The invention discloses a pharmaceutical composition used for treating virus hepatitis, a preparation method and a quality control method thereof. The pharmaceutical composition has the raw materials of herba artemisiae capillaris, radix isatidis, rhizoma dryopteris crassirhizomae, indian bread, radix curcumae, herba scutellariae barbatae and patchouli, etc. The quality control method is that thetraditional Chinese medicine provided by the invention is obtained by a great deal of creative experiments and screening as well as by the screening for a sample treatment method and the selection toa developing agent in a distinguishing method, and the distinguishing specificity is good; furthermore, the methods are economical and applicable, has rapid result and can be applied to different thin-layer plates. In a content measuring method, by the screening for a processing method of samples and test product and the selection to the developing agent, the content measuring method can effectively carry out quality control on products; furthermore, compared with other methods, the product measured by the method has more stable drug effect.