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Method for determining content of main active components in Alpinia katsumadai by using ultraviolet spectrophotometry

A technology of spectrophotometry and active ingredients, which is applied in the field of analytical chemistry, can solve problems such as impurity interference, long time, and long separation time, and achieve good precision, accurate results, and simple operation.

Active Publication Date: 2016-02-17
HAINAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among these methods, the operating conditions of high performance liquid chromatography are not easy to control and are more cumbersome, and the time is too long
The amount of sample used in thin-layer chromatography is large, the separation procedures are troublesome, the separation time is long, and there may be interference from impurities
However, micellar electrokinetic capillary chromatography is prone to bubbles, easily broken columns, low detection sensitivity, and limited types of fillers, etc.

Method used

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  • Method for determining content of main active components in Alpinia katsumadai by using ultraviolet spectrophotometry
  • Method for determining content of main active components in Alpinia katsumadai by using ultraviolet spectrophotometry
  • Method for determining content of main active components in Alpinia katsumadai by using ultraviolet spectrophotometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Establishment of the Linear Regression Equation of Alpinin Standard Curve

[0064] (1) Preparation of alpinol standard solution: Accurately weigh 0.0135g of alpinin, and prepare them with 95% ethanol to a concentration of 1.0×10 -3 mol / L standard sample solution. Dilute to appropriate different concentrations when used;

[0065] (2) Obtaining the linear regression equation of the standard curve: the standard sample solutions with different concentrations were measured by ultraviolet spectrophotometry, and the detection wavelength was 286nm for alpinol, and the obtained absorbance was drawn into a standard curve, and according to Lambert-Beer's law calculation to obtain the standard curve linear regression equation (such as figure 1 shown).

Embodiment 2

[0067] Establishment of Cardamonin Standard Curve Linear Regression Equation

[0068] (1) Preparation of cardamonin standard solution: Accurately weigh 0.0135g of cardamonin, and prepare them with 95% ethanol to a concentration of 1.0×10 -3 mol / L standard sample solution. Dilute to appropriate different concentrations when used;

[0069] (2) Obtaining the linear regression equation of the standard curve: the standard sample solutions with different concentrations were measured by ultraviolet spectrophotometry, and the detection wavelength was 346nm for cardamomine, and the obtained absorbance was drawn into a standard curve, and according to Lambert-Beer's law calculation to obtain the standard curve linear regression equation (such as figure 2 shown).

Embodiment 3

[0071] Establishment of a method for the determination of alpinin and cardamomin in bawanglingcao and bawanglingcao cardamom by ultraviolet spectroscopy

[0072] 1. Prepare the samples to be tested: Take the samples of cardamom from Baisha grass and cardamom from Bawangling grass from the refrigerator at -80 degrees, peel the skins, leave the seed balls, dry them in an oven at 60 degrees Celsius, and then crush them with a grinder and pass through them for 50 Mesh sieve, accurately weigh 5g of white sand grass cardamom and Bawangling grass cardamom powder, add 95% ethanol to the volume of 100mL in the reagent bottle, ultrasonically dissolve for 40min, then place it overnight, filter, and take the subsequent filtrate to pass through a 0.25μm microporous membrane , the final filtrate is used for splitting equipment;

[0073] 2. Determination of test sample: each part of sample to be tested absorbs 10 μ L and settles to 3mL respectively again, then transfers in the quartz cuvette...

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Abstract

The invention discloses a method for determining the content of main active components in Alpinia katsumadai by using ultraviolet spectrophotometry. The method uses ultrasonic waves as an auxiliary tool, and comprises the following steps: sampling, preparing a sample, carrying out solvent and ultrasonic wave assisted extraction, carrying out ultraviolet detection, and calculating the content of the main active components. The method allows the main active components of Alpinia katsumadai to be completely and rapidly extracted and to be determined. The method applying an Alpinia katsumadai ultraviolet spectrophotometry testing system realizes rapid and quantitative detection of the concentrations of main active components comprising alpinetin and cardamonin in the Alpinia katsumadai. The method has the advantages of simple operation, accurate determination result, and difficult interference by other substance components.

Description

technical field [0001] The invention relates to a method for determining the content of main active components of cardamom, belonging to the field of analytical chemistry, in particular to a related technology for determining the content of main active components of cardamom by ultraviolet spectroscopy. Background technique [0002] Grasshopper is the dry seed mass of Alpiniakatsumadai Hayata, a plant belonging to the genus Zingiberaceae. It is a commonly used traditional Chinese medicine, mainly produced in Guangdong, Guangxi and Yunnan. It has the functions of drying dampness, strengthening the spleen, warming the stomach and relieving vomiting. Its function is mainly used to treat diseases such as cold-damp internal resistance, fullness and cold pain in the dirty abdomen, vomiting due to heating, and lack of appetite. Cardamom is one of the varieties of Dai medicine, and its rhizome can cure various aches and pains caused by rheumatism. The extract of cardamom has obviou...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N1/28
Inventor 何文英陈昱学吴秀丽吴禄勇
Owner HAINAN NORMAL UNIV
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