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Quality controlling method of ganoderma aqueous extract

A quality control method and water extract technology, applied in the field of quality control of Ganoderma lucidum water extract, can solve the problem of high determination results of Ganoderma lucidum polysaccharides

Active Publication Date: 2012-10-17
福建省食品药品质量检验研究院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If it is determined according to the method of the Chinese Pharmacopoeia of the 2010 edition, the determination result of Ganoderma lucidum polysaccharide will be too high

Method used

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  • Quality controlling method of ganoderma aqueous extract
  • Quality controlling method of ganoderma aqueous extract
  • Quality controlling method of ganoderma aqueous extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 Determination of crude polysaccharide content

[0038] A1.1 Principle

[0039] The relative molecular mass in the sample is greater than 1×10 4 Precipitate high molecular substances in 80% ethanol solution, separate from monosaccharides and oligosaccharides in aqueous solution, use alkaline divalent copper reagent to selectively precipitate polysaccharides with dextran structure from other high molecular substances, and use phenol - Sulfuric acid reacts to generate an orange-red compound, and its content is determined by colorimetry, and its color intensity is proportional to the content of dextran in the crude polysaccharide, so as to calculate the content of the crude polysaccharide in this product.

[0040] A1.2 Reagents

[0041] A1.2.1 Reagents and materials

[0042] A1.2.1.1 Ethanol: analytically pure.

[0043] A1.2.1.2 Concentrated sulfuric acid: analytically pure.

[0044] A1.2.1.3 Sodium hydroxide solution (100 g / L): Weigh 25 g of sodium hydro...

Embodiment 2

[0074] Example 2 Ganoderma acid A, B, C 2 Identification and Quantitative Determination

[0075] A2.1 Principle

[0076] The sample was extracted with methanol as a solvent, ultrasonically extracted, and Ganoderma acid A and Ganoderma acid B were passed through a high-performance liquid chromatography column (C 8 ) separation; Ganoderma acid C 2 After high performance liquid chromatography column (C 18 ) separation, detected by ultraviolet detector (UV), respectively, according to the retention time of the chromatographic peak, and the external standard method of the peak area for quantification, respectively determine the ganoderma acid A, B and ganoderma acid C in the sample 2 content and calculate its total amount.

[0077] A2.2 Reagents

[0078] A2.2.1 Water: Purified water.

[0079] A2.2.2 Acetonitrile: chromatographically pure.

[0080] A2.2.3 Glacial acetic acid: analytically pure.

[0081] A2.2.4 Methanol: analytically pure.

[0082] A2.2.5 Ganoderma acid A...

Embodiment 3

[0148] The index of symbolic components shall meet the requirements in Table 3.

[0149] Table 3 Indicators of iconic components

[0150]

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PUM

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Abstract

The invention relates to a quality analysis analysis method of medicines or health care products, and specifically relates to a quality controlling method of a ganoderma aqueous extract. For controlling the product quality with high specificity, according to the invention, crude polysaccharide contents and a sum of ganoderma acid A, B, and C2 contents are adopted as quality controlling indexes of the ganoderma aqueous extract. According to the invention, ganoderma crude polysaccharide is extracted with a water extraction and alcohol precipitation method; polysaccharide with a glucan structure is precipitated by using a copper reagent; and the content of the polysaccharide is determined by using an ultraviolet spectrophotometry method. Therefore, the influences of auxiliary materials on determination are eliminated, and a basis is provided for quality controlling of the ganoderma aqueous extract. The contents of the characteristic components ganoderma acid A, B, and C2 in ganoderma lucidum, ganoderma sinnese, and ganoderma tsugae are relatively high. Compared to a currently commonly-used colorimetric method for determining total triterpenoid contents, the specificity of the method provided by the invention is high. With the method, purposes of authentication and quality control can be achieved.

Description

technical field [0001] The invention relates to a method for analyzing the quality of medicines or health care products, in particular to a method for controlling the quality of ganoderma lucidum water extract. Background technique [0002] Ganoderma lucidum Ganoderma lucidum (Leyss. ex Fr.) Karst. or Zizhi Ganoderma sinense Zhao, Xu et Zhang or Ganoderma lucidum Ganoderma The dry fruiting body of tsugae Murr. has the effects of invigorating qi and calming the nerves, relieving cough and relieving asthma. The Ganoderma lucidum extraction process mainly includes water extraction and alcohol extraction, and most of them use water extraction process, that is, the Polyporaceae fungus Chizhi ( Ganoderma lucidum (Leyss. ex Fr.) Karst.), Zizhi ( Ganoderma sinnese Zhao, Xu et Zhang) and Ganoderma lucidum ( Ganoderma tsugae Murr.) obtained by water extraction, concentration and drying. Therefore, the present invention adopts the water extract of Ganoderma lucidum to na...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N21/31
Inventor 王勇陈硕卢端萍蒋婷婷范明李晔吴长辉
Owner 福建省食品药品质量检验研究院
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