The invention provides preserving fluid of hepatic cells for a biological
artificial liver and a preparation method thereof. The preserving fluid is a solution compounded by
ultrapure water. The solution contains the following components within the concentration range: 15-25mmol / L of
disodium hydrogen phosphate, 1-10mmol / L of
sodium hydrogen phosphate dehydrate, 4-6mmol / L of
potassium citrate monohydrate, 10-30mmol / L of
sodium chloride, 5-10mmol / L of
magnesium chloride hexahydrate, 3-10mmol / L of disodium
adenosine triphosphate, 1-5mmol / L of reducing
glutathione, 0.1-0.5mmol / L of alpha-
lipoic acid, 100-150mmol / L of
trehalose (C6H12O5), 200 / 0.510-50g / L of
hydroxyethyl starch and 2-10mg / L of
matrine. The preparation method of the preserving fluid comprises the following steps of: accurately weighing all components according to the concentration requirements of the components, wherein the alpha-
lipoic acid is weighed in a dark place; completely dissolving the other components except the alpha-
lipoic acid by using the right amount of
ultrapure water; sufficiently dissolving the alpha-lipoic acid in the dark place; and adding the
ultrapure water to full
dose. The preserving fluid can well protect the
cell activity of the hepatic cells for the biological
artificial liver and the special functions of the hepatic cells at low temperature so as to satisfy the short-term low temperature preservation of a large-scale hepatic
cell bank for the biological
artificial liver and / or the hepatic
cell protection in the long-distance transportation process.