Method for preparing stable albumin nano-particles

A technology of albumin nanometer and albumin, which is applied in the field of preparation of albumin nanoparticle, can solve the problems of difficult in vivo delivery of pharmacologically active substances and the like

Active Publication Date: 2013-07-24
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for preparing stable albumin nanoparticles, which are stabilized by cross-linking intermolecular disulfide bonds, so as to solve the problem that certain pharmacologically active substances are difficult to deliver in vivo

Method used

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  • Method for preparing stable albumin nano-particles
  • Method for preparing stable albumin nano-particles
  • Method for preparing stable albumin nano-particles

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Example 1, preparation of disulfide bond-stabilized human serum albumin nanoparticles.

[0038] Using human serum albumin and reduced glutathione as raw materials, disulfide bond-stabilized human serum albumin nanoparticles were prepared.

[0039] The specific preparation method is as follows:

[0040]Dissolve 20 mg of human serum albumin in 1 mL of aqueous solution, add 20 mM reduced glutathione, react at room temperature for 1 hour, then add 4 mL of absolute ethanol to react for 30 minutes, and then dialyze the prepared suspension bag, dialyzed in deionized water at 4°C for 24 hours to obtain disulfide bond-stabilized human serum albumin nanoparticles, and the average particle size of albumin nanoparticles is 100-300nm (ZetaPALS, Zeta Potential Analyzer ).

[0041] The stability of the prepared nanoparticles in different solvent environments can be obtained from figure 1 seen in. The stepwise dissolution process of the as-prepared nanoparticles under reducing envi...

Embodiment 2

[0042] Example 2, preparation of disulfide bond-stabilized human serum albumin nanoparticles.

[0043] Using human serum albumin and reduced glutathione as raw materials, disulfide bond-stabilized human serum albumin nanoparticles were prepared.

[0044] The specific preparation method is as follows:

[0045] Dissolve 200 mg of human serum albumin in 1 mL of aqueous solution, add 500 mM reduced glutathione, react at 50°C for 10 minutes, then add 1 mL of absolute ethanol for 5 minutes, and then dialyze the obtained suspension The bag is dialyzed in deionized water at 10° C. for 24 hours to obtain disulfide bond-stabilized human serum albumin nanoparticles, and the average particle size of the albumin nanoparticles is 100-400 nm.

Embodiment 3

[0046] Example 3, preparing a disulfide bond-stabilized curcumin-human serum albumin nanoparticle drug-loading system.

[0047] Human serum albumin, reduced glutathione and curcumin were used as raw materials to prepare a drug-carrying nanoparticle system of curcumin coated with human serum albumin stabilized by disulfide bonds.

[0048] The specific preparation method is as follows:

[0049] Dissolve 40 mg of human serum albumin in 1 mL of aqueous solution, add 50 mM reduced glutathione, react at room temperature for 1 hour, then add 4 mL of 3 mg / mL curcumin ethanol solution and react for 30 minutes, then prepare the obtained The suspension was placed in a dialysis bag, and dialyzed in deionized water at 4° C. for 24 hours to obtain disulfide bond-stabilized curcumin-human serum albumin-coated nanoparticles. Moreover, the average particle size of nanoparticles is 50-400nm (ZetaPALS, Zeta Potential Analyzer), and the drug-loading capacity of curcumin is determined by reversed...

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Abstract

The invention discloses a method for preparing stable albumin nano-particles, belonging to the technical field of biological medicine material preparation. The method comprises the following steps of: carrying out pretreatment on albumin by utilizing non-biotoxic glutathione or cysteine to open intramolecular dithio bonds, precipitating the albumin by using anti-solvents such as alcohol and the like, and carrying out sulfydryl-dithio bond exchange reaction so as to obtain albumin nano-particles with intermolecular dithio bonds. The prepared nano-particles can be used for delivering in-vivo pharmacologically active substances and / or diagnosis aids. The obtained albumin nano-particles have the advantages that the albumin nano-particles not only have good stability under dilution condition, but also have oxidation reduction response in a reduction type environment; and due to such characteristics, the particles can stably exist in in-vivo blood circulation systems of organisms and are degraded under the action of reduced glutathione so as to release the wrapped medicine.

Description

technical field [0001] The invention relates to a method for preparing albumin nanoparticles, belonging to the technical field of preparation of biomedical materials. Background technique: [0002] Albumin is a biological endogenous protein with many characteristics such as biodegradability, non-toxicity, and non-antigenicity. It is considered to be an ideal drug carrier. research direction. Albumin drug-loaded systems are mainly divided into two categories, one is chemically coupled albumin drug-loaded systems, and the other is physically bound albumin drug-loaded systems. Chemically conjugated albumin can improve the pharmacokinetic properties of the drug, but new chemical molecules are formed after conjugation. The albumin drug delivery system in the form of physical combination is a more ideal drug loading mode. Encapsulating drug molecules in albumin nanoparticles can significantly improve the stability of water-insoluble drugs in aqueous solution (that is, in the blo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K31/337A61K31/12A61K9/16A61P35/00
Inventor 王文坦黄延宾程易
Owner TSINGHUA UNIV
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