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Method of detecting contents of cellulose, hemicellulose and lignin of corn stovers

A technology of corn stalks and hemicellulose, which is applied in the field of agriculture, can solve problems such as roughness, and achieve the effect of simple method, high accuracy and easy operation

Inactive Publication Date: 2013-07-10
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of these methods are relatively rough, and there will be large errors in the experimental process. Therefore, this method can greatly reduce the errors in the test and achieve accurate testing.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] This embodiment relates to a method for testing corn stalk cellulose, hemicellulose and lignin content, said method comprising the following steps:

[0025] Using Shanghai corn as raw material, use this method to test cellulose, hemicellulose and lignin:

[0026] The first step, raw material preparation: select about 10 corn stalks from a certain corn field in Qili Village, Yangang Town, Chongming County, Shanghai, using the scientific distribution method, peel off the stems and leaves, air-dry them, and then crush them through a 40-mesh sieve;

[0027] The second step is the test of dry matter: dry the crushed and sieved corn stalks at 105°C, and record the dry matter amount of corn stalks at 105°C, which is 908.57g / kg;

[0028] The third step is the test of acid soluble lignin: take a certain amount of crushed and sieved corn stalks, carry out acid hydrolysis and autoclave sterilization, cool to room temperature and filter the supernatant for UV spectrophotometry, and...

Embodiment 2

[0034] This embodiment relates to a method for testing corn stalk cellulose, hemicellulose and lignin content, said method comprising the following steps:

[0035] Using corn in Jiangxi as raw material, use this method to test cellulose, hemicellulose and lignin:

[0036] The first step, raw material preparation: select about 10 corn stalks from a certain corn field in Yichun City, Jiangxi Province by the scientific distribution method, peel off the stems and leaves, air-dry them, and then crush them through a 60-mesh sieve;

[0037] The second step is the test of dry matter: dry the crushed and sieved corn stalks at 105°C, and record the dry matter amount of corn stalks at 105°C, which is 942.04g / kg;

[0038] The third step is the test of acid soluble lignin: take a certain amount of crushed and sieved corn stalks, carry out acid hydrolysis and autoclave sterilization, cool to room temperature and filter the supernatant for UV spectrophotometry, and calculate the acidity acco...

Embodiment 3

[0044] This embodiment relates to a method for testing corn stalk cellulose, hemicellulose and lignin content, said method comprising the following steps:

[0045] Using corn in Anhui as raw material, use this method to test cellulose, hemicellulose and lignin:

[0046] The first step, raw material preparation: select about 10 corn stalks in a certain corn field in Mengcheng County, Anhui Province by scientific distribution method, peel off the stems and leaves, air-dry them, and then crush them through a 40-mesh sieve;

[0047] The second step is the test of dry matter: dry the crushed and sieved corn stalks at 105°C, and record the dry matter amount of corn stalks at 105°C, which is 964.61g / kg;

[0048] The third step is the test of acid soluble lignin: take a certain amount of crushed and sieved corn stalks, carry out acid hydrolysis and autoclave sterilization, cool to room temperature and filter the supernatant for UV spectrophotometry, and calculate the acidity according...

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Abstract

The invention relates to a method of detecting contents of cellulose, hemicellulose and lignin of corn stovers. The method comprises: 1, taking treated corn stovers, crushing, and drying to obtain the corn stover powder; 2, detecting the content of the lignin of the corn stover powder; and 3, detecting the contents of the hemicellulose and the lignin of the corn stover powder. The method combines a UV spectrophotometry method and an HPLC method to detect the contents of the cellulose, the hemicellulose and the lignin of the corn stovers, is simple, easy to operate and high in accuracy, and has a positive significance for quantification of biomass energy use and development of related equipment.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a method for testing the contents of cellulose, hemicellulose and lignin in corn stalks. Background technique [0002] Corn is a crop that is mainly used as feed for grain, economics and fodder, and corn stalks are also an important production resource for industrial and agricultural production. As a resource, corn stover is rich in nutrients and available chemical components, which can be used as raw material for livestock feed, and its high fiber content can be used as raw material for bioenergy (such as fuel ethanol, bio-oil). [0003] The content of cellulose, hemicellulose and lignin in biomass directly affects the conversion rate of biomass energy. Therefore, as a common biomass, corn stalks can accurately test the cellulose, hemicellulose and lignin in corn stalks. The content of lignin is very important for the quantitative process of developing biomass. [0004] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N5/04G01N21/33G01N30/02
Inventor 刘荣厚王璐孙辰蔡文飞梅远飞尹仁湛
Owner SHANGHAI JIAO TONG UNIV
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