoids prepared by the method. Firstly, the method for preparing the folium eriobotryae total flavonoids is as follows: folium eriobotryae medicinal materials undergo reflux extraction, soaking extraction or percolation extraction through alcohols or methanol; extracting solution is decompressed, concentrated, precipitated after addition of water and then centrifuged; macroporous resins or polyamide columns are added into supernatant; after elution of impurities by water, gradient elution of the supernatant is performed by alcohols; alcohol eluent is collected, decompressed, concentrated, dried and crushed, and then folium eriobotryae total flavonoid extract is obtained. Secondly, the quality standard of the folium eriobotryae total flavonoids is as follows: the ultraviolet spectrophotometric method is used for determination; NaNO2-Al (NO3) 3-NaOH is used for color development; and rutoside is used for calculation, the total content of the flavonoids is not lower than 50 percent. Thirdly, the functions of the folium eriobotryae total flavonoids are as follows: the functions are hypoglycemic and antilipemic functions, and the use is to cure diabetes and blood-fat metabolic disturbance caused by the diabetes.
Owner:INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI

UV spectrophotometry and HPLC combined method for determining content of phenylpropanol

The invention relates to detecting the alcohol-within-benzene content jointly using UV spectrophotometry method and HPLC method, and the method is aimed at the quality control to the phenylpropanol content and impurities of the drug; the quality control method of the phenylpropanol and its preparation in the invention includes the content detection method of phenylpropanol and effective phenylpropanol component of preparation, and the impurities content detection method of phenylpropanol and its preparation; among them, the content detection method of phenylpropanol and effective phenylpropanol component of preparation in the invention has the following steps: a. the preparation of standard solution, b. the preparation of the testing solution, c. standard curve mapping, d. sample content detection using UV spectrophotometry, with 60:40 phosphate water buffer solution as the solvent, at 257nm wavelength to measure absorbance, and according to the absorbance calculating the sample content.
Owner:NANJING NORMAL UNIVERSITY +1

Shuteria involucrata root extract as well as preparation method and application thereof

InactiveCN105380983AStable in natureSignificant antitussive and expectorant activityRespiratory disorderPlant ingredientsChinese traditionalObstructive chronic bronchitis
The invention relates to a shuteria involucrata root extract as well as a preparation method and application thereof and belongs to the field of modernization of Chinese traditional medicines. The shuteria involucrata root extract is prepared by the following steps: taking shuteria involucrata roots as raw materials, carrying out ethanol water extraction and recycling a solvent to obtain a total extract; after water dispersion, centrifuging the total extract; degreasing the centrifuged liquid with petroleum ether and then extracting with ethyl acetate; recycling an extracting agent to obtain an ethyl acetate extract; after diluting the extract, carrying out column chromatography separation; determining and collecting a flavone part through a thin layer chromatography analysis and ultraviolet spectrophotometry, and concentrating the flavone part to obtain the extract. The research finds that the shuteria involucrata root extract has remarkable activity for relieving a cough and eliminating phlegm and particularly has an excellent phlegm eliminating effect when the general flavone part is in a low dosage; the shuteria involucrata root extract is safe and effective and can be used for preparing medicines for preventing and treating a cold and chronic bronchitis.
Owner:YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE

Instrument measuring method for total nitrogen in coking wastewater

The invention discloses an instrument measuring method for total nitrogen in coking wastewater, which relates to an analytic measuring method for chemical substances, and solves the problems of operation complication and time waste of total nitrogen measurement of an existing potassium persulfate oxygenation-ultraviolet spectrophotometric method. The measuring method provided by the invention utilizes a TOC (total organic carbon) analyzer to measure the total nitrogen in the coking wastewater, and comprises the steps that: a standard solution is firstly prepared and a standard curve of the standard solution is established, the total nitrogen and an ozone gas source in a sample are combined and then resolved into nitrogen monoxide after the sample is combusted, the nitrogen monoxide gas is driven by carrier gas to enter into a chemiluminescence detector to be detected, and then the total nitrogen concentration in the sample is measured by comparing a detecting signal sent from the detector with the standard curve. Compared with the potassium persulfate oxygenation-ultraviolet spectrophotometric method, the measuring method provided by the invention has the obvious advantages that the operation is simple, and the consuming time is only about 15 minutes.
Owner:宝钢化工湛江有限公司

Determination method of total 2-(2-phenethyl) chromone compound content

The invention relates to a determination method of a total 2-(2-phenethyl) chromone compound content, comprising the following steps of: extracting and filtering an agilawood sample to obtain a solution to be determined; by taking 6, 7-dimethoxy-2(2-phenethyl) chromone as a reference substance, dissolving the solution to be determined with a same solvent, preparing standard solutions with different concentrations, determining a light absorption value under 230nm, and establishing a standard curve; and determining the light absorption value under 230nm after the solution to be determined is diluted, the total 2-(2-phenethyl) chromone compound content in the solution to be determined is calculated by the standard curve and a dilution multiple, and finally calculating the total 2-(2-phenethyl) chromone compound content in the agilawood sample. According to the determination method disclosed by the invention, operation is convenient; and by utilizing the characteristics that a 2-(2-phenethyl) chromone compound has a 2-(2-phenethyl) chromone mother nucleus and determining the total 2-(2-phenethyl) chromone compound content in an agilawood substance by adopting an ultraviolet spectrophotometric method, pertinence is strong, stability is good, accuracy is high, and repeatability is good.
Owner:厦门名香生物科技有限公司

Method for measuring minimum inhibiting concentration of heavy metal to microorganism

The invention provides a method for quickly measuring the minimum inhibitory concentration of heavy metals on microbe, comprising: respectively preparing liquid culture medium adding with heavy metal ion gradient concentration which is suitable for growth of the microbe, moving strain in logarithmic phase to sterilized culture medium for culture in sterile conditions, measuring OD value by using UV spectrophotometry, and obtaining the minimum inhibitory concentration which has no increase change in OD values measured several times. The method is favorable to quickly select strains, and is more reliable for measuring the inhibitory of heavy metals on microbe.
Owner:HUNAN UNIV

Biomarker for predicting II-type diabetic vascular complication risk and application thereof

The invention belongs to the technical field of biology and diagnosis, and relates to a receptor of a definite, effective and visualized platelet reactivity biomarker P2Y12 and an application thereof for diagnosing and predicting the II-type diabetic vascular complication risk. The platelet activation biomarker P2Y12 can detect expression levels of platelet through fluorescent quantitative polymerase chain reaction (PCR), Western blot, enzyme linked immunosorbent assay (ELISA), flow cytometry, UV spectrophotometry-near infrared spectrometry, high performance liquid chromatography, colorimetry, a mass spectrum identification method and the like and detect content of P2Y12 in whole blood. The invention also provides a method of biomarker P2Y12 for diagnosing and predicting vascular complication of subjects as well as a medicine for treating II-type diabetic vascular complication and a screening method of the medicine. The invention further provides an intervene plane for individualizing P2Y12 inverse agonist and accurately treating the vascular complication of II-type diabetic patients.
Owner:FUDAN UNIV

Method for measuring content of humic acid in oil-containing sludge in oilfield

The invention discloses a method for measuring the content of humic acid in oil-containing sludge in oilfield in the sludge treatment field. The method comprises the following steps: 1, preparing a sludge sample; 2, preparing a solution to be measured; 3, drawing a standard curve: preparing multiple sodium humate standard solutions in volumetric flasks, adding a sodium hydroxide solution to scale lines, sequentially carrying out wavelength scanning on the solutions in the volumetric flasks by using an ultraviolet spectrophotometer in the order of the concentrations of the solutions in order to obtain maximum absorbance values corresponding to maximum absorption peaks, recording the maximum absorbance values, drawing the standard curve, and calculating a linear equation; and 4, measuring the concentration of the sample: diluting the solution to be measured, placing the diluted solution to be measured in the ultraviolet spectrophotometer, detecting the absorbance of the solution to be measured, and calculating the content of the humic acid by using the linear equation in step 3. The method adopting ultraviolet spectrophotometry has the advantages of short time, simplicity in operation, and high precision, and can be used in the measurement of the humic acid in the oil-containing sludge in the oilfield.
Owner:CHINA PETROLEUM & CHEM CORP +1

Method for measuring tannin extract content in tannin extract doctor solution

The present invention belongs to chemical technology, and is ultraviolet spectrophotometric method of measuring the content of tanning extract in tanning extract desulfurizing liquid. The method has the materials including industrial tanning extract, sodium carbonate, sodium bicarbonate and sodium metasilicate; and adopts ultraviolet / visible light spectrophotometer. The measuring process includes regulating the measurement wavelength to 320 nm, regulating pH value and other technological parameters, diluting the solution of tanning extract properly, drawing standard curve and forming regression formula, measuring the absorbance of the measured liquid and finding out the content of tanning extract based on the standard curve. The method is accurate.
Owner:TAIYUAN UNIV OF TECH

Rapid salicylate spectrophotometric assay method for ammonia nitrogen in water

The invention discloses a rapid salicylate spectrophotometric assay method for ammonia nitrogen in water. The rapid salicylate spectrophotometric assay method is characterized in that a centrifuge is utilized for centrifugal separation of a water sample subjected to flocculation and sedimentation to remove flocculent precipitates in the water sample, then water-bath heating is utilized to improve the chemical equilibrium constants of ammonia nitrogen reaction, and finally, the ultraviolet spectrophotometry is adopted for detection. The rapid salicylate spectrophotometric assay method speeds up reaction, reduces interference of flocculates, enables follow-up detection to be accurate and reliable, is high in flexibility and excellent in recovery rate and precision, and therefore, can finish detection more quickly on the premise of guaranteeing the accuracy.
Owner:淮安自来水有限公司

Serum monoamine oxidase detection kit

The invention relates to the technical field of the clinical in-vitro detection and discloses a serum monoamine oxidase detection kit. The serum monoamine oxidase detection kit is characterized in that a reagent is a single reagent and can be used and operated conveniently compared with dual reagents on a full-automatic biochemical analysis instrument. The ultraviolet spectrophotometry is adopted to test the content of monoamine oxidase in serum; compared with the domestic common kit, the kit adopts new buffer solution and stabilizers, the stability of the reagent is improved remarkably and the detection accuracy is ensured; a coenzyme NADH (Nicotinamide Adenine Dinucleotide Hydrogen) regeneration system is adopted, a reaction substrate NADH can keep the stability all the time in a reagent storage process under the participation of enough glucose dehydrogenase, and the reagent stability and the detection accuracy are improved remarkably; novel ampholytic surfactant-dodecyl dimethyl betaine is adopted, the determination performance is improved remarkably, and the reagent stability and the anti-jamming capability are improved remarkably.
Owner:BIOBASE BIODUSTRY (SHANDONG) CO LTD

Method for preparing total flavonoids of herba Euphorbiae Flavescentis

A method for preparing total flavonoids of herba Euphorbiae Flavescentis belong to the field of effective parts of traditional Chinese medicine. The preparation method comprises the following steps: (1) roughly extracting the herba Euphorbiae Crataegi with 55%-75% ethanol was extract by heating and reflux; 2, refine: adopting polyamide column chromatography, elute with water, 15% ethanol and 75% ethanol in turn, collecting 75% eluent, concentrating unde reduced pressure, and drying to obtain total flavonoids of herba Euphorbiae Humifusae; (3) determination of total flavonoids content: the content of total flavonoids was determined by UV spectrophotometry. The content of total flavonoids was more than 50% according to arborvitae. The content of total flavonoids of herba cynanchi paniculatimeets the requirements of the effective part preparation of traditional Chinese medicine, and can be used as the raw material medicine of the traditional Chinese medicine preparation.
Owner:SOUTHWEST MEDICAL UNIVERISTY +1

Method for determining content of main active components in Alpinia katsumadai by using ultraviolet spectrophotometry

The invention discloses a method for determining the content of main active components in Alpinia katsumadai by using ultraviolet spectrophotometry. The method uses ultrasonic waves as an auxiliary tool, and comprises the following steps: sampling, preparing a sample, carrying out solvent and ultrasonic wave assisted extraction, carrying out ultraviolet detection, and calculating the content of the main active components. The method allows the main active components of Alpinia katsumadai to be completely and rapidly extracted and to be determined. The method applying an Alpinia katsumadai ultraviolet spectrophotometry testing system realizes rapid and quantitative detection of the concentrations of main active components comprising alpinetin and cardamonin in the Alpinia katsumadai. The method has the advantages of simple operation, accurate determination result, and difficult interference by other substance components.
Owner:HAINAN NORMAL UNIV

Method of measuring activity of scutellaria baicalensis endogenous beta-D-glucuronidase

The invention discloses a method of measuring activity of scutellaria baicalensis endogenous beta-D-glucuronidase. The method comprises following steps of (1) preparing a buffer, a sodium carbonate solution and a paranitrophenol standard solution; (2) extracting scutellaria baicalensis crude enzyme; (3) drawing a standard curve with concentration of paranitrophenol as a horizontal axis and absorbance at absorbing peak as a longitudinal axis; (4) mixing the buffer, the crude enzyme and P-nitrobenzene-beta-D-glucuronide solution, heating and reacting, after reaction, and measuring absorbance ofa reaction solution by using a UV spectrophotometer; and (5) substituting obtained absorbance through measurement into the standard curve, calculating molar concentration of paranitrophenol produced in reaction, and calculating activity of beta-D-glucuronidase by using a formula. The method measures quantity of paranitrophenol hydrolyzed from a certain amount of substrate by beta-D-glucuronidase within a certain time period by using UV spectrophotometry, so as to determine enzyme activity.
Owner:HUAIYIN INSTITUTE OF TECHNOLOGY

Day lily flower and method for detecting day lily flower preparation

The invention discloses day lily flower and a method for detecting a day lily flower preparation. The method for detecting the day lily flower preparation comprises one or more the following identification methods and / or content measurement methods of: A, thin-layer chromatography identification; B, ultraviolet spectrophotometry content measurement; C, HPLC (High Performance Liquid Chromatography) content measurement; and D, HPLC fingerprint chromatography of an original medicinal material. The invention also further provides an HPLC detection method and an HPLC fingerprint chromatography detection method of the day lily flower. According to the day lily flower and the method for detecting the day lily flower preparation, which are disclosed by the invention, the identification specificity is good and the method is low in cost and is practical and is rapid in obtaining a result; the product can be effectively subjected to quality detection by the content measurement methods; and an analysis method in the fingerprint chromatography detection is stable, reliable and effective and the basis is provided for identification and quality evaluation and control of the day lily flower preparation and the medicinal material.
Owner:倪健
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